We narrowed to 770 results for: VARS
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TypeBlog Post...students simply need to add water to express a variety of proteins. FD-CF reactions are also cost-effective..., but it could be widely adapted and used in a variety of settings. If you are a biology teacher at the...
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"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...go into developing gene therapy strategies for various diseases. That’s when I thought, if it cannot be...There should be ways to adapt the game to each a variety of subjects at many different education levels.... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...relax. Companies at LabCentral get access to a variety of experimental services and expertise without ... cancer. My partner for the day was Julie from Novartis who was working with cell culture and who was ... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...enhance expression of your gene of interest with a variety of Cas9 activators available in lentiviral format...be used to screen for genes involved in a wide variety of cellular processes. While these are widely requested... -
Hot Plasmids - January 2023
TypeBlog Post...Facility Isotype IgG2a Differences: Distinct variable domains that recognize different epitopes on GFP...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for... -
DAP arrays for multiplex gene editing
TypeBlog Post...mechanisms to enable multiplex gene editing in a variety of cells and organisms. However, lengthy genetic...gRNAs from DAP array.7 Using DAP arrays with a variety of base editors, the Gao Lab successfully demonstrated... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were... Various Addgenies... -
Management for Scientists: Delegating is Key
TypeBlog Post...efficiently. Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn...practices try "Why Aren't You Delegating" in the Harvard Business Review. Another excellent blog on delegation... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...bacterial cells); however, the techniques and reagents vary. Plasmid transfection into mammalian cells is fairly...performing a "kill curve" (basically growing cells in various concentrations of the selection reagent). Cells... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post...Excitation (dotted) and emission (solid) spectra for various teal, green, yellow, orange, and red fluorophores...Figure 4: Calcium titration curves for OCaMP and various red and yellow GECIs measured with soluble protein... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...exhibition area, I got a sampling of the broad variety of tools in neuroscience trade. There were, of ...easily I could jump in and ask questions about the various posters I visited. If you’re interested in neuroscience... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...plasmids can direct the production of virus with various tropisms. Packaging using helper-free packaging... virus is produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables ... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your samples... research technician in Arlene Sharpe's lab at Harvard Medical School. More resources on the Addgene... -
A Deep Dive into BioBuilder
TypeBlog Post...classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused on current ...100 teachers each year, with workshops held at various locations around the U.S. (and Mexico in 2018).... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...University of North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the... 2016 we surveyed hundreds of scientists about various ways we might expand Addgene’s services. There ... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it...CRISPR–Cas systems: a burst of class 2 and derived variants. Nature Reviews Microbiology, 18(2), 67–83. https... -
Viral Vectors 101: Viral Vector Elements
TypeBlog Post..., we are here to help with resources from AAV variables that matter to adenovirus production tips. For...DOI:10.3390/v13030388 Coffin J.M., Hughes S.H., Varmus H.E. Retroviruses. Cold Spring Harbor (NY): Cold... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...protein modifications of interest or any of a huge variety of ncAAs to study the relationship between structure... our blog – we’ll be posting more posts on the various protocols and helping to spread the word as new... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...University of California, Berkeley engineered an AAV variant that efficiently infects neurons, directs high-...Tervo, D. Gowanlock R., et al. "A designer AAV variant permits efficient retrograde access to projection... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post... confirm expression in target cells. While the various AAV serotypes can be used to achieve different ...supply of transgenic reporter lines that supply a variety of GFP expression patterns, thus allowing you to...