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We narrowed to 841 results for: ang

Showing: 441 - 460 of 841 results

  1. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...temperature parameters. For example, we found that changing the annealing temperature of the PCR from 60° ...primers can have different annealing efficiencies (Wang et al., 2013). Although you can optimize your primers...: 28546538. PubMed Central PMCID: PMC5445070. 6. Wang, Feng, et al. "A reliable and feasible qPCR strategy...

  2. How to Write a Scientific Review Article

    Type
    Blog Post
    ...popular papers, but looked for papers from a wide range of authors. Just start writing When I first started... and forth with. That being said, we probably exchanged dozens of drafts of the manuscript. This is the...transform the document into something cohesive-- change the sentences, make it flow, and start telling ...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes conjugated with its HaloTag...Through in vivo screening of human promoters, the Zhang Lab found a short fragment of human IBA1 promoter...functionally reconstituting coding sequences for a range of applications, including CRISPR activation by ...

  5. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...depositors David Liu, PhD, Keith Joung, PhD, and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for precise...Rinaldi F, Wein N, Meyer KC, Solano S, Nguyen K , Lang E, Kaspar AA, Foust KD, Thomsen G, Fugere M, Kaspar... Angela M. Holmes...

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...gene engineering to improve gene expression by changing synonymous codons based on an organism's codon...protein production in certain systems, synonymous changes to a gene sequence can cause unexpected detrimental...affect protein conformation, folding and stability, change post-translational modification sites and even ...

  7. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Jhunjhunwala, S., Aresta-Dasilva, S., Tang, K., Alvarez, D., Webber, M., Tang, B., Lavin, D., Veiseh, O., Doloff...Vegas, A., Ma, M., Sahay, G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    ...structures and compartments in mammalian cells By Angela Abitua Genetically encoded fluorescent proteins...system for human pluripotent stem cells By Jennifer Tsang CRISPR gene interference (CRISPRi) and gene activation...s41598-020-57500-1 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids...

  9. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... Many people use 'labeling' and 'staining' interchangeably to refer to any technique that combines a marker...conjugates are commercially available for a wide range of fluorophores, allowing for great flexibility ...epitope-targeting antibody can originate from a range of species; for example, rabbit or mouse. In a second...

  10. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...exceptional brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM,...PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly...Improving the photostability of bright monomeric orange and red fluorescent proteins." Nature methods 5.6...

  11. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...nature. Once the bond is formed it is unaffected by changes in pH, temperature or organic solvents. Uses of... 25980573. PubMed Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu, Y. “A non-radioactive...PubMed Central PMCID: PMC164612. 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated...

  12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...under blue light BcLOV4 undergoes a conformational change and binds the plasma membrane under blue light ...family signaling pathways. Rho guanine-nucleotide exchange factors (GEFs) activate their partner Rho GTPase...formation, cell contraction, or other morphological changes, depending on the specific Rho GTPase/GEF pair ...

  13. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...of peer reviews so we can see how and why papers change throughout the review process. This will hopefully...problem in Button et al 2013 and Ioannidis 2005) and changing an experimental set-up partway through an experiment... the experiments they’re reading about weren’t changed halfway through in order to chase a positive result...

  14. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...First, you’d construct a plasmid with your desired changes, long homology arms and a selection marker, then...creating a conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic...assistance with editing this blog post. References Yang, Hui, et al. "One-step generation of mice carrying...

  15. A Control for All Seasons

    Type
    Blog Post
    ...purple) and nonspecifically through the Fc region (orange). The Preimmune serum control will not bind to ...the label control for all new samples, protocol changes, or when high background labeling occurs (Burry...critical for data interpretation, especially when changes in protein expression are the experimental readout...

  16. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...giving greater concentration of cut DNA without changing the size. As a result, a restriction digest of... Resources and References References Bacolla A, Wang G, Jain A, Chuzhanova NA, Cer RZ, Collins JR, Cooper...19233255; PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid...

  17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...temperatures (up to 95 °C) and is active over a range of elevated temperatures (37–75 °C). T4 DNA ligase... PubMed Central PMCID: PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection...26365241. PubMed Central PMCID: PMC4737175. 22. Liu, Pingfang, Artur Burdzy, and Lawrence C. Sowers. "DNA ligases...

  18. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...exciting news for you too! The CRISPR field is always changing so we’ve been busy updating our CRISPR guide. ... Jennifer Tsang...

  19. The Twelve Days of CRISPR

    Type
    Blog Post
    ...new CRISPR tools. Since the CRISPR field keeps changing, we’ve been updating many of these posts over ... Jennifer Tsang...

  20. Addgene at SfN23!

    Type
    Blog Post
    ...Content, Brook, and our scientific curation manager, Angela, experts on the Addgene repository, are also attending...development workshop. Specifically, Jo, Brook, and Angela are co-leading "Making a Greater Impact Using Clear...
Showing: 441 - 460 of 841 results