...exceptional brightness, photostability and dynamic range: characterization of dedicated sensors for FLIM,...PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly...Improving the photostability of bright monomeric orange and red fluorescent proteins." Nature methods 5.6...

...nature. Once the bond is formed it is unaffected by changes in pH, temperature or organic solvents. Uses of... 25980573. PubMed Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu, Y. “A non-radioactive...PubMed Central PMCID: PMC164612. 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated...

... Many people use 'labeling' and 'staining' interchangeably to refer to any technique that combines a marker...conjugates are commercially available for a wide range of fluorophores, allowing for great flexibility ...epitope-targeting antibody can originate from a range of species; for example, rabbit or mouse. In a second...

...under blue light BcLOV4 undergoes a conformational change and binds the plasma membrane under blue light ...family signaling pathways. Rho guanine-nucleotide exchange factors (GEFs) activate their partner Rho GTPase...formation, cell contraction, or other morphological changes, depending on the specific Rho GTPase/GEF pair ...

...of peer reviews so we can see how and why papers change throughout the review process. This will hopefully...problem in Button et al 2013 and Ioannidis 2005) and changing an experimental set-up partway through an experiment... the experiments they’re reading about weren’t changed halfway through in order to chase a positive result...

...First, you’d construct a plasmid with your desired changes, long homology arms and a selection marker, then...creating a conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic...assistance with editing this blog post. References Yang, Hui, et al. "One-step generation of mice carrying...

...purple) and nonspecifically through the Fc region (orange). The Preimmune serum control will not bind to ...the label control for all new samples, protocol changes, or when high background labeling occurs (Burry...critical for data interpretation, especially when changes in protein expression are the experimental readout...

...giving greater concentration of cut DNA without changing the size. As a result, a restriction digest of... Resources and References References Bacolla A, Wang G, Jain A, Chuzhanova NA, Cer RZ, Collins JR, Cooper...19233255; PMCID: PMC2693335. Williams JA, Luke J, Langtry S, Anderson S, Hodgson CP, Carnes AE. Generic plasmid...

...temperatures (up to 95 °C) and is active over a range of elevated temperatures (37–75 °C). T4 DNA ligase... PubMed Central PMCID: PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection...26365241. PubMed Central PMCID: PMC4737175. 22. Liu, Pingfang, Artur Burdzy, and Lawrence C. Sowers. "DNA ligases...

...exciting news for you too! The CRISPR field is always changing so we’ve been busy updating our CRISPR guide. ... Jennifer Tsang...

...new CRISPR tools. Since the CRISPR field keeps changing, we’ve been updating many of these posts over ... Jennifer Tsang...

...Content, Brook, and our scientific curation manager, Angela, experts on the Addgene repository, are also attending...development workshop. Specifically, Jo, Brook, and Angela are co-leading "Making a Greater Impact Using Clear...

...producer for the Addgene Podcast.     References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system ...Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “Cell type-specific manipulation...

...generally listed as the amino acid change and not a nucleotide change. The proper way to denote an amino...

... Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform called SHERLOCK... Jennifer Tsang...

...concerned about these developments. These abrupt changes threaten to slow transformative biological discoveries... invite researchers to share how these funding changes are impacting your work and what support would ...

... capital firm, Flagship Ventures, Koeris helped change Novophage's focus. The company went from developing...pathogens in food products. Along the way, the company changed its name from Novophage to Sample 6. To learn ...

...Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad Institute of MIT and Harvard and the...depositing scientist – stop by our booth to say hi! Zhang was awarded one of this year’s SfN Young Investigator...

...and electrocompetent cells is minimal (picogram range). It can be extracted from small bacterial cultures...scientists to clone from a smaller amount of DNA (ng range). To allow efficient and flawless cloning it is ...

...available for genome-wide studies in E. coli, the Chong Zhang lab developed a genome-scale CRISPR inhibition pooled... Jennifer Tsang...