We narrowed to 536 results for: val

1. Open Resources and Plasmid Tools For Studying C. elegans

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   ...domains that naturally “associate, self-excise, and covalently ligate their flanking peptides together (Wang...

2. Hot Plasmids - October 2020

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   ...in length to tag the target protein. The team validated this tool by targeting six different genes whose...

3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...-x Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD (2014) A Protein-Tagging System for Signal Amplification...

4. 6 Tips for Grant Writing

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   ...choose where you go carefully. Funding agencies want value for their money so you need to make it clear that...

5. Fluorescent Proteins 101: Introduction to FRET

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   ...well-characterized FRET reference standards can be used to validate FRET measurements and serve as a type of positive...

6. Adapting Toehold Switches to Detect Zika Virus

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   ...information from public databases to the production of validated sensors in as little as one week (Pardee et al...

7. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ...Pages Check Out MpAgo from the Doudna Lab Find Validated gRNAs ...

8. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   ...barely heard of these). These single stranded, covalently closed RNAs seem to be conserved in mammals, ...

9. The Materials Science of Optogenetics Experiments

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   ...surgeries and standard molecular neuroscience validation experiments we discussed last week are only half...

10. Experimenting in an Open Source lab: from CRISPR to Cats

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    ..., and a metalshop. After two summers in Silicon Valley as a student and then instructor at Singularity...

11. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    ...flower that changes color, but the experience is invaluable. This opportunity has given us the chance to ...

12. Hot Plasmids: Winter 2025

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    ...of Tau (phospho-Tau). We hope these will become valuable tools for your research! If they do (or if they...

13. Common Injection Routes in Mice

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    ...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good...

14. Pooled Library Amplifications

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    ...amplification protocols attached.  Final step: validation  Remember, there is no “one-size-fits-all” protocol...

15. Twenty Years of Addgene Sharing: CRISPR

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    ...Directed Repair has been viewed 219,289 times, while Validating Your Genome Edit has 76,011 views.  Has all of...

16. Plasmids 101: Degron Tags

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    ...conditional degron tag technologies for target validation studies. Nat Commun 13, 5495 (2022). doi:10.1038...

17. Viral Vectors 101: Voltage Indicators

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    ...specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic...

18. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...experimental data of its effect on HDR efficiency.  These values (ranging from -40 bp to 40 bp) are also converted...

19. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    ...re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment test systems was...

20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ... of phage, increasing stringency and promoting survival of only the broadest-targeting variants. For the...