Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,010 results for: RING;

Showing: 231 - 240 of 1010 results

  1. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... Figure 1: Addgene’s Agile Approach Sharing is caring There are many moving parts to our software...overall soundness of the software design. Each of us brings slightly different cognitive biases as a result...defined chunks, called stories, and work on stories during short periods of time called sprints. The team ...different technical approaches that may come up during discussion.  One example of agility at work is ...we recently reduced the number of steps involved during checkout. Demo details for this would be something...diversifying our own knowledge of the software, sharing knowledge allows for life to happen without impacting...developing new features and fixing bugs: we take time during each sprint to do some maintenance work on our ...

  2. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...Wisconsin lab for a summer during my PhD and is responsible for my enduring love of cheese curds. We’...long line for bag check was definitely worth it! Hearing about his passion for global health and the various...one of the highlights of the conference. It was inspiring to see how microbiology research is improving ...researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors who stopped...leverage their expertise as we advance our mission of sharing science. I got to check out many of the posters...about our mission of open science and resource sharing and was also a great place to catch up with former...distribution service! Did you see a must-have plasmid during ASM? Let us know what’s missing in the collection...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...combines CRISPR and recombineering-based MAGE (Multiplex Automated Genome Engineering) technology. pMA7CR... a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" after induction with...CRMAGE is much more efficient than traditional recombineering, with 96-99% efficiency for point mutations...for a single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation...References 1. Maddalo, Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with ..., et al. “Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids.... Sakuma, Tetsushi, et al. “Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector...

  4. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made targeted...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ... fill in the resulting ssDNA. A single HJ forms during this process and resolves by cleavage of the crossed...immediately applicable in DSB-induced genome engineering, it has biological importance for the repair ...intact. In other words, Cas9 will keep cutting and repairing your target DNA until a mutation is introduced...Homologous Recombination. Jasin M and Rothstein R. Cold Spring Harb Perspect Biol (2013). PubMed. DNA double-strand... (2018). Pubmed. CRISPR References: Genome engineering using the CRISPR-Cas9 system. Ran FA, et al. ...

  5. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...other to be physically present at certain times during the day? We asked three lab heads how they go about... If you are looking to join a lab, you may be wondering “What is this PI looking for in a new researcher...of CVs collected from throughout the year since hiring opportunities arise at various times. If the timing...will provide a solid foundation on which to thrive during time spent as a graduate student or postdoc. Although...chosen research field is important, the end goal during this time is to be trained to think and execute.... Or perhaps, the lab has a reputation for not entering collaborations. Alternatively, perhaps the lab...to ask lab heads, lab members and lab neighbors during the interview process. What if I want a non-academic...

  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...methods for physical shearing exist as well, such as sonication and needle shearing. Essentially, any method... with the lab during its recent move to the University of Georgia). She enjoys pondering how phages get...between 1 and 3 kb results in optimal likelihood of capturing a whole RBP gene without causing issues associated...following colony PCR of 10-20 colonies using primers bordering the multiple cloning site of the vector. In order...saturate each with lysis solution (~3 ml each), pouring off any excess. When your protein induction is .... Leaving the membrane overnight in the freezer during one of these steps is also a convenient stopping...through the day, spending time with her horse, and wondering what her cat is thinking. Find her on Twitter ...

  7. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...some of these questions in the sections below. Mentoring relationships can help identify your priorities...Alumni meetings can be the most informative way of gathering data. Former lab members can give open, revealing... Know who's boss You sign on to a lab for the mentoring only to find out that the lab head is an absentee...You are not allowed to speak to lab members alone during the interview period. People seem to be hiding ...about discussing data. There’s hesitation before answering questions about lab culture. Support for diverse...ensure that you are expanding your options and preparing for both academic and non-academic career paths...evidence of industry collaboration? If you are considering a career at a small, teaching college you might...

  8. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression of...interfere with the precision needed in metabolic engineering or other synthetic biology circuits. What is ... a look at how the stabilized promoters perform during these types of fluctuations. When cells are dividing... can also cause changes in plasmid copy number. During the shift from exponential to stationary phase,...promoters were able to better modulate gene expression during shifts in growth rate as compared to a constitutive...To simulate the increase in plasmid copy number during the transition to stationary phase, Segall-Shapiro...they could alter copy number of the plasmid by altering IPTG concentrations. Higher IPTG concentration...

  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...leads to incomplete reprogramming. Additionally, lingering expression or re-expression of viral transgenes...temperature sensitive Sendai virus which is removed by culturing cells at 38℃, but screening cells for the presence...considered the safest since there’s minimal risk of lingering expression of reprogramming factors. Non-replicating...the plasmid in mammalian cells and assists in tethering the vector to the cell’s chromosomes. With the... high marks for safety.   Protein Delivery: Delivering reprogramming factors as protein is another way... week for 6 weeks, and still required further culturing to generate iPSC colonies. Reprogramming efficiencies...three germ layers (ectoderm, mesoderm, endoderm). Measuring pluripotency of mouse cells typically involves...

  10. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...makes sense that scientists, many of whom are venturing into genome editing for the first time, have lots... the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems has expanded out of ...wildtype or double nickase for my CRISPR genome engineering experiments? A1: When assessing which nickase...nickase type to use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized...amazing resources for the scientific community and sharing these resources with Addgene. Thank you, Le! This...K., & Carroll, D. (2013). Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes... L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/CAS systems. Science, 339(6121),...
Showing: 231 - 240 of 1010 results