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We narrowed to 928 results for: INA

Showing: 691 - 720 of 928 results

  1. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...the actions of a single person but through the coordinated efforts of many individuals. Check out Joanne's... a project progresses it is easy to forgot the original plan. Write down your plans in a shared document...

  3. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...dCas9 fused to the Activation Induced Cytidine Deaminase (AID) enzyme. AID, however, only changes cytidines...targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard mutation ...

  5. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...writing grant proposals to be reviewed by interdisciplinary panels.  And highly successful scientists ... graduate students in different regional and disciplinary communities gather at ComSciCon's local and ...

  6. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... very similar to the widely used FRET, but it eliminates the need for an excitation light source. Instead... medium to allow RLuc8 to produce photons. The original nano-lantern emitted yellow-green light and exhibited...

  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...from Bryan Roth’s lab at the University of North Carolina, Chapel Hill. These can be used to investigate... only be expressed in cells that express cre recombinase. Typically, this feature is used to attain cell-specific...

  8. Hot Plasmids - February 2022

    Type
    Blog Post
    ...identifying guide selection rules, and reducing recombination by finding acceptable variations in DR sequences... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...

  9. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...dissociation from the GPCR allows for binding of a new inactive heterotrimeric G protein complex and subsequently...pathways from ligand to receptor to G-protein, and finally, to a response? Let’s take a look at a few examples...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...applications Vaccine development Attenuated and inactivated viruses pioneered vaccine development well over...

  11. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward across...for multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons ...

  13. Clear and Accessible Writing

    Type
    Blog Post
    ...website, as an instructive step-by-step guide.  Finally, when writing your alt text, use all of this information... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ...

  15. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Ku then acts as a scaffold for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...

  17. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...plasmids out of the library in the appropriate combination and do a restriction enzyme digest to cut out...scientists relied on traditional homologous recombination. That technology had been used for decades to...

  18. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...LinkedIn may revoke your ability to send invitations. Finally, if you don’t know clearly why you are inviting...updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment...

  19. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ... DNA that you want to assemble together in a combination of different ways according to your cloning strategy... or PCR products from a gel. You will avoid contamination from other DNA fragments and you will remove...

  22. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...expressed. Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy is induced. Then non-selective... Pho8 from its uncleaved to cleaved form is the final read out of this assay. This is also a quantitative...

  24. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...when designing your primers can be found here. Finally, you will want to have a well-organized database...

  28. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...carrying through on the actions you committed to. Finally, hiring a paid coach will make you take the experience...sensitive to this and have coaching plans that are financially accessible. Sometimes only a few hours of coaching...

  30. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...enable us to measure these properties, and what fascinating experiments could push these tools even further... Förster Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation...
Showing: 691 - 720 of 928 results