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We narrowed to 922 results for: Por

Showing: 691 - 720 of 922 results

  1. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...all of which need to be represented in the same proportion after amplification as they were prior to amplification...this guide, we hope you feel more confident incorporating them into your research. And, of course, if ...

  2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... example all C’s will edit to T’s. Thus, it’s important that there are no “bystander bases” if you choose... particular gene isn’t feasible. For example, temporary repression or knockdown allow you to study essential...

  3. How to Write a Scientific Review Article

    Type
    Blog Post
    ... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently...

  4. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...coding sequence. If you choose this strategy, it’s important to include the proper protein expression elements...Clone When making the expression clone, it is important to choose the destination vector that best fits...

  5. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...and not the whiteboard. Thank everyone for the opportunity to share your research vision with them. Do not...proud of it and be excited about getting the opportunity to realize your vision. Many thanks to our...

  6. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ... More recently, the Bird lab found that only a portion of the MECP2 protein is necessary to rescue the...Neurological Disorder by Programmable RNA Editing. Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep.2020.107878...

  7. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...sharing their ideas? And how do we give people the opportunity to speak out and prevent groupthink? Here are...implemented, impacted the entire company. It is important to note that the person who submitted the idea...

  8. The Challenges of Cell Culture

    Type
    Blog Post
    ...been known about since 1966 when Stanley Gartler reported that most of the 18 cell lines he had studied ...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...single-use aliquots, and stored at -80°C. Some reports suggest rapidly freezing virus in a dry ice/ethanol...protein attaches to cell surface receptors, other important binding events take place during the course of...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...added to the growth medium. Induction timing is important, as you typically want to make sure your cells... optimal because of the high cell-density they support; however, minimal media supplemented with M9 salts...

  12. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... theory, is an easy technique, but it is very important to follow some simple rules to avoid common pitfalls...purpose of your studies. These parameters are less important when designing a biosensor, as the distance between...

  13. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... to therapeutic uses, and in those cases it's important to identify the right tissue-specific promoters...Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org...

  14. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... out of fashion but is still useful and most importantly, effective. In the research world, there is a...plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence Choosing ...

  15. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...purification and minimizes sample loss while also supporting imaging, making it easy to identify dozens or...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...

  16. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...option for the experiment above, but it’s also important not to load yourself up with too many tasks. If...ll discuss in the planning section below, it’s important to dedicate specific amounts of time to specific...

  17. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an antigen of interest...interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction and release...

  18. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... areas of microbial research like metabolism. Transport, biofilms, and much more. Because of it’s BSL-...designed for use in Arabidopsis. There are also important tips for plant transformations and the use of ...

  20. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...merely co-opted toward that aim (i.e. sacB). Importantly, to prevent activation of toxins before the second...genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason...

  21. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend...be cut with both enzymes, and therefore it is important that the digest go at least 4 hours and as long...running a gel for purification purposes it is important to have nice crisp bands and to have space to ... your favorite gel purification method, it is important to determine the concentration of recovered DNA...ratios of recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance...

  22. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...better to reduce off-target effects, but it’s important to not let ‘perfection’ get in the way of ‘good... score their probability of off-target events: CRISPOR, Cas-OFFinder, CCTop, and many more. While such...

  23. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...protocol is a good place to get started.  One important consideration when deciding whether you can do...specific considerations to keep in mind. Most importantly, make sure you are using primary antibodies from...

  25. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...around the band as possible. To do so, it is often important to take the excised band, lay it down on the UV...sides with the razor blade. This is especially important during the DNA purification step, as many kits... fragment. The weight of the gel is directly proportional to its liquid volume and this is used to determine...manufacturer's instructions. Note: It is usually important to determine the concentration of the DNA that...

  26. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...a pressurized buffer called sheath fluid and transported through tubes or capillaries to a laser. As the...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...

  27. Protocol - Bacterial Transformation

    Type
    Protocol
    .... Transformation of bacteria with plasmids is important not only for studies in bacteria but also because...amplification. Higher efficiency cells are more important if you will be transforming with very small amounts...critical for Ampicillin resistance but is much more important for other antibiotic resistances. Plate some or... do this you will need to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's...

  28. Using a Light Microscope Protocol

    Type
    Protocol
    ...light to detect small objects. Two of the most important properties of a microscope are magnification (...see several pieces of information, but the most important (for now) is the magnification power, such as ...be sure to carry it with two hands - one hand supporting the base and the other holding the arm - or use...your images in the future. Conclusion Like any important laboratory instrument, you should be sure to take...

  29. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...refer to Cong et al. (2013) for more details. One important note is that this ‘split RNA’ design is not as.... (2013). One-Step generation of mice carrying reporter and conditional alleles by CRISPR/CAS-Mediated...

  30. Lentivirus ddPCR Titration

    Type
    Protocol
    ...EX0276-1 Benzonase 250 U/µl, Millipore #71205-3 Polybrene 10 mg/mL, Millipore, TR-1003-G Molecular Biology...place the PCR plate with the foil onto the metal support block. Place the block in the plate sealer and ... reading. When the droplet reading is complete, export the data from all wells as a CSV file which will...
Showing: 691 - 720 of 922 results