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We narrowed to 954 results for: app

Showing: 691 - 720 of 954 results

  1. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...

  3. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer...

  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Wickersham et al., 2007).   Summary and RABV applications Use of G deleted RABV in conjunction with restricted...L, Ogawa SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. ...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    ... FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein...

  10. Tips for Technical Support Calls

    Type
    Blog Post
    ..., I’m confident that these tips and tricks will apply to any biology-related customer support interaction...company. This might seem like a no-brainer, but it happens more frequently than you might think. The Addgene...

  12. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here THE ORIGINAL ...the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing HDR efficiency...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...characterization of the location, morphology, and circuit mapping of a targeted neuronal population.    Figure... cell types for imaging and long-range circuit mapping. PubMed PMID: 18614669. PubMed Central PMCID: PMC2593125...

  15. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...

  16. Pooled Library Amplifications

    Type
    Blog Post
    ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...feel free to contact us at [email protected]. Happy amplifying! More resources on the Addgene blog ...

  17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...possible consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, Subach ...-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein timers FP timers have...

  18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ... disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers two...single-gene knockdown experiments. Mobile-CRISPRi applications Mobile-CRISPRi expands CRISPRi to non-model ...

  20. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... just trying to create a knockout, you might be happy targeting anywhere in an exon. But things get more...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

  22. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...waste should be autoclaved before being disposed Happy culturing! Many thanks to our guest blogger, Sana...expression disregulation on HeLa cells." Journal of applied phycology27.6 (2015): 2321-2332. 3. Contamination...

  23. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...shadows along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to...

  25. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

  26. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...101: Types of Viruses Viral Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral...Liu, H., Xiao, H., Conway, J. A., Hunter, E., & Kappes, J. C. (1997). Functional RT and IN incorporated...

  27. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...and retroviral expression system for using this approach in in vivo studies (Fujita et al., 2018). In addition...DUX4, a transcriptional regulator that when inappropriately expressed can cause facioscapulohumeral muscular...

  30. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...expression observed using transient transfection approaches, generating cell lines using lentiviral vectors... tubes, VWR 21008-216 Lentivirus preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you.... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. This is the beginning of the selection...
Showing: 691 - 720 of 954 results