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We narrowed to 960 results for: App

Showing: 461 - 480 of 960 results

  1. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...photoconverted, so this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh...

  2. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...to explore the paired double-strand break (DSB) approach at a variety of loci. We were pleasantly surprised... size and frequency [3]. There are several applications in which the production of deletions by paired... RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites within a single ...

  3. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...were willing and able to listen to Addgene’s new approach of working with the industry-based scientific ...reduced to days and weeks. As of June 2020, we are happy to have the support of the following organizations...project is complete, Ginkgo will have synthesized approximately 3,000 constructs to support SARS-CoV-2 research...

  4. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ... value and priority put on good mentoring and applauded the new requirement for PIs to include in their... of funding basic research at NEB and got some applause when he talked about how he boycotts publishing...opportunities.  Scientists must do science to be happy so the beginnings of solutions can’t come too soon...

  5. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the...expression level. Labs have been trying different approaches to improve CTC capture, but I hope that our antibody...

  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...called protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing... are recognized by the Cas9 protein from the appropriate species. Some Cas proteins, like LbCas12a or ...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

  9. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...aggregation, it’s recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al...flames,” it’s clear that the system is broadly applicable across many areas of research. Beyond fluorescence...activation of target genes, but we’ll save that application for another day! References 1. Tanenbaum, Marvin...

  10. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...been requested 100+ times Since we so sincerely appreciate our depositor’s dedication to scientific sharing...plasmids with us. One way we can gauge if people are happy depositors is if they deposit plasmids more than...off! To share your photo, tag @Addgene and #happydepositor on Twitter. All Blue Flames started with a ...

  11. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...inflammation, and neurotransmission. But with approximately 950 different human genes encoding GPCRs (Takeda...GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2 and Gβ constructs. Cells are then treated...plasmids in a number of different experimental approaches. They first assessed the activation of different...

  12. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...as #happydepositors who stopped by to say hello. Needless to say we all got very good at applying temporary...six continents who dropped by the booth. We were happy to chat with lots of researchers who weren’t aware...

  13. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ... though not all, of these defenses. For some applications of genome engineering - such as certain forms...and improved SB transposase, SB100X, that is approximately 100-fold more efficient than the first-generation...Epub 2009 May 3. PubMed. 3. Singh H, et al. A new approach to gene therapy using Sleeping Beauty to genetically...

  14. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...team. It is pretty clear that if people are mostly happy at work, they will work harder and contribute more...contribute to job satisfaction: A feeling of being appreciated and valued This often stems from being included... Need feedback on their work but prefer to be approached as peers rather than subordinates Need mental...

  15. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... supplies every 10 years or so. Actually, I was happy to hear that I am not the only Addgenie who has ...I started looking for smaller and smaller gel apparatus until I settled on using glass slides. With just...this one only cost a few dollars.  I used this apparatus for 15 years before I hung up my pipets.   ...

  16. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...form has 5 general question types and it takes approximately 15 minutes or less if you have all of your data...under copyright, you will need to obtain written approval from the copyright owner and will have the opportunity...viewing the data you have any feedback at all, we’d appreciate you sharing it through this form. In honor of...

  17. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...different levels of apparent dopamine affinity, with 113050 offering high apparent affinity, and 113049...113049 offering medium apparent affinity. Plasmid Serotype Name 113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h...

  18. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...

  19. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...of an experimental protein-ligand interaction approaches that of GST-glutathione. Biotin-based immobilization...lack of site selectivity typically diminishes the apparent affinity for the ligand, especially if the modified...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which...

  20. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...artefacts in your experiments. Read this post from Erik Snapp, Associate Professor at Albert Einstein College ... membranes. Read the full post for potential applications of R-bodies and to find a video of them in action... to your DNA sequence of interest. Potential applications of the system include activation and repression...
Showing: 461 - 480 of 960 results