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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 772 results for: Dos

Showing: 461 - 480 of 772 results

  1. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...being published on our own topic. Only on occasion do we then have time to read about research from other...otherwise never learn from the valuable research we are doing. Browse the site, learn from others, share ideas...

  2. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...editor. Cas13 enzymes are advantageous because they do not require a PAM, and RNA targeting is potentially...flanking sequence (PFS) adjacent to the target, but many do not, showing the flexibility of this system.  As ...

  4. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...tool that is easy to use with other plasmids.  How do you know if your plasmids are incompatible? Scientists...will be based on sequence data. However, if these do not provide clarity, a wet lab transformation experiment...

  5. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... using site-directed mutagenesis of Spinach. In doing so, they identified the nucleotides that contribute...Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in 2017 (Song...

  6. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...sites, or you can utilise several online tools that do it for you. One such option is the CRISPR Design ... take roughly 3 months from the day you decide to do a gene modification experiment in mice to the day...

  8. Addgene's Website Update

    Type
    Blog Post
    ...Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as well as information...

  12. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...alone. (Seriously, is there anything CRISPRs can't do?) Structural details All this, and scientists are...

  13. CRISPR Plasmids - Tagging

    Type
    Collection
    ... homology arms and EGFP are available at Addgene. Do you have suggestions for other plasmids that should...

  17. Let There Be LITE Plasmids

    Type
    Blog Post
    ...tools in C. elegans and in Plasmodium. For those who do want to apply LITEs for the transformation of neurons...exposure. Third, the response can be graded with light dose and is fully reversible after light retraction. ...

  18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular brightness...

  19. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...selection: labeling markers that your cells of interest do not have, and selecting for the cells that are not...cytosolic proteins alongside the intranuclear ones. You do not need to use a separate fix/perm cytosolic procedure...
Showing: 461 - 480 of 772 results