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Open science resources like Addgene's Data Hub increase reproducibility, reduce validation and optimization timeframes, and drive science forward.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Use these CRISPR base editing reporters to track base editing events in real-time and enrich for base edited cells.

Plasmid incompatibility is the inability of different plasmids to be maintained in a cell. We’ll cover why this happens and how it might affect your experiment.

Aptamers are nucleic acid oligos that bind to small molecules to induce their fluorescence and can be used to study RNA localization and dynamics.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

Learn why it's a good idea to share your science through video and check out the new research video sharing platform We Share Science.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

This 3rd and final post of our Primer on Optogenetics has tips to help you design your own optogenetics behavioral experiment.

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

addgene.org just got a new look!

This blog series showcases some of the new CRISPR tools we have at Addgene each quarter.

Addgene participated in the 2015 MassBioEd career exploration day to teach students some of the great careers available in biology.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Inntags are new epitope tags designed to minimally interfere with protein folding. Find out more about Inntags in this blog post.

Science in the News (SITN), a Harvard group dedicated to making science understandable, recently hosted DayCon, a science conference for the general public.

Find tips for using the chromatogram to analyze your Sanger DNA sequencing results.

CRISPR-Cas9 technology appears in numerous publications, including its use for genome editing in monkeys and x-ray crystallography of Cas9's 3-D shape.

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

Learn about flow cytometry labeling strategies beyond extracellular markers in this Antibodies 101 post!