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We narrowed to 770 results for: Dos;

Showing: 461 - 480 of 770 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...editor. Cas13 enzymes are advantageous because they do not require a PAM, and RNA targeting is potentially...flanking sequence (PFS) adjacent to the target, but many do not, showing the flexibility of this system.  As ...

  3. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...being published on our own topic. Only on occasion do we then have time to read about research from other...otherwise never learn from the valuable research we are doing. Browse the site, learn from others, share ideas...

  4. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...sites, or you can utilise several online tools that do it for you. One such option is the CRISPR Design ... take roughly 3 months from the day you decide to do a gene modification experiment in mice to the day...

  6. Addgene's Website Update

    Type
    Blog Post
    ...Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as well as information...

  10. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...alone. (Seriously, is there anything CRISPRs can't do?) Structural details All this, and scientists are...

  11. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...single-stranded DNA (ssDNA) genome. Non-enveloped viruses do not have a lipid bilayer surrounding the viral particle.... AAVs are therefore non-pathogenic and generally do not trigger a strong immune response. These features... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...vectors present low oncogenic potential because they do not integrate in the human chromosome, which reduces...

  15. Let There Be LITE Plasmids

    Type
    Blog Post
    ...tools in C. elegans and in Plasmodium. For those who do want to apply LITEs for the transformation of neurons...exposure. Third, the response can be graded with light dose and is fully reversible after light retraction. ...

  16. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular brightness...

  17. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...small probes that are entirely genetically encoded do not exist, but the development of non-natural fluorescent...functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal fusions (Adjobo-Hermans...

  19. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...selection: labeling markers that your cells of interest do not have, and selecting for the cells that are not...cytosolic proteins alongside the intranuclear ones. You do not need to use a separate fix/perm cytosolic procedure...

  20. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...RGD-binding integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the...function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived antibodies fall into...
Showing: 461 - 480 of 770 results