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  2. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...cloning and expression from plasmids, due to only needing one open reading frame Ability to access unique...to producing single-domain antibodies without the need for a species that naturally produces heavy-chain...

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...starts with a 'G', you naturally have one and do not need to add an additional 'G'. Q3: What is the maximum...More FAQs, CRISPR protocols, and gRNA design tools Need more questions answered about CRISPRs? Check out...

  4. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...advisors they choose will be ones that help them succeed after the training period. While there are many...is important in both cases. As a PhD student you need to learn how to be a scientist. Your PI should be...

  5. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...many microns in response to pH changes without the need for ATP or other chemical fuel sources.” In her ... how you can customize it for your experimental needs. Note: Casilio is no longer available through Addgene...

  6. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ..., making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging...adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid...

  7. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ...isotope conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t ...this will not affect single-plexed experiments, it needs to be addressed when multiplexing. It is imperative...

  9. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...should be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In...Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it seems that ...

  11. Advice for Moving Into Sales After Your Science Postdoc

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    Blog Post
    ...colleagues and what I was researching, but I decided I needed a new challenge. Looking for sales and marketing...opportunities to expand their skill sets?", “Have they succeeded in what they have done?” and “Would their academic...

  12. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post
    ...YOUR PLASMID BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, ...and one “Transfer Plasmid” game board. Once they agreed on each element to use, they had to place the selected...

  13. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post
    ...misfolding and recombination during virus production needed to deliver CRISPR-Sirius into cells. They named...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United...

  15. Summer SciComm Series: A PhD in Science Communication

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    Blog Post
    ...can tailor our communications to better meet the needs of the people we are talking to. Emma Harcourt ...polarized beliefs on controversial science topics. Proceedings of the National Academy of Sciences of the United...

  17. Hot Plasmids - January 2023

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    Blog Post
    ...another option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated...Stroik Microscopy techniques have been moving at the speed of light over the last decade, but the fluorescent...

  18. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... was surprised by the variety of skill sets she needed to acquire as a professor. “Being a PI is more ...“how much better it is once it gets fully up to speed.” He also comments that it is also surprising “ ...

  19. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...a lab that fits both work style and development needs. These two factors will provide a solid foundation...prefer a more competitive style to spur you to succeed or you might enjoy working closely with others ...

  20. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...similar to the widely used FRET, but it eliminates the need for an excitation light source. Instead, a luciferase...Saito, Kenta, et al. “Luminescent proteins for high-speed single-cell and whole-body imaging.” Nat Commun....
Showing: 461 - 480 of 816 results