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We narrowed to 829 results for: EED

Showing: 461 - 480 of 829 results

  3. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... often used interchangeably with the term sgRNA. Seed sequence: The 8-10 bases on the 3’ end of an RNA...CRISPR Cheat Sheet  Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

  4. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...cloning and expression from plasmids, due to only needing one open reading frame Ability to access unique...to producing single-domain antibodies without the need for a species that naturally produces heavy-chain...

  5. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. Here’s what you’ll find in this article: CRISPR-based...catalytic activity.  As SLs are also known to trigger seed germination of parasitic plants, this approach was...

  9. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...advisors they choose will be ones that help them succeed after the training period. While there are many...is important in both cases. As a PhD student you need to learn how to be a scientist. Your PI should be...

  11. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ..., making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging...adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer plasmid...

  12. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...isotope conversion, gives users the flexibility needed for multiplexing. In the case above, you can’t ...this will not affect single-plexed experiments, it needs to be addressed when multiplexing. It is imperative...

  13. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...many microns in response to pH changes without the need for ATP or other chemical fuel sources.” In her ... how you can customize it for your experimental needs. Note: Casilio is no longer available through Addgene...

  14. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...should be stable in the cell. gDNA guides will also need to be transfected into the cells of interest. In...Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it seems that ...

  16. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...colleagues and what I was researching, but I decided I needed a new challenge. Looking for sales and marketing...opportunities to expand their skill sets?", “Have they succeeded in what they have done?” and “Would their academic...

  17. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...misfolding and recombination during virus production needed to deliver CRISPR-Sirius into cells. They named...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United...

  19. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...can tailor our communications to better meet the needs of the people we are talking to. Emma Harcourt ...polarized beliefs on controversial science topics. Proceedings of the National Academy of Sciences of the United...

  20. Hot Plasmids - January 2023

    Type
    Blog Post
    ...another option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated...Stroik Microscopy techniques have been moving at the speed of light over the last decade, but the fluorescent...
Showing: 461 - 480 of 829 results