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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 825 results for: EED

Showing: 461 - 480 of 825 results

  2. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...colleagues and what I was researching, but I decided I needed a new challenge. Looking for sales and marketing...opportunities to expand their skill sets?", “Have they succeeded in what they have done?” and “Would their academic...

  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ...another option for targeting GFP. But why might you need a second GFP antibody?  Similarities:  Generated...Stroik Microscopy techniques have been moving at the speed of light over the last decade, but the fluorescent...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...misfolding and recombination during virus production needed to deliver CRISPR-Sirius into cells. They named...labeling of chromosomal loci in human cells.” Proceedings of the National Academy of Sciences of the United...

  7. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...can tailor our communications to better meet the needs of the people we are talking to. Emma Harcourt ...polarized beliefs on controversial science topics. Proceedings of the National Academy of Sciences of the United...

  8. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...YOUR PLASMID BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, ...and one “Transfer Plasmid” game board. Once they agreed on each element to use, they had to place the selected...

  9. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... was surprised by the variety of skill sets she needed to acquire as a professor. “Being a PI is more ...“how much better it is once it gets fully up to speed.” He also comments that it is also surprising “ ...

  10. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...a lab that fits both work style and development needs. These two factors will provide a solid foundation...prefer a more competitive style to spur you to succeed or you might enjoy working closely with others ...

  11. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...transcription >1,000-fold when activated. Lag time The time needed to induce a given promoter varies. Promoters that...inducer will have higher lag time due to the time needed for transcription/translation. Many thanks to ...

  12. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...like the right approach for your experiment, you’ll need to decide exactly which type of virus to use. Types... viral particles inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses...

  13. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... price. With this increased complexity comes the need (and/or opportunities) to modify the device for ...components are adapted and combined with creativity and feedback from the community to create state-of-the-art ...

  14. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...that there was a prior version of this plasmid. Indeed the first version is the 15th most popular plasmid...packaging, reverse transcription, and integration needed for the production of lentiviral particles. It’...

  15. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...most requested plasmids into lentiviral preps to speed up COVID-19 research even further. The following...different patterns of expression, researchers often need to determine these transduction patterns empirically...

  16. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...you would be oversized even before considering the need for some manner of polyA. In this scenario, you ... labeling at various doses.  As always, you will need to discuss injection volumes with your IACUC committee...

  17. Hot Plasmids - February 2022

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein...

  18. Clear and Accessible Writing

    Type
    Blog Post
    ...writing applied to alt text. We also were aware of the need for clear, accessible writing in scientific writing...are blurred after centrifugation and the syringe needle is inserted bevel-up at the interface between the...

  19. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...similar to the widely used FRET, but it eliminates the need for an excitation light source. Instead, a luciferase...Saito, Kenta, et al. “Luminescent proteins for high-speed single-cell and whole-body imaging.” Nat Commun....

  20. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...transfection drawbacks: transient expression and needing to work with a transfectable cell line. 2) They... process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression...
Showing: 461 - 480 of 825 results