Addgene

We narrowed to 703 results for: ESC

Showing: 461 - 480 of 703 results

Results per page:

Plasmids 101: Gram Negative and Positive Bacteria

Type

Blog Post

...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...
Using CRISPR/Cas9 to Edit Disease Out of the Genome

Type

Blog Post

...mutation in a gene that causes cataracts could be rescued by coinjection into zygotes of Cas9 mRNA and a ...
Revamp Your Lab Meetings With Creative Virtual Collaboration

Type

Blog Post

...pivotal in writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens ...
Multiplexed Overexpression of Regulatory Factors (MORF) collection

Type

Blog Post

...comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual...
Four Factors that Differentiate the Stem Cell Field

Type

Blog Post

...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...
Resume Writing for Non-academic Science Careers

Type

Blog Post

... with statistical analysis. If you have done fluorescent imaging, then you probably have experience using...
Plasmids 101: Inducible Promoters

Type

Blog Post

...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ...
Multiplex Genome Editing with CRISPR-Cpf1

Type

Blog Post

...editing! A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing. ...
Screening for Successful Genome Editing with Digital PCR

Type

Blog Post

... the exact nature of your mutations. A great description of the plethora of available sequencing options...
Top Requested Lentivirus and AAV of 2016

Type

Blog Post

...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue...
Building and Validating Recombinant Antibodies: A Case Study

Type

Blog Post

... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...
Antibodies 101: Buffers, Storage, and Conjugates

Type

Blog Post

...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...
GPCRs: How Do They Work and How Do We Study Them?

Type

Blog Post

...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes...
Viral Vectors 101: Types of viruses

Type

Blog Post

...cells only, so is not suitable for infection of quiescent cells or infrequently cycling cells but is fine...
Viral Vectors 101: The AAV Retrograde Serotype

Type

Blog Post

...trillion connections (synapses.) In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it...
How Addgene Creates Content

Type

Blog Post

...Science careers Plasmids CRISPR Viral vectors Fluorescent proteins ...
Flow Cytometry Readouts: Yes, No, and Everything in Between

Type

Blog Post

...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...worries, I've summarized the three strategies described in this post in the graphic below - including ...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...

Showing: 461 - 480 of 703 results

Results per page:

Plasmids 101: Gram Negative and Positive Bacteria

Using CRISPR/Cas9 to Edit Disease Out of the Genome

Revamp Your Lab Meetings With Creative Virtual Collaboration

Multiplexed Overexpression of Regulatory Factors (MORF) collection

Four Factors that Differentiate the Stem Cell Field

Resume Writing for Non-academic Science Careers

Plasmids 101: Inducible Promoters

Multiplex Genome Editing with CRISPR-Cpf1

Screening for Successful Genome Editing with Digital PCR

Top Requested Lentivirus and AAV of 2016

Building and Validating Recombinant Antibodies: A Case Study

Antibodies 101: Buffers, Storage, and Conjugates

GPCRs: How Do They Work and How Do We Study Them?

CasPEDIA: A Functional Classification of Cas Enzymes

Viral Vectors 101: Types of viruses

Viral Vectors 101: The AAV Retrograde Serotype

How Addgene Creates Content

Flow Cytometry Readouts: Yes, No, and Everything in Between

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

CRISPR 101: Non-Homologous End Joining

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections