We narrowed to 711 results for: ESC

1. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   ...all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using...

2. With Our New Viral Service, We're Taking CRISPR Further!

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   Blog Post

   ...and host our expression data (western blots or fluorescent microscopy) on the material pages associated ...

3. Learning Technical Skills at a Lab Central Workshop

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   Blog Post

   ...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

4. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   Blog Post

   ...comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual...

5. Revamp Your Lab Meetings With Creative Virtual Collaboration

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   Blog Post

   ...pivotal in writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens ...

6. What Good Citizenship Can Do for Reproducibility in Science

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   Blog Post

   ... board. Eventually, you get lucky and manage to escape, but you’ve lost a lot of time. It doesn’t have...

7. Plasmids 101: Gram Negative and Positive Bacteria

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   Blog Post

   ...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...

8. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   Blog Post

   ...mutation in a gene that causes cataracts could be rescued by coinjection into zygotes of Cas9 mRNA and a ...

9. Four Factors that Differentiate the Stem Cell Field

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   Blog Post

   ...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...

10. Resume Writing for Non-academic Science Careers

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    Blog Post

    ... with statistical analysis. If you have done fluorescent imaging, then you probably have experience using...

11. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    Blog Post

    ...can quantify shifts in signal using the mean fluorescence intensity (MFI) of the different populations...

12. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

    ...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...

13. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ... the exact nature of your mutations. A great description of the plethora of available sequencing options...

14. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ...anti-mCherry antibody rather than endogenous mCherry fluorescence when visualizing hM4D(Gi)-mCherry in fixed tissue...

15. Plasmids 101: Inducible Promoters

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    Blog Post

    ...induction of a promoter. Below, you’ll find a short description of these three types of inducible promoters, ...

16. Viral Vectors 101: Types of viruses

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    Blog Post

    ...cells only, so is not suitable for infection of quiescent cells or infrequently cycling cells but is fine...

17. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post

    ...trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg) and how it...

18. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post

    ... moved to direct functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method...

19. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    ...Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that can be activated to emit light...

20. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post

    ...be used to detect G protein activation via bioluminescence resonance energy transfer 2 (BRET2). Check ...