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We narrowed to 527 results for: HA tag

Showing: 461 - 480 of 527 results

  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...since its development, CRISPR/Cas9 genome editing has been used to study the effect of gene knockout in...-target effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular...leading nucleotide could be processed, a distinct advantage over other methods that favor certain nucleotides...potential of PTGs. Applications of PTGs The major advantage of the PTG system is its universality; robust ...

  2. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...Collin’s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and ...of technologies, effectively communicating the advantages embodied, and driving the enterprise value of...plan competition and advance investors to the next stage of diligence." In business competitions everything... be OK with the constant fear of failure at all stages in the startup". To gain insight into "the random-walk...

  4. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...PAM motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform...lab discovered a new Cas protein, CasX, from metagenomic analysis of microbial DNA from groundwater. Now...

  5. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...with chemicals in it,” leaving me to wonder how he has survived this long without downing any dihydrogen...graduate with my BSc in Anatomy, the University of Otago had established the Centre for Science communication...Department of Science Communication at the University of Otago. Her research focuses on misinformation about sexual...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...AAV for your experiment. The Addgene viral service has now expanded to offer many of our control plasmids...tools for light mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control...recombination of lox sites. This system takes advantage of the extensive supply of transgenic reporter...

  7. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...titers found in our in-stock preps. If a request has technical considerations that may affect expected...specialist will reach out to you during the request stage, before you order.  Timeline Once your order is ...

  8. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...to a variety of careers outside of academia. She has had previous graduate students and postdocs go on...interviewed Addgene depositors who are at various stages in their careers to get advice on choosing a lab...

  9. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...amplification are endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity...elution of plasmid DNA through their kit. The advantage of using a Tris buffer is that it allows better...

  10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...logical pattern across the poster. This style has the advantage of being different and potentially more catchy...to your audience. With a poster, you have the advantage of being able to verbally communicate your work...and acknowledgements sections are inserted. The advantage of this style is that it will display your data...

  11. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector alone... to create engineered mouse models at embryonic stages, and the pros and cons of different genome editing...mice are engineered during the embryonic stem cell stage using one of several techniques. The longest standing...

  12. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...platform. During the conference If the conference has a social media policy, this should be on their website...conference hashtag to set one up. It’s also helpful to write your Twitter handle on your nametag for the ...Check out the conference website for any relevant hashtags and handles to help you find other scientists ... share their research on social media. Use the hashtag if you are live-tweeting any sessions that the ...

  13. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one, many viral...Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific sensors designed...

  15. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...In these instances, the cloning vector typically has two separate resistance cassettes and your gene of...Additionally, the presence of a plasmid is disadvantageous from the bacterium's perspective – a plasmid-containing...

  16. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ..., another method to create point mutations, also has a strict editing window about 15 nt upstream of the...NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting domain. However, these alternative...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition. ...cell population measured with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses...

  19. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...writing, etc. Also, it is unlikely that a mentor has the only relevant expertise and perspective for your...been doing something all wrong or that a reagent has not been convincingly demonstrated to work for your... be needed to achieve your intended next career stage and create a timeline for achieving these goals....

  20. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...for aerosol contamination. As most centrifugation has to be done outside of a biosafety cabinet, you’ll... organizations or cities may require a higher percentage of the decontaminate to be used for BSL-2 waste...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate...
Showing: 461 - 480 of 527 results