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We narrowed to 934 results for: INA

Showing: 461 - 480 of 934 results

  1. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post
    ...antimicrobial.  Conjugates And HRP brings us handily to the final topic of this post, the conjugates, or signaling... a brief introduction to the wide, wild, and fascinating field of signaling molecules. Assay Type Westerns..., simple to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics: Antibodies...

  2. Antibodies 101: Isotypes

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    Blog Post
    ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...switching is caused by a specialized type of DNA recombination. Heavy chain constant genes are clustered together... Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. B cells start by...heavy-chain constant domain gene cluster. DNA recombination can delete intervening heavy-chain constant ...

  3. Antibodies 101: Monoclonal Antibodies

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    Blog Post
    ...continue to produce the specific antibody that the original B cell produced. Because of this, all of the antibodies...hybridomas producing the most specific antibodies. Finally, the selected hybridoma is expanded as a clonal...reproducible experimental results. When you are originally selecting a hybridoma, you can choose one that...from the hybridoma into a plasmid to create a recombinant antibody.     Figure 2: Changes to the...

  4. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...the page. Finally, as previously mentioned, enlist the help of your network to polish the final draft prior...don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...notice within 24 hrs, send a note to the program coordinator.   6. Virtual interview prep Prepare for your...

  5. Viral Vectors 101: Viral Vector Elements

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    Blog Post
    ...sides of this promoter-gene combo are two long terminal repeats (LTRs). These LTRs define the limit of...respectively, act as RNA polymerase II promoter and terminator regions and are slightly different in sequence...expression constructs. Sandwiched between two inverted terminal repeats (ITRs) is the promoter and cargo, in that... it to evade detection by the host. Of note, recombinant adenovirus is missing the endogenous E1 gene,...

  6. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post
    ...backbone with a T7 promoter driving multiple combinations of WT, codon optimized, untagged, cleavage sites... sites (thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include...found in the bacterial expression plasmids. Each combination is present in two sets of backbones which drive...expression system. Mammalian expression plasmids Finally a set of wild type and codon optimized, full length...

  7. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post
    ...Inflammation and Immunity, as well as various combinations thereof (so called “Immuno-Oncology”) is exploding...tackle the challenge of curing cancer in cross-disciplinary projects and collaborations. Summarized by Professor...the boost it needs to help rid us of cancer in combination with chemotherapy. To dive deeper into this field...exciting development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor...

  8. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...well as an efficient mechanism of homologous recombination to facilitate simple gene replacement/mutation...directly into the host chromosome via homologous recombination. Yeast Replicating plasmids (YRp):  These vectors...some antibiotics. Auxotrophy is defined as the inability of an organism to synthesize a particular organic...selection element pairs must be utilized or a new combination needs to be created in advance of the experiment...

  9. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...convenient site for conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the...If so, you are not alone. Full-sized antibodies dominate the world of research affinity reagents, and for...not a “Fab” fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide...

  10. Antibodies 101: Secondary Antibodies

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    Blog Post
    ...secondary will bind to all the primary antibodies indiscriminately, giving them the same output signal. In these... Time is a precious commodity in the lab and eliminating the need for secondary antibodies would save ...use a primary antibody labelled with a probe, eliminating the need for a secondary antibody. Other immunohistochemistry... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...

  11. A Look at Addgene's QC Process

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    Blog Post
    ... something completely different than what was originally intended. Sharing unverified plasmids can lead... complete plasmid sequencing using an in-house Illumina MiSeq. The raw sequencing reads are assembled ...particularly difficult to sequence and assemble using the Illumina MiSeq platform, such as GC-rich regions and highly...short 200–250 base pair reads sequenced on the Illumina MiSeq.  Resolving QC issues If the QC team identifies...

  13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post
    ...temperature, humidity, noise level, circadian effects (inactive versus active phase) Experimenter Interaction... of certain genes. A popular method uses Cre recombinase on transgenic flox animals to introduce modifications...- from basic types like locomotion and motor coordination to more complex situations such as learning ...Sena-Esteves M. Gene transfer to the CNS using recombinant adeno-associated virus. Curr Protoc Microbiol...

  14. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...post was originally written by Tyler Ford in March 2017 and updated by Angela Holmes and Christina Mork in..., spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High or Low Copy... with highly repetitive sequences (prone to recombination in bacteria) such as lentiviral, retroviral,...

  15. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ... proceeding to ultracentrifugation to avoid contaminating your transduction with the packaging cells. ...may be the result of plasmid integration via recombination during transfection, and does not necessarily...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...., Michael J. Yezzi, and Barbara R. Young. "Recombination events during integration of transfected DNA...

  16. Degrading DNA with Cascade-Cas3

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    Blog Post
    ...system. Most Cascade complexes are comprised of a combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Type I-...a fisherman would reel in a big catch. It was originally suspected that Cas3 moved a couple of kilobases...understood but likely occurs in similar regions. This combination of chewing up one stand and cutting the template...mutations if the chromosome is repaired with all the original DNA. With Cas3, you don’t have to play these odds...

  17. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post
    ...(the precise mechanism isn’t well understood). Finally, TOPO cloning is another good option to consider...integration sequences, relies on site-specific recombination reactions catalyzed by BP Clonase and LR Clonase... to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The ligation-independent...since one can pool the components and define their final order through sequence homology. Similarly, Golden...

  18. PhD Applications After COVID

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    Blog Post
    ... program is the right fit for you without the financial and logistical stress that accompanies traveling...faculty, students, and each other. Before COVID-19, financial and logistical obstacles could make touring potential...COVID-19? How are students funded? Are there any financial aspects that are confusing? During a traditional...interviews for a variety of reasons (logistical, financial, etc…) now have options to fully engage in the...

  19. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post
    ... interest from its production site to its final destination. As an example, most secreted proteins are...pathway to their subcellular or extracellular destination. At the end of their life cycle, proteins may...through the vesicles to the lysosome where they are finally degraded (2, 3). Once you've created your fusion...

  20. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...expressed, they predominantly fluoresce one color but slowly mature until they predominantly fluoresce a ...of protein fusions or expressed independently) originally fluoresce green or blue and, when they mature...hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of mature to immature...
Showing: 461 - 480 of 934 results