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We narrowed to 917 results for: IRE;

Showing: 461 - 480 of 917 results

  1. Finding Your Perfect Job After University

    Type
    Blog Post
    ... degree. I loved parasitology. However, when I enquired about roles in this field, most jobs needed at...understood why parasitology roles in the field require experience. Working in industry: Microbiology ...redundancies can be made within the company; new staff hired in anticipation of the contract suddenly have less...institutes. The lab at the University of Queensland required a scientist for a 6 month project to validate ...

  2. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ... to stain all DNA. Some amount of expertise is required in this process because result interpretation ...serum to be used for subculturing. Avoid using expired products or reagents that have been stored for ...cross-contamination. Make sure you keep all the required equipment and reagents inside the laminar airflow... Biotechnology.       References Degeling MH, Maguire CA, Bovenberg MSS, Tannous BA (2012) Sensitive ...

  3. Typing CRISPR Systems

    Type
    Blog Post
    ...systems has led to extreme sequence divergence that requires a multi-pronged analysis to sort effectively. ...efficiency when conducting edits using homology-directed repair. There are 10 subtypes of type V, named...select RNA transcripts, or be engineered to conduct direct RNA editing. There are four subtypes, A–D, with...surface-level function of each type could lead you to new directions in your research. We all have our preferences...

  4. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ... rabies materials may be required for some countries and may not be required for others. You will be notified... notified if an export license is not required for your country after checkout, and the processing fee...

  5. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    ...University. Effective science communication is required to promote public support for research and to ...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

  6. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ...after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation?  Great... we decided to include 10 plasmids that can be directly used in the research on the SARS-CoV-2. Nine of...experiments such as cloning, digestions, PCR etc. and/or directly long term storage. Every preparation is quality...

  7. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    ...The Developmental Studies Hybridoma Bank (DSHB), directed by David R. Soll, is a repository for monoclonal...most popular monoclonal antibodies include those directed against human transcription factors such as PAX7...to Our Guest Blogger!   David Soll has been the Director of the Developmental Studies Hybridoma Bank (DSHB...

  8. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    ... taking up a position on the Addgene Board of Directors. We're excited to watch both Addgene and Sample...How to Be A Good Manager from Addgene Executive Director Joanne Kamens Resources on the Addgene Website... Check Out the Other Members of our Board of Directors Become a Part of the Addgene Community ...

  9. Science Communication for Everyone

    Type
    Blog Post
    ...every science field as we expand and encourage more direct conversations between scientists and non-scientists...process of starting a forum where non-scientists can directly approach scientists and doctors with their questions.... Use hashtags effectively. This would help you direct your content to the right audience. Avoid using...

  10. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription factors...RNA. As a result, targeting the RNA of interest requires that the RNA be tagged. “This tool makes it possible...She also notes that for reasons that are not yet entirely clear some PUF proteins lose their solubility....

  11. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...) that can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR). ...CIB1 fusion binds to the target DNA sequence as directed by the guide RNA (gRNA), while the CRY2PHR-activator...employed in other split systems where light is required to reconstitute enzymatic activity. Photoactivatable...

  12. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...information to make improvements. Members connect directly with one another – not just with the team leader...'t work to get people sharing.   Frequently ask direct questions for specific feedback in non-public settings...Scientists. Subscribe to the Addgene Blog to follow the entire series as it is published. For more reading try...

  13. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...work, determination, passion, and patience are required to build and lead a productive and successful ...sharing meals, going on trips, and going to New Hampshire in the summer for the annual lab retreat. How ...no play Although hard work and perseverance are required to achieve success in the lab, it is important...

  14. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...to dive into conversations with other users. The entire dataset can be downloaded from Zenodo, including... the Reagent Resources table or by downloading directly from the Zenodo dataset.     Figure 2... and answer questions. Andrea and Ziv were inspired by the open-software community when designing ...

  15. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ... in the body, like the gastrointestinal tract. Require Administration of Substrate: Some luciferase reporters... such as Renilla luciferase (RLuc) or NanoLuc, require injection of substrate to produce bioluminescence...arg2 vesicles is the amount of soundwave pressure required to collapse them: arg2 vesicles collapse at a ...

  16. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ... Matchmaker Day 2: Homology directed repair: CRISPR 101: Homology Directed Repair and 3 Tips to Improve...101: Multiplex Expression of gRNAs and The PAM Requirement and Expanding CRISPR Beyond SpCas9 Day 8: Repair...

  18. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...    Novel fluorescent enzyme biosensors provide direct, specific and sensitive detection of the plant ...strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers’s lab has designed...than on the fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors ...

  19. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...transgene (Lin et al., 2018). However, this system requires precise titration of the AAV. So far, tools to...to overcome this challenge in Drosophila require heat-shocking the system or using chemical inducers of... that will be labeled in a given population is directly correlated to the length of the truncated attP...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... the main source of the body’s DA. Neurons often fire at a certain frequency, and this frequency can indicate...frequency of its release) are well understood, the direct effect of DA binding to a target neuron is more...the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes...
Showing: 461 - 480 of 917 results