...after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation?  Great... we decided to include 10 plasmids that can be directly used in the research on the SARS-CoV-2. Nine of...experiments such as cloning, digestions, PCR etc. and/or directly long term storage. Every preparation is quality...

...The Developmental Studies Hybridoma Bank (DSHB), directed by David R. Soll, is a repository for monoclonal...most popular monoclonal antibodies include those directed against human transcription factors such as PAX7...to Our Guest Blogger!   David Soll has been the Director of the Developmental Studies Hybridoma Bank (DSHB...

... taking up a position on the Addgene Board of Directors. We're excited to watch both Addgene and Sample...How to Be A Good Manager from Addgene Executive Director Joanne Kamens Resources on the Addgene Website... Check Out the Other Members of our Board of Directors Become a Part of the Addgene Community ...

...every science field as we expand and encourage more direct conversations between scientists and non-scientists...process of starting a forum where non-scientists can directly approach scientists and doctors with their questions.... Use hashtags effectively. This would help you direct your content to the right audience. Avoid using...

...meteoric rise of CRISPR technology, the ability to direct enzymes – from nucleases to transcription factors...RNA. As a result, targeting the RNA of interest requires that the RNA be tagged. “This tool makes it possible...She also notes that for reasons that are not yet entirely clear some PUF proteins lose their solubility....

...information to make improvements. Members connect directly with one another – not just with the team leader...'t work to get people sharing.   Frequently ask direct questions for specific feedback in non-public settings...Scientists. Subscribe to the Addgene Blog to follow the entire series as it is published. For more reading try...

...) that can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR). ...CIB1 fusion binds to the target DNA sequence as directed by the guide RNA (gRNA), while the CRY2PHR-activator...employed in other split systems where light is required to reconstitute enzymatic activity. Photoactivatable...

...work, determination, passion, and patience are required to build and lead a productive and successful ...sharing meals, going on trips, and going to New Hampshire in the summer for the annual lab retreat. How ...no play Although hard work and perseverance are required to achieve success in the lab, it is important...

...to dive into conversations with other users. The entire dataset can be downloaded from Zenodo, including... the Reagent Resources table or by downloading directly from the Zenodo dataset.     Figure 2... and answer questions. Andrea and Ziv were inspired by the open-software community when designing ...

... in the body, like the gastrointestinal tract. Require Administration of Substrate: Some luciferase reporters... such as Renilla luciferase (RLuc) or NanoLuc, require injection of substrate to produce bioluminescence...arg2 vesicles is the amount of soundwave pressure required to collapse them: arg2 vesicles collapse at a ...

...transgene (Lin et al., 2018). However, this system requires precise titration of the AAV. So far, tools to...to overcome this challenge in Drosophila require heat-shocking the system or using chemical inducers of... that will be labeled in a given population is directly correlated to the length of the truncated attP...

... the main source of the body’s DA. Neurons often fire at a certain frequency, and this frequency can indicate...frequency of its release) are well understood, the direct effect of DA binding to a target neuron is more...the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes...

...are under a Creative Commons 3.0 license, which requires users to give appropriate credit, provide a link...indicate if changes were made to the images. This requirement means the images are probably better suited for... image search is a great tool for searching the entire internet for scientific graphics. The tools settings...

...    Novel fluorescent enzyme biosensors provide direct, specific and sensitive detection of the plant ...strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers’s lab has designed...than on the fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors ...

... Matchmaker Day 2: Homology directed repair: CRISPR 101: Homology Directed Repair and 3 Tips to Improve...101: Multiplex Expression of gRNAs and The PAM Requirement and Expanding CRISPR Beyond SpCas9 Day 8: Repair...

...Caitlin was hired, she was promoted to Product Development Manager and now manages the entire curriculum...about kit cost and safety.  Being a manager also requires Caitlin to look at the big picture: “It’s been...

...Running4Rare are paired with patient partners so that they can help tell their stories. I was paired with a six-year-old...where my wife Lianna has made her career as Senior Director of Biology, is helping us fight against FOP in...

...many roles to play inside, on the outskirts, and entirely outside of academia. These roles can include positions...position you’ll be happy in once you’ve finally acquired that PhD. Resources at Addgene Read Other Careers...Management for Scienitsts from Addgene Executive Director Joanne Kamens ...

...cells by any means, but are unique in that they require delivery to be viral-mediated. Transformations ...retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector...weeks, depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression...

... Growing Arabidopsis in the classroom does not require a huge investment in resources. The materials needed...guideline, experiments that include growing plants require pots, trays, soil, fertilizer, seeds and a growth...space with fluorescent lights. Other experiments require only seeds, petri dishes, and filter paper. Another...