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We narrowed to 1,034 results for: LAS

Showing: 461 - 480 of 1034 results

  1. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...you the laser configuration. In general, if two fluorescent markers are read by the same laser, they will... markers you intend to use and your cytometer’s laser configuration. Your flow core or an online manual...reads out the fluorophores FITC and PE on the same laser, you will need to compensate between the two of ...your flow cytometer reads them out on different lasers, you should not need to compensate between them...

  2. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...antibiotic selectable markers by the destination plasmid. Antibiotic selection allowed for high throughput...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...'s work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...cells. FPs classified by the emission color (emission wavelength range) FPs are usually classified by emission... relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other FPs have been...emission. For example, if your microscope has only two lasers, at 488nm and 561nm, you will not be able to use... comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) Three Review...

  4. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...outlets have addressed CRISPR frequently over the last several years, but lay readers may be less familiar...is being done has changed significantly over the last decade. He describes the detection of gravitational...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...competitiveness of U.S. science and technology.” This last section of the book, Future Science, describes the...research. Even if Zimmer was able to rewrite the last chapter of his book today--the day you are reading...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (UGI) minimizes...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ...smaller vectors suitable for transient assays using protoplast transformation. Cytosine base editors for plant...

  6. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...cells; however, Mycoplasma species do not possess cell walls. As a result, Mycoplasma are intrinsically...resistance are resistant to three or more different classes, or types, of drugs used to treat them. Very often...fantastic example of intrinsic resistance would be Mycoplasma species’ resistance to beta-lactam antibiotics...intrinsically resistant to this class of antibiotic. Acquired resistance can be the result of one bacterium sharing...

  7. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ... either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific cell type. The Confetti...et al., 2011) labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage...a specific tissue. XFPs are introduced using a plasmid construct called Zebrabow-GateDest containing an...introduced in vivo either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed...

  8. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...photostability of the Corn-DFHO complex. For dessert (last but not least), the only fruit-named aptamer, Mango...

  9. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections...finger nucleases to remove exon 51 in DMD patient myoblasts. They observed a 13% removal rate of exon 51, ...gRNAs to delete exon 51 or exons 45-55 in patient myoblasts; when injected into DMD mice, these cells expressed... to ascertain how long the rescue phenotype will last, and more importantly, if it does extend lifespan... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...

  10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    ...recombinant antibodies (rAbs), which are produced from plasmids and have very little batch-to-batch variation.... of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal, recombinant... buffer than proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing...However, these systems are expensive, generate plastic waste, and limit the amount of optimization that...temperature for 1–2 years and are available in formulas specific to your choice of detection methods. ...antibody required. Reporters There are two main classes of reporters: chemiluminescence enzymes and fluorescent...

  13. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...organization (now with more than 50% international plasmid distribution!) Chapter 8 - Opportunities to create...

  14. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...can request any of over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged in your...Request With Packaged on Request, you can find your plasmids and your viral packaging service in the same place...packaged on request process, simply navigate to your plasmid of interest. If it’s eligible for packaged on request...special processes involved; simply request your plasmid of interest in your desired serotype, place your...

  15. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...Blog Learn New Plasmid Cloning Techniques Pushing the Limits of DNA Assembly Plasmid Cloning by PCR ...including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation...resistance-mediating polymorphisms in African samples of Plasmodium falciparum." Journal of clinical microbiology...Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "Last stop on the road to repair: structure of E. coli...

  16. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... there? A typical biomedical postdoc fellowship lasts five years. Most universities and institutes annually...feel like they've made enough progress since the last presentation. Yet, PFs need to tell and sell their... practice. If you simply recycle slides from the last time you gave a presentation to a specific group...trainees, communicate your expectations, and teach classes. Therefore, seek out management and conflict resolution...quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins...

  17. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...Last week, 20 students and four chaperones from Denis-Yarmouth Regional High School visited our labs ...some of the teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very...real working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite part... all over the world know that we can help with plasmid sharing! Judging by how many prizes were given ...

  18. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...exhibitor booth in order to help scientists find the plasmids they need for their research, show labs how they...about all the amazing research being done with plasmids. I heard an impressive talk about the use of optogenetics...and resources by storing and distributing their plasmids with the scientific community. All of this speaks... meet our Addgenie crew and sign-up for a free plasmid! Also check out the PLOS Neuroscience Community...

  19. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...s incredible.” Looking for CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References...important advance was quickly followed by even splashier news: Jiahao Sha of Nanjing Medical University...understanding of how CRISPR/Cas9s actually work. Last month, the Lawrence Berkeley Lab's Jennifer Doudna...

  20. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    ...Last updated Apr 17, 2020. Before being adapted by scientists to edit the genome of virtually any organisms...size of other Cas proteins found in the so-called class 2 CRISPR-Cas systems. There are 24 variants of the...regions containing the putative tracrRNA into a plasmid and expressed it in an E. coli bacteria strain....sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR guide page Browse the...
Showing: 461 - 480 of 1034 results