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We narrowed to 1,038 results for: LAS

Showing: 461 - 480 of 1038 results

  1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...sometimes requires intracellular injections into the cytoplasm or pronucleus of the zygote which is both technically...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

  2. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...in research can fall under one of two broad classifications: inbred or outbred. Inbred strains share a ...embryonic stem cells that are then injected into a blastocyst. There are several types of genetically engineered...Modified embryonic stem cells are injected into a blastocyst which is then implanted into a female animal ...embryonic stem cells, which are then injected into a blastocyst. Nucleases can also be used to engineer knockout...create mosaic animals where not all cells in the blastocyst were successfully edited by the injection of ...

  3. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare diseases ...Richard Gronostajski who each are being awarded free plasmids form Addgene to accelerate their research. Read... on to learn how these researchers plan to use plasmids from Addgene to advance their research on diseases...Prof. Videira and collaborators will use Addgene plasmids to immortalize CDG patient-derived immune cell...Nfix-negative neural stem cells. The lab will use plasmids from Addgene to construct NFIX expression vectors...

  4. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...antibiotic selectable markers by the destination plasmid. Antibiotic selection allowed for high throughput...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...'s work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...

  5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...transcription from the accessory plasmid (AP) encoding phage gene III. The accessory plasmid library contained all...PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...

  6. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...you the laser configuration. In general, if two fluorescent markers are read by the same laser, they will... markers you intend to use and your cytometer’s laser configuration. Your flow core or an online manual...reads out the fluorophores FITC and PE on the same laser, you will need to compensate between the two of ...your flow cytometer reads them out on different lasers, you should not need to compensate between them...

  7. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ... in the laboratory became frustrated with using glass cloning rings to isolate colonies of antibiotic-...growing cells we sometimes use TPP peelaway T-75 flasks to provide a greater surface area (the amount of...contamination. We do not normally use antibiotics. Lastly, and perhaps obviously, it is important to have...Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...

  8. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...JEDI, and many more. They can be targeted to the plasma membrane to measure membrane potential and can ...containing GEVIs. Chemigenetic sensors are better classified by their fluorescent reporter and the mechanism...fused to a single fluorescent protein (FP). This class of GEVI was the earliest developed voltage sensor...these sensors contained a FP that localized to the plasma membrane due to the addition of several targeting...lipophilic compound that distributes itself within the plasma membrane as well. Another flavor of these tools...

  9. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...cells. FPs classified by the emission color (emission wavelength range) FPs are usually classified by emission... relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other FPs have been...emission. For example, if your microscope has only two lasers, at 488nm and 561nm, you will not be able to use... comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) Three Review...

  10. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...outlets have addressed CRISPR frequently over the last several years, but lay readers may be less familiar...is being done has changed significantly over the last decade. He describes the detection of gravitational...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...competitiveness of U.S. science and technology.” This last section of the book, Future Science, describes the...research. Even if Zimmer was able to rewrite the last chapter of his book today--the day you are reading...

  11. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (UGI) minimizes...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ...smaller vectors suitable for transient assays using protoplast transformation. Cytosine base editors for plant...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...cells; however, Mycoplasma species do not possess cell walls. As a result, Mycoplasma are intrinsically...resistance are resistant to three or more different classes, or types, of drugs used to treat them. Very often...fantastic example of intrinsic resistance would be Mycoplasma species’ resistance to beta-lactam antibiotics...intrinsically resistant to this class of antibiotic. Acquired resistance can be the result of one bacterium sharing...

  13. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ... either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific cell type. The Confetti...et al., 2011) labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage...a specific tissue. XFPs are introduced using a plasmid construct called Zebrabow-GateDest containing an...introduced in vivo either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed...

  14. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...photostability of the Corn-DFHO complex. For dessert (last but not least), the only fruit-named aptamer, Mango...

  15. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections...finger nucleases to remove exon 51 in DMD patient myoblasts. They observed a 13% removal rate of exon 51, ...gRNAs to delete exon 51 or exons 45-55 in patient myoblasts; when injected into DMD mice, these cells expressed... to ascertain how long the rescue phenotype will last, and more importantly, if it does extend lifespan... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...

  16. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...

  17. Technical Design of a Western Blot

    Type
    Blog Post
    ...recombinant antibodies (rAbs), which are produced from plasmids and have very little batch-to-batch variation.... of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal, recombinant... buffer than proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing...However, these systems are expensive, generate plastic waste, and limit the amount of optimization that...temperature for 1–2 years and are available in formulas specific to your choice of detection methods. ...antibody required. Reporters There are two main classes of reporters: chemiluminescence enzymes and fluorescent...

  19. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...organization (now with more than 50% international plasmid distribution!) Chapter 8 - Opportunities to create...

  20. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...can request any of over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged in your...Request With Packaged on Request, you can find your plasmids and your viral packaging service in the same place...packaged on request process, simply navigate to your plasmid of interest. If it’s eligible for packaged on request...special processes involved; simply request your plasmid of interest in your desired serotype, place your...
Showing: 461 - 480 of 1038 results