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Showing: 461 - 480 of 811 results

  3. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

  4. Fostering communication in remote collaborations

    Type
    Blog Post
    ...and improves overall communication.  Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of...

  6. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1. In SunTag nomenclature throughout...Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org ...

  9. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not...of viral vectors (which many researchers will informally refer to as viruses) are named after the viruses...

  10. What the HEK?

    Type
    Blog Post
    ... is present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and...

  13. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

  18. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...genomic drift or limited material from polyclonal animal preparations (Bradbury et al., 2015). All of Addgene...were tested for specificity in knock-out lines or animals.  Addgene will continue to add more antibodies,...

  19. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...valuable for CRISPR/Cas9 stem cell editing. The small molecule NSDB and Na+ hypertonicity are key elements... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...

  20. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important elements...enzymes cut at known sites within the ITR. However, small deletions and point mutations will go undetected...
Showing: 461 - 480 of 811 results