We narrowed to 817 results for: MAL

1. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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   Blog Post

   ...it’s a probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years... for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed PMID: 29300010...

2. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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   ...occurs infrequently and rarely makes it into the small selection of data found in full-length publications...experimental results but, obviously, this is not optimal. What if you could save the figures of interest...

3. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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   Blog Post

   ...downloadable in a small file size with a Biorender watermark, which is usually ok for informal settings like...

4. Understanding GPCR Signaling Using TRUPATH

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   ...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   ...the same in both cases, but activation depends on small molecule activation (chemogenetics) or light (optogenetics.... TL;DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics...

6. Fostering communication in remote collaborations

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   Blog Post

   ...and improves overall communication.  Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of...

7. Fluorescent Proteins 101: Fluorescent Biosensors

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   ...activity. Small molecule biosensors Addgene has many sensors that measure the concentration of small molecules...

8. SunTag and Fluorescent Imaging

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   ...reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1. In SunTag nomenclature throughout...Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org ...

9. CRISPR 101: Drug discovery and CRISPR

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   .... – CRISPR can be used to generate cell line or animal models which have the precise disease genetics.... drug selectively kills the wildtype line with minimal to no toxicity in the knockout line, it is likely...

10. Plasmids 101: Gene, enhancer, and promoter trapping

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    Blog Post

    ... reporter gene flanked by a minimal promoter which can integrate proximal to an endogenous gene to turn...

11. Cellosaurus: A Cell Line Information Database

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    Blog Post

    ... of interest. You can filter CLASTR results by minimal number of markers and similarity score match. This...allele search preferences to help you identify proximal lines you care about. If you have cell lines without...

12. Running for Rare Disease, Running for FOP, Running for AJ

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    Blog Post

    ... and “exercise” consisted of taking walks in the mall. I took action, joined a gym, and started running...scientific community know, treatments that work well in animal models do not always work as efficiently in humans...

13. Choosing a Good Mentor for Scientists

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    Blog Post

    ...scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist...

14. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    Blog Post

    ...advantages over conventional antibodies: they are small (12-15 kDa), stable, highly soluble, and non-immunogenic...to an engineered peroxidase, (APEX) that labels proximal proteins with biotin as an affinity tag. This ...

15. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post

    ...conditions of hyperosmotic stress." Advances in animal cell biology and technology for bioprocesses (1989...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." ...

16. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    Blog Post

    ...expression system within the Addgene repository is mammalian, but the largest source of unique gene sequences...frequent transfer of genes is from Proteobacteria to mammalian expression vectors. And more broadly, their data...

17. Plasmids 101: Origin of Replication

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    ... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

18. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    Blog Post

    ...to request support for our operations until our normal work resumed. Addgene distributes and fulfills ...supporting research could provide Addgene with a small amount of funds, the community as a whole could ...

19. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post

    ...as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins...

20. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    Blog Post

    ...stain to assess purity. For labs that produce a small number of AAV preparations this level of quality...expression cassette that we want, it also includes a small amount of spurious DNA from the packaging cell line...