We narrowed to 810 results for: Mal

... reporter gene flanked by a minimal promoter which can integrate proximal to an endogenous gene to turn...

... of interest. You can filter CLASTR results by minimal number of markers and similarity score match. This...allele search preferences to help you identify proximal lines you care about. If you have cell lines without...

... and “exercise” consisted of taking walks in the mall. I took action, joined a gym, and started running...scientific community know, treatments that work well in animal models do not always work as efficiently in humans...

...scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist...

...genomic drift or limited material from polyclonal animal preparations (Bradbury et al., 2015). All of Addgene...were tested for specificity in knock-out lines or animals.  Addgene will continue to add more antibodies,...

...decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not...of viral vectors (which many researchers will informally refer to as viruses) are named after the viruses...

...expression system within the Addgene repository is mammalian, but the largest source of unique gene sequences...frequent transfer of genes is from Proteobacteria to mammalian expression vectors. And more broadly, their data...

...advantages over conventional antibodies: they are small (12-15 kDa), stable, highly soluble, and non-immunogenic...to an engineered peroxidase, (APEX) that labels proximal proteins with biotin as an affinity tag. This ...

... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

...to request support for our operations until our normal work resumed. Addgene distributes and fulfills ...supporting research could provide Addgene with a small amount of funds, the community as a whole could ...

...as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins...

...stain to assess purity. For labs that produce a small number of AAV preparations this level of quality...expression cassette that we want, it also includes a small amount of spurious DNA from the packaging cell line...

... is present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and...

...conditions of hyperosmotic stress." Advances in animal cell biology and technology for bioprocesses (1989...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." ...

...valuable for CRISPR/Cas9 stem cell editing. The small molecule NSDB and Na+ hypertonicity are key elements... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...

...examining relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed...doesn’t love a book series that features a young female protagonist, some clever magic, and a satisfying...

...recently released our serotype testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged ...resting fluorescence and can be used for imaging small neuronal processes (dendrites and axons). jGCaMP7c...

...generations of iGluSnFR had limitations: they were not optimal for experiments requiring high signal to noise ...fusion to the PDGFR transmembrane domain in the mammalian expression pMinDisplay vector. GPI contains a ...

...handling recommendations Not all O-rings function optimally at -80°C. For samples that are shipped on dry ...changes in our solutions are transient and return to normal once the tube is opened and equilibrium with ambient...

...aside Breakout Rooms to divide the audience into small sessions and do a quick break to chat. This can ... but can also be used to brainstorm on data in smaller groups, where for instance the presence of the ...