We narrowed to 810 results for: Mal;

...engineering and experimental refinement to determine the optimal localization of the Rluc8 insertion within the ...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual...

...the same in both cases, but activation depends on small molecule activation (chemogenetics) or light (optogenetics.... TL;DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics...

...and improves overall communication.  Maintain informal communication In between recurring meetings, you...this casual communication reflects the type of informal communication you’d likely have with members of...

...scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist...

...decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not...of viral vectors (which many researchers will informally refer to as viruses) are named after the viruses...

... is present in this line, making it genetically female. HEKs are generally cultured as an adherent monolayer...blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and...

...conditions of hyperosmotic stress." Advances in animal cell biology and technology for bioprocesses (1989...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." ...

...expression system within the Addgene repository is mammalian, but the largest source of unique gene sequences...frequent transfer of genes is from Proteobacteria to mammalian expression vectors. And more broadly, their data...

...advantages over conventional antibodies: they are small (12-15 kDa), stable, highly soluble, and non-immunogenic...to an engineered peroxidase, (APEX) that labels proximal proteins with biotin as an affinity tag. This ...

...genomic drift or limited material from polyclonal animal preparations (Bradbury et al., 2015). All of Addgene...were tested for specificity in knock-out lines or animals.  Addgene will continue to add more antibodies,...

... from the those used to replicate the host's chromosomal DNA, but they still rely on the host machinery...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

...to request support for our operations until our normal work resumed. Addgene distributes and fulfills ...supporting research could provide Addgene with a small amount of funds, the community as a whole could ...

...as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins...

...stain to assess purity. For labs that produce a small number of AAV preparations this level of quality...expression cassette that we want, it also includes a small amount of spurious DNA from the packaging cell line...

...valuable for CRISPR/Cas9 stem cell editing. The small molecule NSDB and Na+ hypertonicity are key elements... Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles. ...

...PAM-distal side of the break and 91bp on the PAM-proximal side, consistently supported higher frequencies...attained without any of the error-prone repair that normally accompanies Cas9 cutting. We still don’t know ...

...and has extensive experience targeting RNA with small molecules. Other members’ projects range from hacking...Ph.D. grad student at Boston University studying mammalian immunology, and in his free time, he is pursuing...

...plasmids in the library are differently sized, smaller plasmids may be amplified preferentially, skewing...Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids in the library ...

... Assembly method from New England Biolabs® has minimal upfront requirements and allows you to expediently...generation, green chemistry and understanding the minimal genome. Many of these techniques require more complex...

...handling recommendations Not all O-rings function optimally at -80°C. For samples that are shipped on dry ...changes in our solutions are transient and return to normal once the tube is opened and equilibrium with ambient...