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We narrowed to 783 results for: Mos

Showing: 461 - 480 of 783 results
  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...(amino acid sequence DYKDDDDK) may be one of the most versatile. Whether you plan to purify or image a...
  2. Viral Vectors 101: Chemogenetics

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    Blog Post
    ...depending on what cell type it is expressed in. The most common target for chemogenetic manipulation are ...have been derived from muscarinic receptors. The most popular agonist for muscarinic receptor derived ...
  3. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...similar homology to the site of interest or create mosaic animals where not all cells in the blastocyst were...small molecule that regulates Cre expression. The most common drug used to induce the expression of Cre...
  4. Plasmids 101: Terminators and PolyA signals

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    Blog Post
    ...some engineered terminators come close (>95%). For most purposes, however, any common terminator will suffice...Addgene (1). Prokaryotic polyadenylation Although mostly thought of as a eukaryotic-specific process, prokaryotes...
  5. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...the target cell’s genome. Physical titer The two most common assays for physical titer are direct p24 ...choice can also impact transgene expression. The most accurate method of lentiviral titration uses qPCR...
  6. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post
    .... As the depositing scientist, you will have the most involvement with Step 1, and, while data entry can...every so often and this ensures you are using our most up-to-date version! We hope the deposit spreadsheet...
  7. Engaging with science and society at pgEd

    Type
    Blog Post
    ...well as schools, community events, churches or mosques. On occasion, we also get interviewed for print...fields of research, what often concerns them the most about research is its implications for their lives...
  8. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...://pubmed.ncbi.nlm.nih.gov/32675376/ mGold, the most photostable yellow fluorescent protein to date By... as bright to mVenus, the parent YFP, and is the most photostable YFP to date. As such, mGold is expected...
  9. Hot Plasmids - October 2020

    Type
    Blog Post
    ...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence, indicating a quick cell cycle...
  10. Plasmids 101: Degron Tags

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    Blog Post
    ...degrons, you have options! Below we will review the 4 most common types of degrons and some of the nuances ...degradation. HaloTag is also the degron tag with the most alternative functions — there are antibodies to ...
  11. Cloning Mammalian Cells with the Agarose Method

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    Blog Post
    ...cell lines, especially the endocrine cell lines we most commonly work with, require extra serum to survive... of the wells in the 48-well plate will grow up, most will eventually grow within a week. We screen the...
  12. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...
Showing: 461 - 480 of 783 results