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Addgene

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We narrowed to 765 results for: Mos

Showing: 461 - 480 of 765 results

  2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    .... As the depositing scientist, you will have the most involvement with Step 1, and, while data entry can...every so often and this ensures you are using our most up-to-date version! We hope the deposit spreadsheet...

  4. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...similar homology to the site of interest or create mosaic animals where not all cells in the blastocyst were...small molecule that regulates Cre expression. The most common drug used to induce the expression of Cre...

  5. Engaging with science and society at pgEd

    Type
    Blog Post
    ...well as schools, community events, churches or mosques. On occasion, we also get interviewed for print...fields of research, what often concerns them the most about research is its implications for their lives...

  7. Hot Plasmids - October 2020

    Type
    Blog Post
    ...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence, indicating a quick cell cycle...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...some engineered terminators come close (>95%). For most purposes, however, any common terminator will suffice...Addgene (1). Prokaryotic polyadenylation Although mostly thought of as a eukaryotic-specific process, prokaryotes...

  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...the target cell’s genome. Physical titer The two most common assays for physical titer are direct p24 ...choice can also impact transgene expression. The most accurate method of lentiviral titration uses qPCR...

  13. Pooled Library Amplifications

    Type
    Blog Post
    ... protocol. Created with BioRender.com.   The most important factor to consider is your desired library...what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere between...

  14. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...degrons, you have options! Below we will review the 4 most common types of degrons and some of the nuances ...degradation. HaloTag is also the degron tag with the most alternative functions – there are antibodies to ...

  15. PhD Applications After COVID

    Type
    Blog Post
    ...process of even applying to one of these programs is almost as daunting as the program itself. Picking a mentor...idea to email around three professors per program. Most applications will recommend that you name two professors...

  16. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase is thus a heat ...presence, size, and relative quantity. End point PCR is most commonly used in molecular cloning, sequencing, ...

  19. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...
Showing: 461 - 480 of 765 results