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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 772 results for: Mos

Showing: 461 - 480 of 772 results

  6. Hot Plasmids February 2024

    Type
    Blog Post
    ...in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters...(amino acid sequence DYKDDDDK) may be one of the most versatile. Whether you plan to purify or image a...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...depending on what cell type it is expressed in. The most common target for chemogenetic manipulation are ...have been derived from muscarinic receptors. The most popular agonist for muscarinic receptor derived ...

  8. A Look at Addgene's QC Process

    Type
    Blog Post
    ...prone to error — both human and biological. Even the most high-fidelity polymerases can make mistakes and ...and highly repetitive sequences and/or features. Most of these regions are identified during the QC process...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    ...Primer binding sites with 40–60% G/C content are most likely to be successful, although sequences with...which helps users select pegRNA designs that are most likely to drive efficient editing. This is not the...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...some engineered terminators come close (>95%). For most purposes, however, any common terminator will suffice...Addgene (1). Prokaryotic polyadenylation Although mostly thought of as a eukaryotic-specific process, prokaryotes...

  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...the target cell’s genome. Physical titer The two most common assays for physical titer are direct p24 ...choice can also impact transgene expression. The most accurate method of lentiviral titration uses qPCR...

  13. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...cell lines, especially the endocrine cell lines we most commonly work with, require extra serum to survive... of the wells in the 48-well plate will grow up, most will eventually grow within a week. We screen the...

  16. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...DNA polymerase from the thermophilic bacterium, Thermos aquaticus (Taq). Taq polymerase is thus a heat ...presence, size, and relative quantity. End point PCR is most commonly used in molecular cloning, sequencing, ...

  17. Pooled Library Amplifications

    Type
    Blog Post
    ... protocol. Created with BioRender.com.   The most important factor to consider is your desired library...what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere between...

  18. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...degrons, you have options! Below we will review the 4 most common types of degrons and some of the nuances ...degradation. HaloTag is also the degron tag with the most alternative functions – there are antibodies to ...

  19. PhD Applications After COVID

    Type
    Blog Post
    ...process of even applying to one of these programs is almost as daunting as the program itself. Picking a mentor...idea to email around three professors per program. Most applications will recommend that you name two professors...
Showing: 461 - 480 of 772 results