We narrowed to 550 results for: NOL

1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ... While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years, prefers...

2. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

   ...plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP...

3. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...sequencing capabilities and Seqwell’s plexWell technology, we can now perform NGS at Addgene to sequence...

4. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   ...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...

5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ...s41596-020-0304-x Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist...

6. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...right. ddPCR uses a water-oil emulsion droplet technology that fractions the PCR reaction sample into approximately...

7. Hot Plasmids: Summer 2025

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   Blog Post

   ...antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based on an antibody-like protein...

8. Hot Plasmids: Winter 2025

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   Blog Post

   ...biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically...

9. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ... development and improvement of CRISPR-based technologies over the past decade. CRISPR screens are easily...

10. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ...viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991...

11. Plasmids 101: Degron Tags

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    Blog Post

    ...Systematic profiling of conditional degron tag technologies for target validation studies. Nat Commun. 13...

12. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post

    ...one of the most powerful tools available to immunologists, allowing for the rapid analysis of cell populations...

13. Of Myc and Men

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    Blog Post

    ...mid-late 20th century with the advent of hybridoma technology and monoclonal antibodies. However, it was (and...

14. PhD Applications After COVID

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    Blog Post

    ...more difficult. I’ve learned that some of the technologies that helped us navigate a global pandemic can...

15. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    ...dominance over HDR in this species. As CRISPR technology continues to develop, it’s become clear that ...

16. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post

    ...updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing purposes...

17. Genome-wide Screening Using CRISPR

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    Blog Post

    ...you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure...

18. Plasmids 101: Multicistronic Vectors

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    Blog Post

    ...selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub...

19. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ...new tools for plant science based around those technologies in the near future. Science on Twitter Addgene...

20. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization...