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We narrowed to 941 results for: OMP;

Showing: 461 - 480 of 941 results

  1. Video Library

    Type
    Protocol
    ...positions he has held in his career, his favorite company tradition, and Addgene's role in the scientific...

  2. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...add another layer of complexity to imaging, as they allow one to monitor compartment/protein dynamics or... may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that GFP can ...Drosophila, and mammalian cells. Photoconverted GFP is compatible with other FPs since its emission wavelength ...

  3. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...expansion, scientists will be able to request an AAV composed of an AAV plasmid in the Addgene repository and...by Addgene for legal approvals and technical compatibility with our production process. This process will...the service to be fulfilled and QC assays to be completed and reviewed. The timeline therefore will be as...follows:  Request review: 1–2 days Prep and QC completed: 6–8 weeks Average shipping time: 1–2 days (USA...

  4. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...recombination by KD, B2 and B3 resulted in complete or near-complete excision of the stop sequence. In contrast...toxicity, but with the caveat of partial rather than complete recombination. If you’re looking to use site-specific...this system. Gerald Rubin’s lab sought to make complex genome modifications in Drosophila using multiple...

  5. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ... the clinic and cures. There is no doubt that companies are swarming to see what can be done with this...the insulin signaling cascades in only 4 months compared to a year or greater with older technologies. ... in the room. He offered the great analogy of comparing the CRISPR/Cas9 system to Norton Antivirus. Antivirus...the code of viruses to give information to the computer to fight the known viruses. Bacteria have figured...

  6. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ... to complete a six month work experience called a co-op to receive a degree. Northeastern compiles a list...Thankfully, my managers set aside time for me to complete an online social media certification on social...petting dogs, and winning the office Just Dance competition.    Special thanks to my manager, Sr. Marketing...

  7. An Addgene Summer Internship

    Type
    Blog Post
    ... had to get used to the workspaces, as well as complete the lab safety and cybersecurity training before...project is a major project given to each intern to complete and present on.    Figure 1: Addgene's...size should be a max of 500 bp (preferably). I compiled a list of three options from the Addgene catalog...Toj-Villagran is a rising senior in high school who just completed their summer internship at Addgene. They plan ...

  8. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction...Sparse Predictive Activity through Recombinase Competition (SPARC), a genetic tool that allows for the labeling...PhiC31 irreversibly recombines one of the two competing attP sites to drive expression of either a stop...is placed downstream of the rest of the SPARC components and expression is controlled by the upstream ...

  9. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...some extra guidance to understand the specific components that make up a plasmid and why each is important...Drives transcription of the target gene. Vital component for expression vectors: determines which cell ...clone into. Regardless, once the cloning steps are complete, the vector containing the newly inserted gene...used in research or for therapeutics Addgene has compiled various educational resources to facilitate plasmid...

  10. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...CRISPR screen data are also available via comprehensive pre-compiled downloads and via a full-featured REST... to stably archived large-scale datasets by computational biologists. Figure 1: The BioGRID Open... from humans and other species. By accurately compiling genome-wide datasets, which are often obscured...

  11. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...question is: how should the gRNAs be oriented in comparison to each other? The gRNAs must target different...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation....preferences using HEK293 cells. They started by comparing D10A and H840A total editing efficiency in PAM-in...donor with 40 bp homology arms. The HDR efficiency compared favorably to using WT Cas9 with the left gRNA ...

  12. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...projects spanning from the identification of plant compounds and insecticidal proteins to the development of... reside within living plants and that produce compounds with various antimicrobial activities (1). She... newly identified proteins and enzymes. These compounds will later get tested for their potential agricultural...alternative source of essential omega-3 fatty acids complementing vegetable oil, a somewhat poor natural source...

  13. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...synthetize all compounds needed for growth, or auxotrophy, the inability to synthesize a compound needed to...an essential amino acid), and a plasmid that complements the auxotrophy (i.e., synthesizes the critical...yeast prototrophy kit containing 23 plasmids to complement auxotrophy. Negative selection: Cells that have...

  14. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...and not solely to ensure a profit. Often, these companies believe they possess something that will truly...the public’s perception of head injury risk. In completing a master’s thesis in science communication, I... I had the chance to carry out a comprehensive review of the literature informing the notion of “heading... Department of Science Communication where he completed a master's degree in 2020, his research explores...

  15. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...platform that allows us to provide scientists with complete plasmid sequences. After launching our viral service...present SMRT sequencing only works for self-complementary AAVs and the TA-based ligation and tagmentation...molecules in the DNA extract associate with complementary strands, enough double-stranded species exist...adeno-associated virus particles containing complementary polynucleotide chains. J Virol 9(2): 394-396...

  16. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...volunteered to do the sampling and I happily agreed to accompany him to YNP the following year. Geoff Schiebinger...microfluidic parallelization to sequence this highly complex hot spring sample. The sample was separated into...cells each for sequencing, allowing for lower complexity with higher throughput and rigorous assembly....ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9...

  17. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize...deoxyadenosine (A) at the 3' ends of PCR products. The complimentary “T” in the pair comes from a topoisomerase I-linearized...overnight. 4. Transform TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol ...transforming your reaction into chemically or electro-competent E. coli - excess salt causes arcing during electroporation...

  18. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...great approach to fully understand and acquire competitive skill sets in a myriad of professions is to engage...Mentorship Leadership Science writing competitions (e.g. Access to Understanding)   Writing...Communication Networking Publicity Business competitions   Leadership Management Marketing ...opportunity to tailor their career paths for competitive positions both within and outside academia. Use...

  19. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...intracellular labeling because the intracellular compartment may contain cross-reacting antigens not present...difference in the efficacy of your antibody. When compared, 4% PFA and 0.1% saponin; 4% PFA and 0.1% Triton...intensity in the KO population (pink histogram) compared to the WT cells (green histogram). The unfilled..., like RNAseq or proteomics, it is possible to compare antibody labeling of different cell types within...expression of the target. In this case, you could compare the antibody’s labeling of the cell lines to the...

  20. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ... bacteria is due to differences in cell wall composition. Cell wall structure Gram positive bacteria, ...is added, it interacts with the CV+, creating a complex within the peptidoglycan layer, staining the cell...the peptidoglycan layer, trapping the large dye complex created by the interaction of CV+ and I- in the...type of bacteria it is being used in, and how components of the plasmid should change based on bacteria...
Showing: 461 - 480 of 941 results