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We narrowed to 1,006 results for: RING

Showing: 461 - 480 of 1006 results

  1. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...stage you could infer that the gene which GFP was monitoring was being expressed in the same pattern. These...result but will still allow for expression pattern monitoring. Enhancer traps Like promoter traps, enhancer...unambiguously identify the gene that they are monitoring as they are directly integrated into it. Enhancer...e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007...

  2. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...can remain present until cleared by the body. Administering the drug once can result in several hours of... Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation to...injection. The main challenge in using ligands is ensuring the agonist is able to cross the blood brain barrier...Bryan Roth lab for feedback on this article and sharing their expertise with us. References Urban DJ, Roth...

  3. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...hydrogen bonds, destabilizing the molecule and favoring the separation of the two helixes. Low pH instead...chromosome. One of the main causes of degradation during plasmid DNA extraction and purification from E....though in their capacity to maintain DNA stability during storage over prolonged periods of time, especially...especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should consider a couple...

  4. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...grow. Auxotrophy and prototrophy come into play during cloning if you’ve designed your experiment using...sucrose. Plasmid cloning: Creativity with naturally occurring genes  At this point in the blog post, you may...negative selection strategies adopt naturally occurring genes for cloning purposes (antibiotic resistance... 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection Resources...

  5. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to stabilize...stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps capture...reducing the read depth necessary when sequencing and lowering the amount of tissue needed per experiment. In...transcriptional regulatory elements is controlled during development using the Drosophila wing as a model...

  6. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ... category already has five blue flame plasmids covering three different epigenetic effectors. The diversity...Bacteria: CRISPR Methods for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing...CRISPR History and Development for Genome Engineering  ...  Mary Gearing...

  7. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...speaker biographies.” During the week of our interview, Hans was also busy preparing materials for an upcoming...took that gamble, and it’s paid off. Work is never boring. “I just keep learning on the job. It keeps it ...mechanism that also looks nice is a great way to bring people in.” Are you interested in careers outside...

  8. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Vandenberghe. We’re excited to grow the team and bring on scientists with a diverse set of experiences....scientist that helps with different aspects of manufacturing and quality, especially with regard to compliance...journey in biochemistry made the foray into genetics during graduate school at MIT. “I read some of the seminal...He’s also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...

  9. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...researcher and ones that happen naturally.  Sample Denaturing  First, let’s think about induced conformation...s configuration in your assay. Did you have a denaturing step? Do you want to capture the protein bound...conditions? It can help to visualize an abstract protein during your sample prep process, imagining how it starts...antibodies that have been validated solely for denaturing assays. Many antibodies will note if they’re ...

  10. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...molecular definition, and potential for further engineering as compared to polyclonal or monoclonal antibodies... in this blog post. Hello, NABOR! Addgene will bring the same open-access benefits to antibodies that...collection. I’m thrilled to be working with Addgene to bring this resource to life,” says Dr. Trimmer. But in...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim Executive...

  11. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... each day brings in new and surprisingly diverse manuscripts. I work with people, nurturing networks, ...regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests, though,...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...

  12. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...academic positions. More recently, I have been hearing exit survey data from postdoctoral programs in ... (every 4-6 years is common), but this can also bring new opportunities—projects end and layoffs occur...change entire culture) Specific requirements for hiring more common Likelier to hire on general talent... paths is an important first step. Developing mentoring relationships, especially with peer mentors, to...

  13. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by ...ordered biochemical events” such as those that occur during development. Read this post to learn more about...research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica Polka,...consider if you’re getting started with them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening...

  14. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C. elegans were released, signaling...revolution!   References Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette....strategies for CRISPR/Cas9 editing in C. elegans: Engineering the Caenorhabditis elegans genome using Cas9-... Mary Gearing...

  15. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Griesbeck is motivated by a particular interest in monitoring the activity and biochemistry of living neurons...allowed the researchers to see tonic action potential firing in neurons and high resolution functional tracking...while in principle adapting a FRET sensor for monitoring calcium versus zinc should require the simple...cells at much lower concentrations than calcium, requiring greater sensitivity. He and his colleagues came...

  16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...innovations have improved the odds of mismatch repair favoring the edited strand. The first iteration of prime...introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch repair Even in PE3 and PE3b, prime editing...of the unedited strand Favor the edited strand during mismatch repair   ✓   ✓ ~ ~ ~ ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,...recombinases and prime editing. Nature Biomedical Engineering. https://doi.org/10.1038/s41551-024-01227-1 Yan...

  17. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary feature... traits. The “biotech yeast” You’re probably wondering if the “biotech yeast” is S. pombe or S. cerevisiae...small, membrane-bound organelles essential for sequestering certain enzymes and performing specific oxidative...

  18. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ... review, and more lab research could be used to bring scientists and the public together to build consensus... it is followed. He’s PCSK9 paper, though not featuring a clinical pregnancy, was rejected by at least...Download the CRISPR 101 eBook Read our genome engineering blog posts Resources on Addgene.org Read our...CRISPR plasmids by function Check out our genome engineering guide ...

  19. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...limitations: they were not optimal for experiments requiring high signal to noise ratios, imaging of high frequency...Create yellow (v82) and lime green (v857) variants differing in kinetics, affinity, and ∆F/F0. Introduce the...and localization through different C-terminal anchoring domains The original iGluSnFR in the pMinDisplay...researchers introduce iGluSnFR3 in two additional anchoring domains – GPI and SGZ. Fig: 4 Sequence...

  20. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...organization, mentoring group, or department. Even if it doesn’t work out, it’s flattering to ask.  Show...Relationships need nurturing. Perhaps you’ve met someone interesting at an event. Or, perhaps you have...There is a person on LinkedIn I once helped by answering one simple question so I accepted the connection...
Showing: 461 - 480 of 1006 results