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  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...restriction enzyme digest and compare it to some reliable controls. 2. Viral stocks may be sensitive to ...reducing the culture volume into which the virus is released, a more concentrated virus solution can be obtained...

  3. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...research goals in focus. Developing these skills relies upon biologists and computer scientists learning...neuron knockouts so that we can perturb factors related to worm motion. Working on the Cat Microbiome Project...

  4. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...are much better at remembering these things on a relaxed phone call than when first confronted on a stage... are for presentations, not discussions. I will rarely work with a panel if slides are allowed. Don't ...

  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...Fusing EGFP to the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could...Chemical amplification of magnetic field effects relevant to avian magnetoreception. Nature Chemistry, 8...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...serotype, as some rAAV serotypes are abundantly released into the culture media, while others remain cell-associated...Wilson, J. M. (2010). Efficient serotype-dependent release of functional vector into the culture medium during...

  8. Pooled Library Amplifications

    Type
    Blog Post
    ...individually count 500,000 colonies, you’ll likely rely on serial dilutions to estimate transformation efficiency... anywhere from 10 ng to 1 μg of DNA. It often correlates with the size of the library, but generally needs...

  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...heartbeat – a muscle contraction – is directly related to the electrical impulse of the cardiac cells....and this voltage is particularly physiologically relevant in neurons, myocytes, and endocrine cells. It ...

  10. Of Myc and Men

    Type
    Blog Post
    ...recombinantly append their proteins of interest with unrelated peptides that already had high quality antibodies...within that region. You’ll notice that “generate a reliable antibody for protein tagging approaches” was not...

  12. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...surrounded by lab members who are all working on related topics, scientific discussion is often part of ...resources on the Addgene blog Check out all careers related blog posts here Find 9 tips for a successful postdoctoral...

  15. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post
    ...is recommended you check your cell lines for HR-related mutations before attempting knock-ins by HDR. ...Designing donors for HDR The basis of the HDR pathway relies on repair from a template molecule, which is usually...

  16. An Introduction to Adenovirus

    Type
    Blog Post
    ...AdVs are ubiquitous (Schmitz et al., 1983), yet rarely cause severe disease in humans (and no vaccines...stages of infection for efficient cell lysis and release of adenovirus from infected cells. J Virol, 70(...

  17. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...polymerase chain reaction Browse our blog posts related to viral vector protocols and tips Read all of ... by qPCR Browse our collection of viral vector related protocols Learn more about the Addgene viral service...

  18. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... One key difference is the plasmid DNA must be released from the bacteria in order to serve as PCR template...reaction. You just need to lyse the bacteria to release the plasmid DNA by either briefly boiling the sample...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...of epitope tags for IHC is the availability of reliable primary antibodies for their detection, particularly...cross-react with other antibodies, or are unavailable entirely. The other advantage is that these tags are small...
Showing: 461 - 480 of 708 results