Addgene

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15 Years of Addgene: The Top 15 Plasmids

Type

Blog Post

...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...
Hot Plasmids - February 2022

Type

Blog Post

...Stasevich and Hiroshi Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, ...
Antibodies 101: Buffers, Storage, and Conjugates

Type

Blog Post

... the buffer, which will be noted on the product sheet. Thankfully, sodium azide can easily be removed ...
Antibodies 101: Isotypes

Type

Blog Post

...Antibodies 101: Introduction to Antibodies for a refresher). There are five antibody isotypes that each have...
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Type

Blog Post

...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines...
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Type

Blog Post

...gene of poorly classified function and a well established cellular signaling gene, thus providing a starting...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

...updated by multiple Addgenies. It was originally published in 2018, in the Viral Vectors 101 eBook, 1st edition...
CasPEDIA: A Functional Classification of Cas Enzymes

Type

Blog Post

... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...
Viral Vectors 101: Viruses as Biological Tools

Type

Blog Post

...specific. Once inside the host cell, the virus unleashes its cargo – its own genome. Fig. 1:...
How Addgene Creates Content

Type

Blog Post

... the example of our Antibody Guide, which was published alongside our new antibody services in the spring...
Developing a cold tolerant E. coli using specialized chaperones

Type

Blog Post

...survive, but thrive, in the face of some of Earth’s harshest environments: corrosive pH ranges, crushing pressures...
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Type

Blog Post

...of endogenous signaling activity in cells. Let’s shed some light on the key components of OptoNBs, how...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Type

Blog Post

...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part...
How to Make Friends and Meet People at a Scientific Conference

Type

Blog Post

...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...
Kiran Musunuru on the Newest TALEN Genome-Editing System

Type

Blog Post

...moment you are designing a TALEN on paper to a finished plasmid. With our protocol, it is feasible to ...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

...using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

...PubMed PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization...

Showing: 461 - 480 of 596 results

Results per page:

15 Years of Addgene: The Top 15 Plasmids

Hot Plasmids - February 2022

Antibodies 101: Buffers, Storage, and Conjugates

Antibodies 101: Isotypes

Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Viral Vectors 101: Gamma-Retroviral Packaging Systems

CasPEDIA: A Functional Classification of Cas Enzymes

GCE4All: Making Genetic Code Expansion Accessible

Viral Vectors 101: Viruses as Biological Tools

How Addgene Creates Content

Developing a cold tolerant E. coli using specialized chaperones

Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

How to Make Friends and Meet People at a Scientific Conference

Kiran Musunuru on the Newest TALEN Genome-Editing System

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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