Addgene

We narrowed to 505 results for: Trac

Showing: 461 - 480 of 505 results

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Hot Plasmids: Fall 2024

Type

Blog Post

...with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker...
CRISPR-based Genome Editing Systems For Editing in E. coli

Type

Blog Post

... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

...examples I’m personally interested in include: intracranial self-stimulation (ICSS) and place preference...
Plasmids 101: Control Plasmids

Type

Blog Post

..., we will take a step beyond discussion in the abstract and follow an example of a plasmid-based experiment...
Deep Mutational Scanning with One Pot Saturation Mutagenesis

Type

Blog Post

...labeled ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the ...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

...The source of those conflicts can frequently be traced back to one of a few trouble spots: Communication...
Antibodies 101: Introduction to Gating in Flow Cytometry

Type

Blog Post

... promise! FSC and SSC: scattering To digitally extract your cells based on their size, the cytometer provides...
CRISPR 101: Homology Directed Repair

Type

Blog Post

...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...
Tips for Getting a Faculty Position

Type

Blog Post

...focus on you and your ideas. Slides will be distracting. What is the big question? This should be...
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

Type

Blog Post

...expression of the transgene. This makes IDLVs an attractive choice for certain types of clinical or basic...
10 Steps to a Perfect Science Talk

Type

Blog Post

...else have a good answer for this?” This doesn’t detract from the speaker, but actually makes for a more...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

...efficiency of the dephosphorylation reaction can be tracked through the comparison of phospho:non-phospho species...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...problem of in vivo CRISPR delivery become more tractable in the next few years, as it would open up many...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

... cloning) and SLiCE (Seamless Ligation Cloning Extract) to name two more. Likewise, there are several ...
Thoughts on the Future of Research 2015 Boston Symposium

Type

Blog Post

...14.3% of PhDs in the life sciences end up in tenure track position 5 years after receiving their PhDs, still...
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Type

Blog Post

... characterised antibodies against all human extracellular proteins with community-based validation. They...
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

... also be a sign that the lab head has trouble attracting scientists. To get a better idea of why the lab...
Recombinase-based State Machines Enable Order-dependent Logic in vivo

Type

Blog Post

...promoters: the BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901...
Generating Mouse Models Using CRISPR/Cas9

Type

Blog Post

...the run, it got “dirty”, with multiple sequence traces overlapping each other! When the count was done...
Building Global Connections with the International Mentorship Program USA-EUROPE

Type

Blog Post

... am not sure that I want to pursue the academic track.” How does an International Mentorship Program work...

Showing: 461 - 480 of 505 results

Results per page:

Hot Plasmids: Fall 2024

CRISPR-based Genome Editing Systems For Editing in E. coli

Important Considerations in Optogenetics Behavioral Experiments

Plasmids 101: Control Plasmids

Deep Mutational Scanning with One Pot Saturation Mutagenesis

Transferable Skills Guide: Conflict Resolution

Antibodies 101: Introduction to Gating in Flow Cytometry

CRISPR 101: Homology Directed Repair

Tips for Getting a Faculty Position

Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

10 Steps to a Perfect Science Talk

Using Phosphoserine to Study Protein Phosphorylation

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Thoughts on the Future of Research 2015 Boston Symposium

Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

Is this the right place for me? 8 tactics for choosing a lab

Recombinase-based State Machines Enable Order-dependent Logic in vivo

Generating Mouse Models Using CRISPR/Cas9

Building Global Connections with the International Mentorship Program USA-EUROPE

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