We narrowed to 508 results for: Trac

1. QC Sequencing Technologies at Addgene

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   Blog Post

   ...the terminating nucleotide and records it in a “trace” file to determine the sequence. Figure 1...

2. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   ...proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ...

3. 5 Reasons to Use Reddit for Science Communication

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   ...Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments Additional Resources See How ...

4. Healthcare Consulting: A Door to the Business of Life Sciences

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   ...roles. Healthcare consulting is a demanding but attractive choice for anyone who wants a fulfilling career...

5. Important Considerations in Optogenetics Behavioral Experiments

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   ...examples I’m personally interested in include: intracranial self-stimulation (ICSS) and place preference...

6. Plasmids 101: Control Plasmids

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   ..., we will take a step beyond discussion in the abstract and follow an example of a plasmid-based experiment...

7. Transferable Skills Guide: Conflict Resolution

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   ...The source of those conflicts can frequently be traced back to one of a few trouble spots:  Communication...

8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ...labeled ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the ...

9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...expression of the transgene. This makes IDLVs an attractive choice for certain types of clinical or basic...

10. Antibodies 101: Introduction to Gating in Flow Cytometry

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    ... promise! FSC and SSC: scattering To digitally extract your cells based on their size, the cytometer provides...

11. Tips for Getting a Faculty Position

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    ...focus on you and your ideas. Slides will be     distracting. What is the big question? This should be...

12. CRISPR 101: Homology Directed Repair

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    ...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...

13. 10 Steps to a Perfect Science Talk

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    ...else have a good answer for this?” This doesn’t detract from the speaker, but actually makes for a more...

14. Using Phosphoserine to Study Protein Phosphorylation

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    ...efficiency of the dephosphorylation reaction can be tracked through the comparison of phospho:non-phospho species...

15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    ...problem of in vivo CRISPR delivery become more tractable in the next few years, as it would open up many...

16. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ... cloning) and SLiCE (Seamless Ligation Cloning Extract) to name two more. Likewise, there are several ...

17. Thoughts on the Future of Research 2015 Boston Symposium

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    ...14.3% of PhDs in the life sciences end up in tenure track position 5 years after receiving their PhDs, still...

18. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ... characterised antibodies against all human extracellular proteins with community-based validation. They...

19. Is this the right place for me? 8 tactics for choosing a lab

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    Blog Post

    ... also be a sign that the lab head has trouble attracting scientists. To get a better idea of why the lab...

20. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...promoters: the BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901...