We narrowed to 762 results for: UTY

...consists of a mutated mCherry and a downstream constitutively active eGFP. To create the inactive mCherry...T2A site and expressed in cells. mCherry is constitutively expressed to identify successfully transfected...

...pathogen or virus detection), therapeutics and sensitive genotyping. The beauty of SHERLOCK systems is that...bottleneck. In the last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a...

...penetration depth as well as high spatial and temporal resolution, making it ideal for visualizing gene expression...also included an analogous set of transient constitutively-expressed mARG plasmids driven by CMV promoters...

...generation of base modifiers available. This system utilizes an RNA template containing the desired edit adjoined...like with base editing, alternative Cas9s can be utilized allowing for PAM flexibility. Fig. 2: Prime...

...accurate than short-read sequencing. At Addgene, we routinely use Nanopore technology to sequence our packaged...written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly...

... developed, though, researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE would allow...recognize T and/or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE...

...invaluable to me as a bench scientist working at an institution where training opportunities weren’t always available...transfer, which occurs when bacteria of different evolutionary lineages share genes that can improve chances...

...opened up vast possibilities for the clinical and therapeutic applications of iPSCs. The generation of human...the production of iPSCs: Blood is easily and routinely drawn from patients in contrast to the process...

.... An internal control may be a plasmid that constitutively expresses a reporter protein (e.g., GFP or ...many variables. However daunting designing and executing these steps may be, proper controls are a basic...

... Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes and...wavelength, to the concentration of fluorophore in solution) are two additional parameters that can affect...

...supported/readable. Is the author or their host institution expected to provide data in formats exported ...resources can offload the burden of data storage and distribution. I would argue that the journals themselves ...

...graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist can...What happens next?”  Describe how you pushed to a solution or overcame obstacles. Take time to consider the...

...demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate HIV-1 transcription...next few years, as it would open up many new therapeutic possibilities for some of the world’s most common...

... in the middle of your insert. Gateway cloning utilizes λ integrase to catalyze directional cloning of...Golden Gate cloning methods. Whether it is for routine cloning, assembly of multiple fragments, or synthetic...

...biotinylating your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining ...

...occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova et al., 2018). For...K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters...

...interview and sell a vision for your contribution to a Department's/Institution's success is the task. Do your...

... applications like long-term imaging or super-resolution techniques. The labs of Pingyong Xu and Zhifei...specific guides. Specifically, the design process utilizes Rule Set 3, a prediction model developed by the...

... genes with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved...the backbone, shRNAs are most often expressed constitutively, either in lentiviral or retroviral backbones...

... is a nearly ubiquitous tool, but proceed with caution! Loading controls only address one type of error...by sample concentration as well as by antibody dilution — both factors we’ve covered in our previous blog...