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We narrowed to 770 results for: VARS

Showing: 461 - 480 of 770 results

  2. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...after the conjugation reaction is done, through a variety of methods or commercial kits.  However, antibodies...create a small, usable panel.  First, fluorophores vary in brightness. You don’t want a super-bright fluorophore...

  3. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... versus other types of antibodies depends on a variety of factors. Understanding the advantages and disadvantages...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080...

  6. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...animal derived product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies...reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti Monster, and the Chicken IgY domain...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

  8. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...   Ask the learners themselves for feedback in a variety of ways, like having them restate or summarize ...you intended (it happens to us all!) and offer a variety of options to make your content more accessible...

  9. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...primary antibody without interfering with the hypervariable region, where an antibody binds to its target...signal amplifiers, the flexibility of their use varies across applications. Western blots, for instance...

  10. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...researchers feel they lack formal training in a variety of transferable skills. At Addgene, we've set out...to apply the scientific method to problems in a variety of contexts.  How do you develop problem solving...

  11. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

  12. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...developed a modular suite of plasmids (pGT) with various replicative origins that can be used for broad ... host-range of the plasmids and suitability of various antibiotic resistance markers, promoters, and inducible...

  15. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...overcome this, lentivirus can be concentrated by a variety of methods such as ultracentrifugation, centrifugation...Finally, choice of concentration method depends of a variety of factors such as resources available, cost and...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    ...for identifying, categorizing, and resolving a variety of QC issues. This process ensures that any potential...making the same decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited...

  18. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...that use CRISPR/Cas9 to endogenously tag a wide variety of genes with GFP. These plasmids are available...gene of interest. Researchers have developed a variety of endogenous gene tagging systems. Addgene’s CRISPR-based...

  19. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ... either 3-base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see... to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly, which used only ...

  20. Hot Plasmids - October 2020

    Type
    Blog Post
    ...were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells grown in high concentrations... Various Addgenies...
Showing: 461 - 480 of 770 results