We narrowed to 573 results for: Vit
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TypeBlog Post...work, although it’s less effective in vivo than in vitro. On the other hand, if you’re interested in expressing...
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Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...gel, or (iii) attached to a substrate. These in vitro methods allow for a well-controlled environment,... -
Visualizing Protein Turnover In Situ
TypeBlog Post...synthesized proteins in situ. I was privileged to be invited to learn from them at the MPI in Frankfurt am Main... -
Antibodies 101: Conjugation
TypeBlog Post...each of these enzymes allow you to detect enzyme activity (and therefore your antibody) either through a... -
To Each HIS Own
TypeBlog Post...variety of ways. How do scientists focus on the activity or function of a single protein? Oftentimes, they... -
All in a Twist: dsRNA
TypeBlog Post...time, even before we knew about all the diverse in vitro production mechanisms. siRNA, also known as RNAi... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...chemogenetics are powerful tools to modulate the activity of neurons and other brain cells. Since the opsins... -
CRISPRainbow and Genome Visualization
TypeBlog Post...When Using Fluorescent Proteins Control Protein Activity with GFP Resources on Asddgene.org Find Fluorescent... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...Impact Are Evoloving Check Out Extracurricular Activities for a Strong Science Career Path Additional ... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...the amount of transcriptional and translational activity a cell has and control how much it taxes the resources... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...underlying causes of this unreliability: cross-reactivity (binds to non-target proteins), variability (... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...quantitative readout of the interaction in vivo or in vitro, and can be used for measuring protein expression... -
Plasmids 101: Modular Cloning
TypeBlog Post...functions. Most individual parts have characterized activities with predictable behaviors. While a gene is the... -
Viral Vectors 101: Virus Safety
TypeBlog Post...how to keep the replication of virus and its infectivity in check. Engineering controls, safety systems... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...but in contrast to lentiviral vectors, their infectivity is limited to dividing cells. Find tips on using... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...Trapnell. "Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy." Journal... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...subsequently destroyed by Argonaute-catalyzed nuclease activity or their translation disrupted through physical... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post... disease. To do so, their T-cells were grown in vitro, their mutations corrected, and the T-cells were... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...given viral stock will give you a sense of the infectivity of your viral stock, save you time, and allow...