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We narrowed to 971 results for: lars

Showing: 461 - 480 of 971 results

  1. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... are just 2 examples of organizations that hold large, annual conferences. Gordon conferences offer more...young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face... Career Expo and the ACS career fair, which are larger meetings offering resources for students seeking...getting a phone interview! If you don’t have a particular employer in mind, you may consider starting with...pages. Make yourself visible on LinkedIn and other popular job site webpages. Importantly, find a good mentor...

  2. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work well with a given...1990s, Gateway cloning technology has become very popular as a rapid and highly efficient way to move DNA...has ready-made entry clones available for many popular genes, including Hs.KRAS4a. Use our website to ...Addgene’s ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables...Diagnostic Restriction Digest Video Check Out Our Molecular Biology Reference Pages  ...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...often used to study the biology surrounding a particular gene (although it has been quite successfully...mutation and need to focus your attention to that particular region. No matter where you are looking, you ...the individual gRNAs in their PX330 plasmids (or similar plasmids for gRNA expression), one gRNA at a time...Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.... 3. Mashiko, Daisuke, et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas ...

  4. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...research. Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...test chamber is used in which a nose-poke at a particular hole (the active hole) induces laser stimulation...without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal activation and is a sufficient... happens. You never know how stimulation of a particular brain region may affect the animal; stimulation...

  5. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...landmark discovery in the 1990s and quickly rose to popularity. Compared to CRISPR, RNAi methods are often quicker...blog, we’ll be focusing on shRNA, one of the most popular choices for gene silencing. RNA interference RNAi...degradation of RNA-induced silencing complex. Molecular Cell, 82(1), 30–43. https://doi.org/10.1016/j.... and assessment of gene knockdown. Methods in Molecular Biology, 139–156. https://doi.org/10.1007/978-...on addgene.org Mammalian shRNA Tools for RNAi Molecular Biology Reference ...

  6. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins or selection...arms between 500-1000 bps. HDR efficiency with circular plasmid templates is generally low; to increase...cerevisiae. Paques F and Haber JE. Microbiology and Molecular Biology Reviews (1999). PubMed. The Road Less ...

  7. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...commonly cited that approximately one-third of cellular proteins are modified through phosphorylation ...generation of pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate bacterial...using the same expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation...Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of cell signaling...Tomida, J., et al. "Detection of phosphorylation on large proteins by Western blotting using Phos-tag containing...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Lichtman, both of Harvard’s Department of Molecular and Cellular Biology, harnessed these attributes of ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...as connections between motor neurons and the neuromuscular junction. In zebrafish, the method has become...neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods...

  9. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...hard work and talent for many years (at a very low salary I might add). You have a right and responsibility...are always traveling, or too busy to interact regularly with the newer lab members. You must get an idea... the lab head? Are meetings with the lab head regularly scheduled or only ad hoc? Does the mentor keep... and learn. Graduate students and postdocs in particular must care about this. The training you get now...at your data and suggest follow up experiments regularly? Can you work on some experiments of your own ...

  10. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...therefore determines the timing of gene expression and largely defines the amount of recombinant protein that ...cumate-inducible gene expression system, which functions in a similar manner to the Tet system. The transactivator configuration...interrogate the activity of uncharacterized promoters. Similarly, placing UAS upstream of a transgene permits directed... binary systems above if they all behave very similarly?” The simple answer is that sometimes it is necessary...systems. Recent work has even used all 3 in a single modular vector for ease of use! Conclusion This post contains...

  11. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...plasmid molar ratio you would like to use for your AAV packaging transfection. In my lab we regularly use ...requires transfecting three plasmids at specific molar ratios. To get these ratios right, you need to do... Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed PMID: 18265393. ...

  12. Editor's Choice, July 2016

    Type
    Blog Post
    ...Choice" series. Each month, I'll summarize the most popular post of the month and point out one or more additional... ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it much easier...surrounding anything to do with CRISPR, our most popular post in July (with over 700 views) was an update...showing that Cpf1 has lower off-target editing and similar on-target editing when compared to the most commonly...

  13. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...Bengyella’s work has focused on elucidating the molecular mechanisms behind interactions between crops like...around the world. Kwabena O. Duedu Dr. Duedu’s molecular biology work has focused on using various cloning...plasmids he acquires through Addgene to produce molecular biological tools like Taq polymerase, T7 polymerase...heavy focus on teaching. He hopes to teach his molecular biology students about the functions of these ...

  14. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...indicates that the amount of leaky expression can largely be attributed to the level of spontaneous recombination...) promoter for catecholamine neurons or glial fibrillary acidic protein (GFAP) for astrocytes it is important...tested in the researcher's system before beginning large scale experiments. This will help to ensure the ...10.1371/journal.pone.0076310 Burger C, Gorbatyuk OS, Velardo MJ, Peden CS, Williams P, Zolotukhin S, Reier PJ...Different Regions of the Central Nervous System. Molecular Therapy 10:302–317. https://doi.org/10.1016/j....

  15. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... you only look at 2 or 3 pairwise comparisons. Similar to family-wise error, is the false discovery rate... to reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this...arranging all calculated p values from smallest to largest and ranking them (1 being the smallest value). ...calculates the cut off for significance by finding the largest p value where its rank divided by the total number...positives/negatives, but they all generally yield a similar result and are appropriate to use if all sample...

  16. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ... Read the molecular biology reference Find plasmids for your research Brush up on molecular biology protocols...as pure as for other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are ...consequence, the preparations for this technique must be larger scale, to allow the extraction of enough plasmid...

  17. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...Toolbox: Free Online Molecular Biology Tools by Beth Kenkel There's a lot of free molecular biology tools out...post. 8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments by Jennifer Tsang Chromoproteins...generate color! They've been adapted for many molecular biology methods such as allelic exchange, FRET...

  18. Let There Be LITE Plasmids

    Type
    Blog Post
    ...limited to particular model organisms or cell types. “This tool is really flexible and modular,” Konermann... vitro by a factor of 10 to 20. A flexible and modular optogenetics tool The researchers also showed just...novel mode of optogenetic control of endogenous cellular processes." The LITE system can now be applied...

  19. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...food on special occasions. However, employees regularly bring in baked goods and we have a few unique ...During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than emergency items) and focus...United States, I look forward to gathering around a large table for a meal with my family. I am thankful for...things, and one is that I get to gather around a large table to eat with my Addgene family every day. Do...

  20. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...transformants. Addgene distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags.... John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification in...at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety of genome... to the collections featured above, many other modular yeast tagging systems have been developed in the...
Showing: 461 - 480 of 971 results