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... While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years, prefers...

...plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP...

...sequencing capabilities and Seqwell’s plexWell technology, we can now perform NGS at Addgene to sequence...

...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...

...s41596-020-0304-x Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist...

...right. ddPCR uses a water-oil emulsion droplet technology that fractions the PCR reaction sample into approximately...

...antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based on an antibody-like protein...

...biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically...

... development and improvement of CRISPR-based technologies over the past decade. CRISPR screens are easily...

...viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991...

...Systematic profiling of conditional degron tag technologies for target validation studies. Nat Commun. 13...

...one of the most powerful tools available to immunologists, allowing for the rapid analysis of cell populations...

...mid-late 20th century with the advent of hybridoma technology and monoclonal antibodies. However, it was (and...

...more difficult. I’ve learned that some of the technologies that helped us navigate a global pandemic can...

...dominance over HDR in this species. As CRISPR technology continues to develop, it’s become clear that ...

...updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing purposes...

...you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure...

...selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub...

...new tools for plant science based around those technologies in the near future. Science on Twitter Addgene...

...2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization...