...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

...over $100 million in annual R&D expenditures to support increased public access to the results of federally-funded...on the global science enterprise lending their support to open scientific research data around the world...online survey of scientists showing overwhelming support for open access to peer-reviewed journal publications...

...sharing ideas for the company, but also talking about sports, movies, and just about anything. As our organization...unique lunchtime traditions that might be fun to incorporate into your own lab. Iron Chef-style competitions...easy as possible. However, I think it’s just as important to take a break from work to share time with the...

...make a reporter construct. Fluorescent proteins (FPs) are also the foundation of multiple important technologies... itself is photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available ...

...work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer...

..., the Addgene board members, vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens...the Addgene family Perhaps the most heartfelt portion of the entire night was when Melina and Ken called... recognized. John and Stephanie Fan have been supporting Addgene from the very beginning and have played...

...had certainly realized the importance of seeking out mentoring as an important aspect to their ongoing success...takes us a decade or more for just the “training” portion of our careers. In that time we may have only 2...

...a scientist in training can adopt to ensure opportunities in the future. Studies show that the majority...ignore this in their cover letters. It is also important to express interest, even excitement, in a position... to highlight different skills and to get the important information in the right place on the page. 5)...

...contribution of students in their labs and the importance of the strong, cross-disciplinary collaboration...Prize. What did Dr. Doudna think was the most important part of their work? First, the discovery of a ... research in the scientific community and the importance of this type of experimentation to the eventual...

...Cepko 1. Work hard. There will be periods when opportunities arise. “Grab it!” 2. Don’t get caught up in ... to deadlines, writing up clear and attractive reports, being good at presentations and of course, networking...from heart and hustle. Each PI emphasizes the importance of passion, hard work, collaboration, and staying...

...surprising, and important, findings being shared. For instance, recently Cellosaurus received a report that HT...cross-references with cell line catalogs and collections. Importantly, Cellosaurus also identifies problematic cell...

...components that make up a plasmid and why each is important. Our “Plasmids 101” series designed to educate...and selectively grown on antibiotic plates.  Importantly, because the bacteria from which plasmids are...expression plasmids, gene knock-down plasmids, reporter plasmids, viral plasmids, and genome engineering...

...worldwide and the presentation made it clear how important it is for us to improve our resources for this...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists know about...

...degree is a means to gain employment, so it’s important to make sure a masters helps you get a job you...from the bench, look for “technical or product support specialist,” “field application scientist,” or ... career ladder is larger and there are more opportunities for promotion. It’s not uncommon for bachelor...

...therapy - avoiding the use of viruses is also important for social and regulatory reasons. 1.) The transposon...puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain...region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows...

...” instead of levels because it emphasizes the importance of these very different roles. At any stage in...doing, managing or leading. All three roles are important to accomplishing any goal or finishing any project... or highlighting criticisms. To be given the opportunity to take on the role of leader, one needs to take...

...attendees an amazing opportunity to share their cutting edge science in increasingly important field.  Interested...through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the day. He was followed...

...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached... as effective for other techniques, but it is important to be aware that the issue is not unique to IHC...

...included in them for downstream control – this is important later! Next, additional components are added -...times more flexible than the brain fibers and importantly distortion of the fibers didn’t affect their ...temperature proximal to the implanted fibers. As important as its capabilities are the practical considerations...

...probes, such as antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs...of antibody-combining sites within the variable portions of heavy and light chains. Proceedings of the ... PMID:4560694. PubMed Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin...