Addgene

We narrowed to 618 results for: proc

Showing: 461 - 480 of 618 results

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Overcoming the Challenges of Lentiviral Production

Type

Blog Post

...While this option is attractive because the same procedure for a single 10 cm2 dish can be easily applied...
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

Type

Blog Post

...lines and use these lines to study how immune processes and biological pathways are altered in PMM2-CDG...
Adeno Associated Virus (AAV) for Cell and Gene Therapy

Type

Blog Post

...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Type

Blog Post

...activation across multiple loci was an arduous process. When using zinc finger proteins or TALE proteins...
Mouse Modeling, Part 2: Breeding and Crossing Mice

Type

Blog Post

...maintain your parental strains throughout this process. In the F1 and later generations, mice that are...
Corralling and Cornering the Goldilocks Antibody

Type

Blog Post

...Schematic of the goldilocks antibody selection process The exposure time of the primary antibody compared...
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Type

Blog Post

...pair. In a sandwich ELISA, the first step of this process is the addition of a detection antibody (typically...
Viral Vectors 101: Calcium Sensors

Type

Blog Post

...to your parameters. Kinetics How fast is the process you would like to monitor? Neurons, for example...
High Complexity Golden Gate Assembly with NEB’s DAD

Type

Blog Post

...genomes, it required a very stringent selection process and benefited from a longer reaction time of 15...
Antibodies 101: Choosing the Right Isotype

Type

Blog Post

...have been cross-adsorbed. The cross-adsorption process filters out off-target antibodies increasing specificity...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

...active site. The RuvC active site in Casɸ can both process crRNA and carry out the crRNA-guided DNA cutting...
Engaging with science and society at pgEd

Type

Blog Post

... and as societal participants in the political process. My role at pgEd I have always been very interested...
Adapting Toehold Switches to Detect Zika Virus

Type

Blog Post

...technology through product development, the regulatory process, and manufacturing so that we can get the diagnostics...
5 Tips for Troubleshooting Viral Transductions

Type

Blog Post

...volume. Always remove packaging cell debris before proceeding to ultracentrifugation to avoid contaminating...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

...overhangs. Exploring the efficiency of these two processes will help us better understand where Cpf1 fits...
Plasmids 101: FLEx Vectors

Type

Blog Post

...SSR of choice is introduced, recombination can proceed either by first utilizing the loxP sites or the...
Tips for Titering Your Lentiviral Preps

Type

Blog Post

...subcloning, expansion, and confirmation. This procedure can take several months but should be considered...
The Materials Science of Optogenetics Experiments

Type

Blog Post

...animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber optic ...
How-to: 5 Steps to a Great Panel Discussion

Type

Blog Post

... Your audience may have a lot of real or self-proclaimed experts on the topic at hand. You can use this...
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Type

Blog Post

... thing into a marketable product. During this process, we had an idea that survived the literature review...

Showing: 461 - 480 of 618 results

Results per page:

Overcoming the Challenges of Lentiviral Production

BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

Adeno Associated Virus (AAV) for Cell and Gene Therapy

CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Mouse Modeling, Part 2: Breeding and Crossing Mice

Corralling and Cornering the Goldilocks Antibody

Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

Viral Vectors 101: Calcium Sensors

High Complexity Golden Gate Assembly with NEB’s DAD

Antibodies 101: Choosing the Right Isotype

Hot Plasmids and Viral Preps - January 2021

Engaging with science and society at pgEd

Adapting Toehold Switches to Detect Zika Virus

5 Tips for Troubleshooting Viral Transductions

Cpf1 Update: Comparison to Cas9 and NgAgo

Plasmids 101: FLEx Vectors

Tips for Titering Your Lentiviral Preps

The Materials Science of Optogenetics Experiments

How-to: 5 Steps to a Great Panel Discussion

Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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