... Martin et al., 2018). The ACE reporter is a bicistronic construct that consists of a mutated mCherry ...eGFP. To create the inactive mCherry, the lab introduced a 43 base-pair insertion that disrupts fluorescence...use across different cell types and systems. To introduce flexibility, the Dow lab created a base editing... optimized cytidine base editor. The lab then introduced an sgRNA targeting the GFPACG to the cells. As...

...founder of Research Space. There are many types of electronic lab notebooks (ELNs), each with its own pros ... virtue of liberating data from paper into an electronic environment and hence making it searchable and... of Research Space, which provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts...on the Addgene blog Find tips on choosing an electronic lab notebook Watch a livestream of the Minisymposium...

...and selection for targets can be done largely in vitro; Easy and reproducible commercial synthesis (those..., Akhtar and Marty, Jean L. “Aptamer based electrochemical sensors for emerging environmental pollutants...systems using immobilized aptamers.” Biosens Bioelectron. 2011 May 15;26(9):3725-36. PubMed PMID: 21419619...FRET in Your Experiments Learn more about GFP Controlling Protein Activity with GFP Resources at Addgene.org...

...social sciences go uncited. Creative titles and controversial topics can garner some attention, though in ...Journal of Number Theory (1,519 views), and Gastroenterology (1,046 views) rounded out the top four average...infections. The video abstracts provide a brief introduction to the research and, after watching the video...links to the videos, keeping the copyright and control over the videos with the researcher and the host...

...protocols therefore differ from library to library. We strongly recommend following the protocols provided by ...a low multiplicity of infection (MOI). MOI is controlled by viral titer, so you’ll need to measure your...Addgene uses a physical titer (via ddPCR) to quality control our viral preps, we recommend determining the functional...transduction protocol you can follow and a troubleshooting blog should you run into any issues.  Keep ...

...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter is...job with a lot of file management that asks for strong organizational skills. You might think, “I’d be...letter The opening paragraph is the place to introduce yourself and your background. It’s a great place...and is important for the job]. Notice that the intros above allow you to highlight what specific skills...

...overexpression vector rather than going through all the troubleshooting required for Cas9-based activation. CRISPRa...Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ (2019...Weissman JS (2014) Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647...2015) A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11...

...reviewer’s concerns, you are making your hypothesis stronger. When you share data at a conference poster session... can frequently be traced back to one of a few trouble spots:  Communication (or lack thereof): Information...Empathizing with people, especially people with whom you strongly disagree, is a critical component of conflict ...tools I’m mentioning here, this may be the most controversial, so I would like to qualify it. If you are attempting...

...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...plasmids encoding aptamer-tagged RNA and later introducing the small molecule into the cell. The fluorescence...metabolites, leading to conformational changes that control the expression of coding RNA. Spinach-based riboswitches...then identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity...

...Level 1 microbe makes it a perfect candidate for introductory teaching and research environments (Idalia &...study human cardiovascular disease, specifically stroke and hypertension (Iannaccone et al., 2009). Rodents...Resource, a breeding facility for X. laevis and X. tropicalis, the two most commonly used species of frog. ... study human disease-relevant genes in such a controlled environment displays how essential the frog is...

...most important ways to spatially and temporally control gene expression, especially in in vivo models, ...Golgi staining, injection of diffusible labels, or electron microscopy of stained sections, were limited due...functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase. When placed under different.... The use of AAV provides spatial and temporal control without the need for germline modification, and...

...as the insertion of a fluorescent tag or the introduction of a specific mutation, one generally relies...one but two rounds of single cell cloning – as a control, one should revert the edit back to the original...technologies offer an alternative to HDR for introducing edits. The same locational constraints are even...the method of producing the guide (synthetic, in vitro transcription, or lentiviral delivery) can affect...

...priorities and needs, but sometimes it just takes introspective thought on what’s worked for you and not worked...reputation? If you are choosing a training lab, I strongly suggest you complete a work rotation in the lab... it could also be a sign that the lab head has trouble attracting scientists. To get a better idea of ...and non-academic career paths. I can’t say this strongly enough - don’t choose a mentor that sees you only...

...formation in the genome and are inefficient at introducing point mutations. In 2016, the researchers at ... Broad Institute envisioned a new strategy to introduce point mutations in the genome. They fused a catalytically...base editors (CBE) use a cytidine deaminase to introduce a C→T change at precise locations in the genome...his super giant albino gorilla fighting off a monstrous wolf and crocodile, that were “affected by CRISPR...

...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

...researcher in the University of Michigan Department of Astronomy. She studies how planets form around young stars...community of science supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is ... an agenda that halts current data collection, destroys existing data, and guts future funding.I marched...march read "You know things are serious when the introverts arrive." I couldn’t watch the denial of facts...

...Correlative light and electron microscopy (CLEM) is an imaging technique that combines electron microscopy (EM...cellular structures (0.5-1% osmium tetroxide). These conditions destroy most PA-FPs, but, in 2015, the Looger...

... Using the FITS Approach Caitlin Faulds, who introduced the very first antibody deposit (the Integrin...flow cytometry expertise in three posts. An Introduction to Gating in Flow Cytometry, Designing Your ...two posts. Flow Compensation and Flow Cytometry Controls Priyamvada Prathima, who wrote a helpful guide...

...initiate our MTA process and perform standard quality control but will not make the plasmids available until ...Continue to Next Step". As part of our quality control process, we will sequence the full plasmid and ...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable...received them and they will undergo our quality control process to verify your plasmids. Our friendly Addgene...

...Podcast breakdown (Time : Topic) 0:00 - 2:00 : Introduction to the podcast from producers Tyler Ford and...and Eddy Page. 2:00 - 3:55 : Alex introduces himself and explains why he decided to do an MD PhD - it's...in generating Cas9 based tools to enable facile control of DNA and RNA.     Special thanks to our sound...