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...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...plasmids encoding aptamer-tagged RNA and later introducing the small molecule into the cell. The fluorescence...metabolites, leading to conformational changes that control the expression of coding RNA. Spinach-based riboswitches...then identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity...

...most important ways to spatially and temporally control gene expression, especially in in vivo models, ...Golgi staining, injection of diffusible labels, or electron microscopy of stained sections, were limited due...functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase. When placed under different.... The use of AAV provides spatial and temporal control without the need for germline modification, and...

...priorities and needs, but sometimes it just takes introspective thought on what’s worked for you and not worked...reputation? If you are choosing a training lab, I strongly suggest you complete a work rotation in the lab... it could also be a sign that the lab head has trouble attracting scientists. To get a better idea of ...and non-academic career paths. I can’t say this strongly enough - don’t choose a mentor that sees you only...

...as the insertion of a fluorescent tag or the introduction of a specific mutation, one generally relies...one but two rounds of single cell cloning – as a control, one should revert the edit back to the original...technologies offer an alternative to HDR for introducing edits. The same locational constraints are even...the method of producing the guide (synthetic, in vitro transcription, or lentiviral delivery) can affect...

...formation in the genome and are inefficient at introducing point mutations. In 2016, the researchers at ... Broad Institute envisioned a new strategy to introduce point mutations in the genome. They fused a catalytically...base editors (CBE) use a cytidine deaminase to introduce a C→T change at precise locations in the genome...his super giant albino gorilla fighting off a monstrous wolf and crocodile, that were “affected by CRISPR...

...Correlative light and electron microscopy (CLEM) is an imaging technique that combines electron microscopy (EM...cellular structures (0.5-1% osmium tetroxide). These conditions destroy most PA-FPs, but, in 2015, the Looger...

...researcher in the University of Michigan Department of Astronomy. She studies how planets form around young stars...community of science supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is ... an agenda that halts current data collection, destroys existing data, and guts future funding.I marched...march read "You know things are serious when the introverts arrive." I couldn’t watch the denial of facts...

...initiate our MTA process and perform standard quality control but will not make the plasmids available until:...Continue to Next Step". As part of our quality control process, we will sequence the full plasmid and ...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable...received them and they will undergo our quality control process to verify your plasmids. Our friendly Addgene...

... Using the FITS Approach Caitlin Faulds, who introduced the very first antibody deposit (the Integrin...flow cytometry expertise in three posts. An Introduction to Gating in Flow Cytometry, Designing Your ...two posts. Flow Compensation and Flow Cytometry Controls Priyamvada Prathima, who wrote a helpful guide...

...Podcast breakdown (Time : Topic) 0:00 - 2:00 : Introduction to the podcast from producers Tyler Ford and...and Eddy Page. 2:00 - 3:55 : Alex introduces himself and explains why he decided to do an MD PhD - it's...in generating Cas9 based tools to enable facile control of DNA and RNA.     Special thanks to our sound...

...ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, but you...occurring earlier but that the photo evidence was destroyed to protect the innocent revelers. Our Halloween...

...interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter ...the importance of sharing information/knowledge Outro Many thanks to our guests, Melina Fan, Kenneth ...nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's Viral Service - Why Virus? Why Now? ...

...AAV9 tdTomato virus in the mPFC.  The AAV9 is so strong that you can even see dendritic spines!  Curious...Images that clearly show viral spread and cellular tropism* (min. 300 dpi, PNG format) At least two replicates... the target region, if the report describes a retrograde serotype An abstract that summarizes your experiment...

...grant to order plasmids she can use as positive controls in PCR-based cervical cancer screens. This third...predominantly HPV 16 and 18.” HPV plasmids as positive controls To start her work, Gladys will improve cervical... containing a variety of HPV genes as positive controls in her PCR screens. She hopes that these screens...

...projects 0:28 - 1:12 : Introduction from Tyler 1:13 - 2:02 : Niroshi and Ben introduce themselves 2:03 - 3...your biggest experimental success? 31:32 - end : Outro In our next episode, Niroshi and Ben discuss how...

...parental cell line, but not in the iso-genic knockout control. This can involve cell surface labeling and/or ... Additionally, it can be a real challenge to troubleshoot RNAi failures. Potential explanations for RNAi... target of interest, you can add an additional control containing treated cells. The expected change in...55133-7 More resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: ...

...the performance of different nucleases. Nicola Patron’s lab compared the efficiency and specificity of...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit...has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus...

...They kicked off their day with breakfast and an introduction to Addgene with our Executive Director, Joanne...in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology Lianna ...our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how much work is...

...for use as a negative control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM...psPAX2. Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past...

... they do so by activating signaling pathways controlling properties like growth, survival, and differentiation...inactivated the pathway. “The idea is that if you introduce them into cells one at a time, you could identify... for pathways that drive resistance to drugs in vitro, and they’ve shown that it works. But, he says that...