Addgene

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Showing: 461 - 480 of 529 results

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Viral Vectors 101: The AAV Retrograde Serotype

Type

Blog Post

...reagents, AAV particles require receptor-mediated entry into the cell. This means that not all neurons and...
Building and Validating Recombinant Antibodies: A Case Study

Type

Blog Post

...functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method was chosen because ...
Antibodies 101: Monoclonal Antibodies

Type

Blog Post

...Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007. https://doi.org/10.1074/jbc.m116.736942...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...Genetics and Molecular Biology, the Dept. of Biochemistry and Biophysics and the Lineberger Comprehensive...
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Type

Blog Post

...is. While communication styles in academia and industry vary drastically, he reassures that one can learn...
Plasmids 101: Yeast Vectors

Type

Blog Post

... multiple copies are required. yes TRP1 L-tryptophan yes - Grow with 5-FAA. S. cerevisiae no no TRP1...
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Type

Blog Post

...potluck dinner. Invite guests from a lab, career or industry of interest – learn about their career paths and...
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Type

Blog Post

...and suggestions, and even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

... Stefan Hell, who won the 2014 Nobel Prize in Chemistry for his contributions to super-resolution light...
Reproducibility for Everyone: Lessons from an Open Science Collaboration

Type

Blog Post

...that it is driven by academics, with support of industry partners, not the other way around. Sometimes,...
Celebrating 15 Years of Scientific Sharing

Type

Blog Post

...request from us but also by high school students and industry scientists as a universal resource for information...
Viral Vectors 101: An Introduction to AAV

Type

Blog Post

...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...
Antibodies 101: Affinity Reagents

Type

Blog Post

...Antigen Binding Format. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.M407888200 Kwon, ...
Developing Lab Management Software for Biology

Type

Blog Post

...If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab staff. ...
Plasmids 101: Cre-lox

Type

Blog Post

...-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives the loxP site directionality...
Working with Nuclear Receptors

Type

Blog Post

...adipogenesis and macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of...
Popular Retroviral Vectors and Their Uses in Scientific Research

Type

Blog Post

...from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral Destination vectors. These...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...
Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Type

Blog Post

...Doug Warner on the right) has taught courses in biochemistry, molecular cell biology and genetics at Boston...
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

Type

Blog Post

...Regulation by Long Noncoding RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146...

Showing: 461 - 480 of 529 results

Results per page:

Viral Vectors 101: The AAV Retrograde Serotype

Building and Validating Recombinant Antibodies: A Case Study

Antibodies 101: Monoclonal Antibodies

CRISPR 101: Non-Homologous End Joining

Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Plasmids 101: Yeast Vectors

Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Reproducibility for Everyone: Lessons from an Open Science Collaboration

Celebrating 15 Years of Scientific Sharing

Viral Vectors 101: An Introduction to AAV

Antibodies 101: Affinity Reagents

Developing Lab Management Software for Biology

Plasmids 101: Cre-lox

Working with Nuclear Receptors

Popular Retroviral Vectors and Their Uses in Scientific Research

Adenoviral Vector Production and Troubleshooting

Pathways Over Time Plasmids Engage Students in Functional Genomics Research

CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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