We narrowed to 954 results for: COMP

...easier to identify by comparing peak positions to the base expression. When compared to the control — which... voltage has been adjusted appropriately and compensation performed. Before you read out your plots, you...to make sure you’ve also adjusted voltage and compensated your colors. Here are some good practice tips...population cluster would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity...place your gate based on the dot plot would be complicated.  Figure 2: (A) a smeary dot plot showing...with the baseline suggesting this sample has a comparable expression to the baseline/negative control. ... populations in the histogram plot in Figure 4 compared to the dot plot in Figure 2. Quantification using...

...protein:DNA complexes with antibodies specific to your protein(s) of interest. Once the complex is captured...protein:DNA complexes and finally (5) isolated the DNA from your antibody:protein:DNA complexes. Your DNA... the first step is to ensure your protein:DNA complexes will remain bound to each other until you are ...reversible bonds. This will keep the protein:DNA complexes together until you want to break them. Crosslinking...digestion can preserve more of the protein:DNA complexes. Once you’ve finished fragmenting the DNA, spin...that?  So while this method is certainly long and complex, you may be thinking, well, these are mostly fairly...techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques used, but from ...

...that the classroom cannot offer. Furthermore, companies across most industries tend to hire their former...Prior to March 2020, internships were a fiercely competitive endeavor. Due to the COVID-19 pandemic, students...college, and graduate students. In order to gain a competitive advantage this cycle, consider the advice below...which is the perfect opportunity to paint a comprehensive picture of your academic interests, potential... impress the judges on your ability to tell a compelling narrative that is positive, and forward-looking...counterintuitive since that approach seems to create competition for your own spot. True! But you create a network...sense approaches that will prepare you for the competitive landscape beyond college. They will also serve...

...career at a biotech company, I always assumed I’d work one or two years at the company and then go to grad... the head of software product management at my company was open to meeting with me and explaining more...software product manager helps ensure that the company or software group is building the software tools...Well, I’ll give you an example. At my previous company, Ginkgo Bioworks, our software development team...backgrounds, most did not. It was my job to translate complex lab operations workflows into a format the developers... to solve is clear, but the solution is more complicated. You’ll need to be creative to be able to work...framework If you can’t find internships at science companies, try to find internships with translatable skills...

...requirement if you work for a private company you must show that your company employs at least 3 full-time people...Within these sectors, you could start your own company, work in a bench research career or a non-bench... to find the perfect job immediately after you complete your Ph.D. or postdoc. Foreign national researchers... need to be aware of the April 1 deadline for companies to submit an application on their behalf.  If ...technology transfer, communications, medical writing, compliance, core facility management, collaboration management...be directed to you specifically. You must also complete the peer review for it to be counted as proof ...your CV and bio sketch whenever you have new accomplishments to add. Obtain copies and translations of documents...

...challenging for sequences with complex secondary structure. Today, multiple companies offer long single-stranded...ribonucleoprotein (RNP) complexes of Cas9 protein and the separated guide RNA components (crRNA and tracrRNA...months. With CRISPR (B), you can inject CRISPR components directly into a zygote and obtain a knockout ...maximum Easi-CRISPR efficiency. Easi-CRISPR is compatible with standard microinjection and electroporation...successfully used guides targeting the same or complementary strands, as well as guides facing towards or... simple method for delivery of genome editing components to the germline. In contrast to standard microinjection...placed to mediate electroporation. Ohtsuka et al. compared traditional microinjection and i-GONAD knock-in...

...engineered ESCs), and chimeric adults mated to produce completely transgenic offspring. Although powerful, this... genome, the CRISPR/Cas system makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies...gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected into either...eggs. Or, to avoid handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant... major advantages of CRISPR mouse editing when compared to more conventional methods. First, one can work... work with almost any strains of mice, as compared with conventional gene targeting, which is limited ...including 129 and C57BL/6, for which we have germline competent ESC lines. Second, the process is much quicker...

...ask your interviewers You’re interviewing the company as much as they are interviewing you, so come prepared...performance evaluations, professional development, and company culture.  It’s also a good idea to prepare 1-2 .... This is a chance to highlight your prior accomplishments and demonstrate your science communication ... Connect your prior work or a new idea to the company’s area of expertise. This has potential to make ...consider bringing your own laptop as a backup, if the company will allow it. Before you start your presentation...interview. Expect to be tired once the interview is complete Industry interviews are long! Mine lasted 4.5 ...read emails from HR carefully because sometimes companies have relaxed dress codes for interviews. You can...

...MiSeq that takes about two days to complete. After the run completes, we begin our assembly process. Again...sufficient quantity for sequencing. This process is completed in a plate format yielding anywhere from two to...Illumina sequencing on our newly donated MiSeq.  For Complete Plasmid Sequencing we perform a 2x251 run on our...annotated Genbank files that are useful for more complicated plasmids, like those that contain synthesized...mutations, truncations, and insertions that could compromise function. When we do find mutations, we check..., NGS for some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids...obtain an NGS result that is useful, but not 100% complete, we will still make this data available as a partial...

...your product’s web page, or in the company’s FAQs? A lot of companies and organizations selling biological...answering help emails and phone calls, I feel compelled to share my years of accumulated wisdom so that...information, but it’s not in the best interest of any company to hide information about their products from you...which case, you should probably stop using that company). Frequently asked question pages on website are...customers (and I expect this is the case at most companies). Always go back to the source. Although many...experience: Make sure you’re contacting the correct company. This might seem like a no-brainer, but it happens...please share the data. Maybe not all, but many bio companies have real scientists answering their technical...