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We narrowed to 1,010 results for: RING;

Showing: 241 - 250 of 1010 results

  1. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ... organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows us ...laborious handling (screening 768 F1 animals). Recovering rare editing events in a sea of unedited animals..., which makes screening extremely rapid. This stringent selection allowed me to optimize a range of editing...al., 2018). They further built on this work, discovering that melting and rapidly cooling dsDNA repair...also found that the majority of knock-ins were occurring 24 hours post-injection, making screening for ...where the 5’ ends of the PAMs are facing outwards, ensuring the PAMs remain on the chromosome after DSB formation...Toolkit and SapTrap-SEC kit for C. elegans genome engineering have also facilitated CRISPR editing in C. elegans...

  2. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the...the fields of molecular biology and bioengineering, as it has facilitated the development of a simple ...relative nuclease efficiencies as well as to more stringent targeting rules that result in a decrease in available... as a means of modulating gene expression (6). Altering the length of gRNAs targeting different sites ...Santa Cruz, where her research is focused on engineering nanoparticles as drug delivery systems. She is...develop several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.   ..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

  3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...and can be used for most of the current genome engineering applications of CRISPR including: generating ...delivery. You’ve decided to use RNPs, now what? Preparing the Cas9-gRNA ribonucleoprotein The first step... the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An advantage of...applications. This technique utilizes Cas9 proteins harboring receptor ligands (C in the figure above), which...stable in human plasma than the standard spCas9 (Harrington et al. 2017). Lastly, as with any scientific ... Addgene.  Andrew's interests include genome engineering and microbiology.     References 1. Chen, Sean...

  4. DNA Purification Without a Kit

    Type
    Blog Post
    ...proteins in solution. The pH is then lowered using a renaturing solution, which causes the proteins and genomic...purification and DNA gel extraction with glass syringe filters Starting material: bacterial cultures or...binding DNA to silica columns, it’s bound to glass syringe filters. Normally DNA does not bind silica or glass... kit takes 1-1.5 hours to complete, while this syringe-based method can be completed in 20 to 30 minutes...culture you’re working with. Overall, the glass syringe filter method is not only cheaper, but also faster...gel slices Product: DNA fragments You might be wondering, “How is milk going to help me extract DNA from... 48 kb with high yields and without degradation during the extraction procedure. Additionally, purified...

  5. Reflections and Looking Ahead

    Type
    Blog Post
    ...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs... to make it easier to find what you need and to bring it in line with current Web Content Accessibility...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al...packaged on demand in five distinct serotypes, offering you unparalleled flexibility. But this is just...characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists, and developers...collaboration formed, and every breakthrough enabled brings us closer to making an impact on humanity's greatest...this incredible journey. Together, we're not just sharing research materials — we're accelerating the future...

  6. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ... Members periodically break, go exploring outside the team, and bring information back. No team has all...contribution short and sweet. One key to this is ensuring that the group always conducts exceptionally well-managed...manager can be efficient without delegating and empowering the team to solve problems together (tactics ...important paths of information flow is the one that brings feedback back to the manager. Team members rarely...often A one-time event doesn't work to get people sharing.   Frequently ask direct questions for specific...at formal communications events or at informal gatherings, like at lunch. Many experts recommend using ...constructive feedback and suggestions. Consider offering rewards (gift card, afternoon off, etc.) Act on...

  7. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...important in a non-research context, especially when considering small to mid-sized companies and organizations...time I’ve had a role in organizing collaborations. During my graduate studies at Karolinska Institutet I ...what was expected, and keeping track of speakers. During your graduate or postdoctoral work there are loads...you develop will lead! Read our blog post about fostering communication in remote collaborations! Stay in...Great progress! You are collaborating with your neighboring lab, you've met interesting people at conferences...twitter and maintain a connection by commenting and sharing. LinkedIn or ResearchGate allow you to send personal...

  8. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ... your foot in the door When I was first considering preparing for and transitioning to a career in healthcare... Healthcare consulting is a fast-paced field, requiring people who are willing to quickly learn, and apply...governments on the most effective and efficient ways of delivering healthcare. Clients typically engage consultants...fields. I got my PhD a couple of years ago in Bioengineering, and one of the things that pleasantly surprised... suite of benefits a given therapeutic approach brings to patients, doctors, and insurance providers, ...aspect of research in biology or medicine you will bring an essential set of tools to a consulting team. ...an increasing recognition of the value graduates bring to these roles. Healthcare consulting is a demanding...

  9. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...Read here to get help if you are having trouble preparing your most excellent resume. 2) Follow job boards...reviewed regardless of personal connections to the hiring group.  Read here for more on timely submission... job description is usually a wish list and the hiring manager knows that no applicant is likely to meet...Your resume must be in the official “system” for hiring managers to take next steps and follow up especially... as you will not likely know the exact person monitoring submissions (usually a Human Resources representative... your resume pdf document. 6) Use your network! During the entire process, use whatever means you can ...can to get your resume into the hands of the hiring manager on paper or as an email send directly from a...

  10. An Addgene Summer Internship

    Type
    Blog Post
    ...C., Addgene’s intern coordinator shortly after hearing her talk about Addgene’s internship opportunity...summer interns presenting their capstone projects during the Summer Intern Poster Presentation.  As ...the Syntaxin3 gene in a pLenti vector backbone. During those five weeks, I worked with my mentors, Katherine...always came with surprises. Originally we thought during the first three weeks that we had made a mistake...not having the right concentration of a reagent during our HiFi assembly but decided to go ahead anyway...restart the procedure we would not have enough time. During the following two weeks, we were able to obtain...on my capstone project report and presentation, during the final week at Addgene. This report shared my...
Showing: 241 - 250 of 1010 results