We narrowed to 872 results for: cel

...Cellular communication and function depend on potential energy. Cellular activities like action potentials...Indicators As previously mentioned, cellular voltage is measured at the cell membrane, and this voltage is ...endocrine cells. It follows that the specific targeting of these voltage indicators needs to be cell type ...electrical events! Voltage indicators are voltmeters for cells! In this blog we will review how these indicators...related to the electrical impulse of the cardiac cells. To study this phenomenon you will likely want to...the voltage change across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by... electrical charges propagated through multiple cells to do things like translate perceived signals to...

...packaging cell line. γ-Retroviruses can transduce target cells, but the transduced target cell does not...using helper-free packaging cell lines This method utilizes a packaging cell line that stably expresses... packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging cell line developed...and rat cells), or an amphotropic envelope, Phoenix-AMPHO (for the infection of mammalian cells.) Using...transduce both dividing and non-dividing cells because once in the host cell cytoplasm, the viral components are...for efficient gene delivery to mammalian cells using 293T cell-based systems. Curr Protoc Immunol. 2001...γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple...

...the caffeine of the cell!)—ATP. There are biosensors for intracellular, extracellular, and even the ADP:... as proxies for a cellular process or directly measure a cellular process. Cellular phenomena such as ... measure real-time cellular reactions to stimuli and as well as changes in cellular dynamics during so-called...type of cells the viral particles infect. This can direct your biosensor to specific tissues or cell (sub...Applications. Frontiers in Cellular Neuroscience. 2019 Oct Vol. 13; 1662-5102. DOI: 10.3389/fncel.2019.00474 Resources...biosensor is the thing that you care about within your cellular or animal system. This ‘thing’ could be a neurotransmitter...blog we will focus on those which can be applied in cell culture and animal models primarily. Fun fact: Almost...

...animation.)  Many types of immune cells, from mast cells to B cells, express Fc receptors that bind to...) antibody-dependent cellular cytotoxicity (ADCC) c) antibody-dependent cellular phagocytosis (ADCP). ...opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive when holes...die.  Antibody dependent cellular cytotoxicity (ADCC) Antibody dependent cellular cytotoxicity (ADCC) is...(NK) cells expressing Fcγ receptors binds to IgG and release cytotoxins that kill the target cell. If ...response, by binding to Fc receptors on other immune cells. (psst! Need a quick review of the different immune...pathogen and physically prevent it from entering a cell, a non-Fc-dependent effector function known as neutralization...

...lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted to ...reporter output was observed in their 293 cell culture system. In cells cotransfected with GFP and the GBPs,...expression was induced in ~76% of GFP+ cells, and 100% of DsRed+ cells also expressed GFP. Using AAV constructs...fluorescent proteins as scaffolds for cell-specific gene manipulation.” Cell 154(4) (2013): 928-939. PubMed ...wanted to express a variety of proteins in single cell types in the mouse, but knew it would take years...could then selectively manipulate only GFP-labeled cells. Once Tang and Cepko found a description of GFP-...heavy chain, and they are small and stable inside cells. Nanobodies binding GFP were designed in 2009. Subsequent...

...resistant cancer cells exist prior to drug treatment. They infected a non-small cell lung cancer cell line containing...same parent cells that represent the preexisting resistant subpopulation 2. Resistant cells among the separate... target cell population at low multiplicity of infection (MOI) such that each individual cell receives...researchers to individually label millions of mammalian cells through lentiviral infection and to monitor their...quantify the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to...ClonTracer library to determine whether or not cancer cell populations resistant to drug treatments arise during...They hypothesized that, if they labeled the parent cell population using the library, propagated it, separated...

... between living cells by TransitID. Cell, 186(15), 3307–3324.e30. doi: 10.1016/j.cell.2023.05.044. PMID... efficient prime editors. Cell, 186(18), 3983–4002.e26. doi: 10.1016/j.cell.2023.07.039. PMID: 37657419...lentivirus and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying...Optogenetic proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching an old...called DIRECTED (Delivery to Intended REcipient Cells Through Envelope Design). This modular approach ...viral surface, expanding the range of targetable cell types. DIRECTED is compatible with fusogens from...process and could even enable the creation of CAR-T cells directly within the body. Overall, DIRECTED can ...

...PV-positive cells out of all BiPVe3-dTom cells. C) Confocal stack of a biocytin-filled cell expressing ...particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected...interactome. Molecular Cell, 84(24), 4929-4944.e8. https://doi.org/10.1016/j.molcel.2024.11.007. Branon,...Scheme for two-photon (2P) imaging and loose-seal, cell-attached recordings in primary visual cortex (V1...contains everything needed to replicate inside a host cell but with space to insert your genetic cargo of choice...promoter drives robust gene expression in mammalian cells. Flexible tagging: Choose untagged or add a versatile...stable: A puromycin resistance gene makes stable cell line creation a breeze. Modular by design: Key elements...

...epithelial cells, liver, CNS (limited penetration), retina, hematopoietic cells, and immune cells. B) AAV11...Sanchez, P. S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing stem cell heterogeneity dictates clonal...the acquisition of leukemic driver mutations. Cell Stem Cell, 32(4), 564–580.e6. https://doi.org/10.1016...therapy and ex vivo cell engineering applications and is especially well-suited for lung cells via airway administration...plant genome editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging...deposited a variety of constructs for visualizing cellular components (nucleus, endosomes, lysosome, golgi...filaments with mGold2. Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s, or ...

...prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells. The... continuous cellular processes (e.g., protein synthesis), while amp only affects the cell wall synthesis...Check icon" %} Spectinomycin Excellent No Just bacteria Not SHuffle cells {% icon icon_set="fontawesome...icon" %} Zeocin Excellent No Bacteria, plants, and eukaryotes Not Tn5-containing cells {% icon icon_set..., penicillin, and is a beta-lactam that inhibits cell wall synthesis. The AmpR (amp resistance) gene produces...synthesis step during cell division. This is nifty because the recovery period after transformation (the dreaded...at 37 degrees) can be shortened since the plated cells will have time to produce beta-lactamase prior to...