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Suggest A Plasmid You'd Like to Find at Addgene

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Blog Post

...suggestaplasmid, the name of the plasmid and a publication where we can find the plasmid. While we cannot...
The 12 Days of CRISPR: 2021

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Blog Post

...On the ninth day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration. ...
It’s CRISPR Week at Addgene!

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Blog Post

...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...
New FLIS Plasmid (April Fools 2022)

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Blog Post

...well, annoy. Fly-related Issues in the Lab A significant drawback of working with fruit flies is their...
We’re Going Viral This Week!

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Blog Post

... information on the basics of viral vectors, applications, and some new resources available from Addgene...
CRISPR 101 eBook...en español

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Blog Post

...traducción, Addgene contrató a una traductora científica profesional, L. Denise Roche, para editarla. ...
New Videos: Addgene Lab Tips

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Blog Post

...your life a little easier. These can range from technical tips as in our episode on Barcoding, to basic ...
Twenty Years of Sharing Science!

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Blog Post

... you know you can search for plasmids from a publication by pasting the paper's title into the Addgene...
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

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Blog Post

...Callaway For researchers in countries with applicable export control exceptions for rabies and VSV-...
Top Requested Plasmid of 2017 - pMD2.G

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Blog Post

...a technology is so useful it forever enhances biological research and discovery. CRISPR is a great example...
Celebrating the 2024 Nobel Prize in Chemistry

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...understanding of how protein folding works and creating biological tools not found in nature. Dr. Baker’s team was...
Addgene’s New Way to Help You Share Science

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...Addgene. Learn how discoveries were made and get practical tips on how to use the plasmids in your own lab...
Streaking for Single Colonies: The Streak Plate Challenge

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...February 13, 2021 Westenberg, a Professor of Biological Sciences at Missouri University of Science and...
PEI Calculator for Planning AAV Packaging Transfections

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Blog Post

...considering using this PEI Calculator. AAV packaging typically requires transfecting three plasmids at specific...
Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

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Blog Post

... plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create...
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

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...streaking to a single colony to DNA extraction and quantification so that you can focus on what you want to do...
2018 #DeckTheLab Contest Winners

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...wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication #sciencedaily #instascience #ladies #research...
Visualizing Your Plasmid Request Data

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Blog Post

...Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes. You can follow him...
AAV Packaged on Request is Here!

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Blog Post

...Once your request is received, we’ll review for technical and legal compliance, and then send you an email...
Addgene’s Top 10 Blog Posts from 2014

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Blog Post

...Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making...
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

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...Hiroshima University. “However, there had been no systematically established way of making an all-in-one vector...
How Do I Place an Addgene Order Using a PO Number?

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Blog Post

...plasmids, store your plasmids or viral items, technical support, and more. ...
2022 and 2023: Reflections and Looking Forward

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Blog Post

...curated information about AAV usage in research applications. Scientists around the world can upload AAV ...
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

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Blog Post

...of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay...
Your Top Requested Plasmid in 2016!

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Blog Post

...as links to the Zhang Lab's Nature Protocols Publication describing them here. References 1.Ran, F. Ann...
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

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...KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase reporters. A KTR is...
Stepping into a Virtual Internship: My Experience at Addgene

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Blog Post

...right path. The frequent Zoom meetings helped me communicate my ideas. I was able to express my visions for...
Our Most Popular Blog Posts from 2019

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Blog Post

...Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...
Six Spooky Science Stories and Halloween at Addgene

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...approach." Proceedings of the Royal Society B: Biological Sciences 272.1577 (2005): 2117-2126. PubMed PMID...
Inside Addgene's 10 Year Anniversary Gala

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...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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... a Cas9 nickase and two gRNAs. For nickase applications, a common question is: how should the gRNAs ...
New CRISPR Web Resources and #12DaysofCRISPR Recap

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...providing an avenue for temporary/reversible CRISPR modification. In addition to these subpages, our updated ...
Bringing Sustainable Practices to the Lab: Recycling

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...easier to ship back. Be that person! If you use serological pipettes from Corning®, Falcon®, or Axygen®, ...
Plasmids 101: Positive and Negative Selection for Plasmid Cloning

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...complements the auxotrophy (i.e., synthesizes the critical amino acid). Many common E. coli strains used ...
Some Like it Hot: Thermostable GeoCas9

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...could also be used with in vitro molecular bio applications that require high temperature reaction conditions...
Management for Scientists: Giving Feedback

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Blog Post

... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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Blog Post

...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...
Safe Port in a Storm...How Addgene is Weathering the Pandemic

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...requests for scientists doing almost every type of biomedical science research. Our work accelerates and enhances...
New and Upcoming Viral Vectors - December 2019

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Blog Post

...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...
Components of CRISPR/Cas9

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Blog Post

... to modify the desired genomic locus. Some historical notes CRISPR arrays in bacterial genomes consist...

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