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We narrowed to 960 results for: App

Showing: 481 - 500 of 960 results

  1. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...Science magazine. That one I just could not resist applying for. Not that I knew anything about being an editor...constellation of interests and skills than what fits for applied research. I focus in general terms on a couple ...Review and Scientific Publication, where diverse approaches to peer review were discussed. Science communication...

  2. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...chatting makes it pretty easy to make 30 minute appointments with people all over the world. Think about ...are many different kinds of grants that you can apply for. If you are in a lab, your PI is likely working...working on a number of them. Find one to apply to or offer to help with lab grant writing. You may not get...

  3. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...on the marketing or business background of the applicant. However, although this position did look for ...experience or a marketing interest that would be able to apply themselves immediately to the position. In case ...understand enough to relate it to the position I was applying for. After all I was only going to be interviewed...

  4. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...your meetings productive: Discuss logistics if appropriate:  How often will you meet? How does the Mentor...the process of working on them is where growth happens. Mentors bring more than their knowledge and teaching... get a bunch of text messages asking me, “What happened? Where are you?” My gym posse holds me accountable...

  5. Protocol - Bacterial Transformation

    Type
    Protocol
    ... thaw on ice (approximately 20-30 mins). Remove agar plates (containing the appropriate antibiotic ) from...spreading device Reagents LB agar plate (with appropriate antibiotic) LB or SOC media Competent cells DNA...Pro-Tip Transformation efficiencies will be approximately 10-fold lower for ligation of inserts to vectors...transformation onto a 10 cm LB agar plate containing the appropriate antibiotic. Pro-Tips We recommend that you plate... take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce membrane permeability... to have access to an electroporator and the appropriate cuvettes. Follow the manufacturer's instructions...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...your convenience. See “warranty information” in appendix. Table of Contents A. pLKO.1-TRC Cloning Vector...References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence of pLKO.1 TRC-Cloning Vector I.2 ...GenHunter: #Q401 FuGENE® 6 Transfection Reagent Roche Applied Biosciences: #11814443001 OPTI-MEM® serum-free ...protamine sulfate, and puromycin are located in the “Appendix”. F.2. Determining the Optimal Puromycin Concentration...overnight. Day 2: b. The target cells should be approximately 80-90% confluent. c. Dilute puromycin in the...increments. d. Label plates from 1-10 and add appropriate puromycin-containing media to cells. Days 3+:...overnight. Day 2: b. Target cells should be approximately 70% confluent. Change to fresh culture media...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...used Parkinson’s experimental model. In fact, approximately 25% of yeast genes have a human counterpart,...2017). Beyond human relevance, yeast have many applications in food science and biotech due to its fermentation... ancient yeast strains and metazoan cells.   Applications for K. phaffii There’s a number of different...

  8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique isoforms transcribed...single TF was delivered to each cell, future applications could deliver multiple TFs to each cell, allowing...and/or TF dysregulation. A targeted TF screening approach can not only lead to a better understanding of...

  9. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...bioanthropology. Studying human health science made me happy, but it wasn’t long before I started becoming disillusioned...drives better after a few drinks. A family member approached me for advice on her baby’s health but adamantly...abstract where the real medical conditions had been swapped with made up illnesses. They were then asked to...

  10. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...origins of the system and explore advances that have happened since our original 2018 blog post. The origins... second generation of iGluSnFR, the Looger lab swapped eGFP for sfGFP to create SF-iGluSnFR. This improved...about other Fluorescent Biosensors A Practical Approach to Choosing the B(right)est Fluorescent Protein...

  11. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...perform the experiments to demonstrate that this approach was useful in humans. Then, one of them heard ...has been the realization that there is a great untapped potential to make science more efficient, open...activity is non-profit and pro bono. If this sounds appealing, join us and help make science a better place....

  12. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ... the self-assembling capability of sfCherry approximately 20 fold by directed evolution. They then optimized...concept that the designed used in FlipGFP can be applied to other fluorescent proteins to create an array...and high brightness. This reporter achieves approximately 100-fold increase in fluorescence after cleavage...

  13. Addgene Depositors Get More Citations

    Type
    Blog Post
    ... 3 Applied and Environmental Microbiology  1.6%  1.2% 0.75   2.3 4 2010 167  Applied and Environmental...Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology,...

  14. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...virtually) will make the relationship stronger.  If appropriate, invite them to speak for your organization, ...doesn’t work out, it’s flattering to ask.  Show appreciation. Send a thank you note or gift to someone who...with peers and people around you naturally. This approach is much more likely to pay off in the long run...

  15. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...actually making it happen would increase dramatically. I’m not sure what the best approach to doing that will... communication about experimental methods and approaches. It’s very easy to think that the methods section...

  16. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...(Schrallhammer et al 2012). This made them an appealing target for bioengineering, but it wasn’t clear...actuators that could be used in a variety of applications. For example, you could use them to lyse cells...matrices) in response to pH changes. Beyond these applications, purified R bodies could also perform as actuators...

  17. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...flank the insert. You will need to know both the approximate size of the vector backbone as well as the predicted...you have loaded in each well will allow you to approximate the DNA mass of comparably intense samples of...the gel when the bromophenol blue dye line is approximately 75-80% of the way down the gel will ensure you...

  18. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...dream lab While none of us will feel completely happy at work all of the time, the goal is to spend as...the difference. Choose a lab that brings on the happy dance at least once in awhile. Acknowledgments...

  19. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...guidance and structure. I quickly learned that my approach needed to adapt based on individual needs. For... and disengagement. By tailoring my leadership approach to each person, I was able to create a more productive...tension between two team members with differing approaches—one detail-focused and the other big-picture ...

  20. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...usable product with two 5′ overhangs, so these approaches require higher concentrations of insert to succeed...intermediate won’t just have nicks: it may have gaps or overlapping regions of single-stranded DNA. But as long as...of multicomponent assembly, as fragments with overlapping sequence homology can be assembled in a unique...
Showing: 481 - 500 of 960 results