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We narrowed to 647 results for: CAR

Showing: 481 - 500 of 647 results
  1. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...in a 50 mL conical tube and dispose after use. Carefully pour the filtered and diluted cell culture supernatant...Resin will appear compacted after centrifugation. Discard the flow through into a waste container. Add 5 ... 2 min to remove PBS. Repeat Steps 30–31 3x , discarding buffer from the collection tube each time. Place...Centrifuge at 1000 x g for 2 min to collect the sample. Discard column after use. Determine antibody concentration...min at RT . Centrifuge at 3100 x g for 8 min . Discard the flow through into a waste container. Add the...times to mix. Centrifuge at 3100 x g for 8 min . Discard the flow through into a waste container. Repeat...additional times to ensure a full buffer exchange. Discard the flow through into a waste container. Fill the...
  2. How Addgene Creates Content

    Type
    Blog Post
    ...Additional Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins...
  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...other AAV. Therefore, one should aim to keep their cargo below 4.4kB (with the ITRs bringing total genome...
  4. Antibodies 101: Isotypes

    Type
    Blog Post
    ...domain.   Check out our new Isotype trading cards!     References and resources References Janeway...
  5. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...obvious component of a biosensor is the thing that you care about within your cellular or animal system. This...
Showing: 481 - 500 of 647 results