We narrowed to 573 results for: Cit

1. The time and cost required to make a plasmid

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   ...finally dive deep into generating the data you’re excited about. When we were closing in on the one million...

2. All in a Twist: dsRNA

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   ...advancement over previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources...

3. What's New in CRISPR - November 2021

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   ... Holmes Several labs have recently shared some exciting plasmid tools to optimize prime editing systems...

4. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing...

5. Visualizing Protein Turnover In Situ

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   ...specific endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger, Eugenia Rojas...

6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...through the loss of the F plasmid, succumb to the toxicity of CcdB.   Figure 1: Image from Bernard...

7. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...

8. Giving gRNAs a Facelift - Synthetic and Beyond

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   ...CRISPR RNAs to improve Gene-Editing Activity and Specificity. JACS 144, 12584-12594 (2022). https://doi/pdf...

9. Summer SciComm: Preprints

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   ... scientific communication needs. The most often-cited reasons researchers might not post a preprint are...

10. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider...

11. Plasmids 101: Golden Gate Cloning

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    ...parts in different combinations, or other new and exciting applications, this system is an incredibly powerful...

12. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...articles. Then just start reading and following citations through the literature until you have all of the...

13. Working with Nuclear Receptors

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    ...the body through the bloodstream, and eventually elicit their actions on other tissues. At the Nuclear ...

14. Design Tips for Prime Editing

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    ...’s all our tips for today! Each tip includes a citation to the paper it was drawn from. If we’ve piqued...

15. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    .... It can be used to target lncRNAs with high specificity, particularly in applications requiring smaller...

16. Plasmids 101: The Wide World of Natural Plasmids

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    ...to explore Mother Nature’s toolbox for new and exciting molecular technologies!  This post was originally...

17. Making CRISPR Plasmids Using Fragmid

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    ...which you can also deposit with Addgene!) We’re excited to help the Doench lab share this new tool and ...

18. Progress Towards a PAM-Free CRISPR

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    ...and base editing applications, with high editing capacity in both contexts for NRN PAMs and to a lesser ...

19. CRISPR in the Clinic

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    ...Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood...

20. High Complexity Golden Gate Assembly with NEB’s DAD

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    ...can find for those parameters. For even more specificity, you can exclude specific sequences or set a ...