We narrowed to 570 results for: Cit
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TypeBlog Post... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...
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Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...through the loss of the F plasmid, succumb to the toxicity of CcdB. Figure 1: Image from Bernard... -
Antibodies 101: Fab Fragments
TypeBlog Post...when used in structural studies and reduces immunogenicity when used in vivo. Of course, the lack ... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...results to rapamycin inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing... -
Visualizing Protein Turnover In Situ
TypeBlog Post...specific endogenous proteins in any cell type. Exciting! Many thanks to our guest blogger, Eugenia Rojas... -
What's New in CRISPR - November 2021
TypeBlog Post... Holmes Several labs have recently shared some exciting plasmid tools to optimize prime editing systems... -
Summer SciComm: Preprints
TypeBlog Post... scientific communication needs. The most often-cited reasons researchers might not post a preprint are... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...parts in different combinations, or other new and exciting applications, this system is an incredibly powerful... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...articles. Then just start reading and following citations through the literature until you have all of the... -
Working with Nuclear Receptors
TypeBlog Post...the body through the bloodstream, and eventually elicit their actions on other tissues. At the Nuclear ... -
CRISPR in the Clinic
TypeBlog Post...Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...can find for those parameters. For even more specificity, you can exclude specific sequences or set a ... -
Viral Vectors 101: Virus Safety
TypeBlog Post... or 1% iodine are useful. Some organizations or cities may require a higher percentage of the decontaminate... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...Additional Resources on the Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable gRNAs ... -
Design Tips for Prime Editing
TypeBlog Post...’s all our tips for today! Each tip includes a citation to the paper it was drawn from. If we’ve piqued... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post.... It can be used to target lncRNAs with high specificity, particularly in applications requiring smaller... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...to explore Mother Nature’s toolbox for new and exciting molecular technologies! This post was originally... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...which you can also deposit with Addgene!) We’re excited to help the Doench lab share this new tool and ... -
Engaging with science and society at pgEd
TypeBlog Post...to non-technical audiences. Many universities or cities have organizations (often volunteer-based) that...