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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 772 results for: Dos

Showing: 481 - 500 of 772 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...small probes that are entirely genetically encoded do not exist, but the development of non-natural fluorescent...functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal fusions (Adjobo-Hermans...

  2. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...RGD-binding integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the...function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived antibodies fall into...

  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ... started!  I learned a lot about creative ways to do science communication and outreach - if you'd like...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies.... The simplest version would be to use cells that do not express your protein of interest endogenously...

  9. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...less than an hour. However large proteins typically do not transfer as well using this method.  Finally,...overexpression systems) while negative controls are those that do not naturally express the protein or have had gene...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...

  17. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid...multiple target genes in Escherichia coli. Koffas Do you have suggestions for other plasmids that should...pyogenes EGFP Zoldos pdCas9-DNMT3A-PuroR 71667 Mammalian U6 yes, methylation S. pyogenes Puro Zoldos pX330-U6... Mammalian U6 yes, methylation S. pyogenes Puro Zoldos pBLO1811_Cas9_noNLS_human 74490 Mammalian U6 yes...

  19. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide RNAs...
Showing: 481 - 500 of 772 results