...USA and Europe, something that I would not able to do without a program like this. However, I realized ... she does research at UK in the same field that I do: prostate cancer. She has guided me in my next steps...

...remember to step back and ask, "Why did I start doing this in the first place?" Many thanks to our guest...

... started!  I learned a lot about creative ways to do science communication and outreach - if you'd like...

...methods to quantify DNA? Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by...

...about changes influenced by feedback. If you don't do this, employees will stop providing feedback pretty...

...limit, some like prime editing's PE2 enzyme do not. So how do you fit a big gene into a tiny vector like...

...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies.... The simplest version would be to use cells that do not express your protein of interest endogenously...

...less than an hour. However large proteins typically do not transfer as well using this method.  Finally,...overexpression systems) while negative controls are those that do not naturally express the protein or have had gene...

...repair, these methods do not involve double strand break (DSB) formation and do not require donor DNA,...

...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...

... infect and then kill bacteria they enter. But to do so, the phage must recognize a specific part on the...

...years of incredible success, and for believing, as we do, that sharing speeds science.  This year, Chonnettia...

...later. Protein purification specialists recommend doing some research, notably determining the subcellular...

...business partners, without whom Addgene could not do what it does.  Melina talked about her grandfather...

...much international attention. Their small numbers do not, however, make these patients suffer any less...

...recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety...

...you high-quality educational resources to help you do better research. CRISPR is an incredibly fast-moving...

...with the request in your Addgene account.  Orders do not need to be placed immediately upon request approval...

...species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide RNAs...

...sharing impact grow beyond COVID-19. But, we can’t do this alone. If you have deposited plasmids at Addgene...