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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 770 results for: Dos;

Showing: 481 - 500 of 770 results

  3. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ... started!  I learned a lot about creative ways to do science communication and outreach - if you'd like...

  5. Plan Your Experiment

    Type
    Guide
    ... model a specific disease or process of interest. Do you want to: Generate complete and permanent loss...target cells are not transcribing DNA to mRNA, nor do they need to translate mRNA to protein. Instead, ...a lack of efficient target cleavage in cells that do express both Cas9 and gRNA. Before choosing a validation...using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA method described...

  7. Antibodies 101: Validation

    Type
    Blog Post
    ...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies.... The simplest version would be to use cells that do not express your protein of interest endogenously...

  9. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...less than an hour. However large proteins typically do not transfer as well using this method.  Finally,...overexpression systems) while negative controls are those that do not naturally express the protein or have had gene...

  11. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...

  16. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid...multiple target genes in Escherichia coli. Koffas Do you have suggestions for other plasmids that should...pyogenes EGFP Zoldos pdCas9-DNMT3A-PuroR 71667 Mammalian U6 yes, methylation S. pyogenes Puro Zoldos pX330-U6... Mammalian U6 yes, methylation S. pyogenes Puro Zoldos pBLO1811_Cas9_noNLS_human 74490 Mammalian U6 yes...
Showing: 481 - 500 of 770 results