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  1. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...transfection drawbacks: transient expression and needing to work with a transfectable cell line. 2) They... process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression...

  2. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...transcription >1,000-fold when activated. Lag time The time needed to induce a given promoter varies. Promoters that...inducer will have higher lag time due to the time needed for transcription/translation. Many thanks to ...

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...that there was a prior version of this plasmid. Indeed the first version is the 15th most popular plasmid...packaging, reverse transcription, and integration needed for the production of lentiviral particles. It’...

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...you would be oversized even before considering the need for some manner of polyA. In this scenario, you ... labeling at various doses.  As always, you will need to discuss injection volumes with your IACUC committee...

  5. Hot Plasmids - February 2022

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...most requested plasmids into lentiviral preps to speed up COVID-19 research even further. The following...different patterns of expression, researchers often need to determine these transduction patterns empirically...

  7. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...like the right approach for your experiment, you’ll need to decide exactly which type of virus to use. Types... viral particles inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses...

  8. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... price. With this increased complexity comes the need (and/or opportunities) to modify the device for ...components are adapted and combined with creativity and feedback from the community to create state-of-the-art ...

  9. Clear and Accessible Writing

    Type
    Blog Post
    ...writing applied to alt text. We also were aware of the need for clear, accessible writing in scientific writing...are blurred after centrifugation and the syringe needle is inserted bevel-up at the interface between the...

  10. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...biologists. Almost as soon as I began, I realized that I needed a system to keep track of the files that I was ...take to help you plan your analysis. There is no need to go into a lot of detail – the details will be...

  11. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...useful in generating the mutant cell lines that one needs to do disease modeling in stem cells. Addgene: Tell...TALEN kit includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available...

  15. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... and by offering advice on areas where you might need some help. Today in our transferable skills guide...revealed another issue in the process, you might need to go back to the beginning, reassess the situation...

  16. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of...antibody composition available. If you’re going to need a large quantity of antibody for your experiment...

  18. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...complementary site in the genome where it will proceed to generate a blunt DNA double strand break. Cas9...stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction...

  19. Addgene: The First Twenty Years

    Type
    Blog Post
    ...agreements and electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive..., with Addgene’s support, could fill a community need for high-quality service, open data, and increased...
Showing: 481 - 500 of 816 results