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We narrowed to 825 results for: EED

Showing: 481 - 500 of 825 results

  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...biologists. Almost as soon as I began, I realized that I needed a system to keep track of the files that I was ...take to help you plan your analysis. There is no need to go into a lot of detail – the details will be...

  2. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...useful in generating the mutant cell lines that one needs to do disease modeling in stem cells. Addgene: Tell...TALEN kit includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    ...agreements and electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive..., with Addgene’s support, could fill a community need for high-quality service, open data, and increased...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...complementary site in the genome where it will proceed to generate a blunt DNA double strand break. Cas9...stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction...

  9. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... and by offering advice on areas where you might need some help. Today in our transferable skills guide...revealed another issue in the process, you might need to go back to the beginning, reassess the situation...

  10. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of...antibody composition available. If you’re going to need a large quantity of antibody for your experiment...

  13. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... introduce Cre into cells of interest. What if I need two separate recombination events? One potential...

  14. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...promoters, N’ and/or C’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead...backbone and genes contain only the Bbsl cut sites needed for the Fragmid cuts, clone in the appropriate ...

  15. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...dollars on a new secondary antibody if you don’t need it.  If, on the other hand, your target is expressed...approach, that is simple - but what about when you need to use an indirect (primary and secondary antibody...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...of H2B-FT consistently predicted the cell cycle speed across these different cell types. In one example...

  17. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Epigenetic... regulators or chromatin-modifying enzymes. The speed and flexibility of the single-pot system will allow...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...The C. elegans community has always emphasized the need for open science and collaboration. The field already...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...
Showing: 481 - 500 of 825 results