We narrowed to 821 results for: EED

...that there was a prior version of this plasmid. Indeed the first version is the 15th most popular plasmid...packaging, reverse transcription, and integration needed for the production of lentiviral particles. It’...

...similar to the widely used FRET, but it eliminates the need for an excitation light source. Instead, a luciferase...Saito, Kenta, et al. “Luminescent proteins for high-speed single-cell and whole-body imaging.” Nat Commun....

...transfection drawbacks: transient expression and needing to work with a transfectable cell line. 2) They... process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression...

...transcription >1,000-fold when activated. Lag time The time needed to induce a given promoter varies. Promoters that...inducer will have higher lag time due to the time needed for transcription/translation. Many thanks to ...

...you would be oversized even before considering the need for some manner of polyA. In this scenario, you ... labeling at various doses.  As always, you will need to discuss injection volumes with your IACUC committee...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein...

...biologists. Almost as soon as I began, I realized that I needed a system to keep track of the files that I was ...take to help you plan your analysis. There is no need to go into a lot of detail – the details will be...

...useful in generating the mutant cell lines that one needs to do disease modeling in stem cells. Addgene: Tell...TALEN kit includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available...

...creatine. In order to reach the brain, creatine needs to be transported from the blood and cross the blood...effector functions. It’s an important process that we need to understand so that we could possibly develop ...

...used to the idea of general interfaces and the freedom to test out lots of combinations, to end up finding...these principles in a biological setting, we first need to define what features would allow for the best...

...agreements and electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive..., with Addgene’s support, could fill a community need for high-quality service, open data, and increased...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid... to traditional Gateway™ cloning, the GOI first needs to be cloned into an entry vector. The 2in1 cloning...

...is touring. But you know what a touring musician needs? A backstage team. Meet the microenvironment, a ...cancer microenvironment Ever since Stephen Paget’s “seed and soil” theory in 1889, microenvironments have...

... easier for you to find and use the plasmids you need. Below you'll find our hot plasmid articles from...TRIM21 is sufficient for TRIM-Away, and you only need to deliver the target antibody. TRIM-Away reagents...

...complementary site in the genome where it will proceed to generate a blunt DNA double strand break. Cas9...stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction...

... and by offering advice on areas where you might need some help. Today in our transferable skills guide...revealed another issue in the process, you might need to go back to the beginning, reassess the situation...

...variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of...antibody composition available. If you’re going to need a large quantity of antibody for your experiment...

...experimental manipulations, saving the time and money needed to develop new lines. Using nanobodies to create...experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted...

...Voigt’s group has also been assembling the toolbox needed for anyone to begin considering genetic engineering...identify and target a malignant cell, then you’d need the ability to turn on genes at different times....

...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... introduce Cre into cells of interest. What if I need two separate recombination events? One potential...