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We narrowed to 821 results for: EED

Showing: 481 - 500 of 821 results
  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...that there was a prior version of this plasmid. Indeed the first version is the 15th most popular plasmid...packaging, reverse transcription, and integration needed for the production of lentiviral particles. It’...
  2. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...similar to the widely used FRET, but it eliminates the need for an excitation light source. Instead, a luciferase...Saito, Kenta, et al. “Luminescent proteins for high-speed single-cell and whole-body imaging.” Nat Commun....
  3. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...transfection drawbacks: transient expression and needing to work with a transfectable cell line. 2) They... process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression...
  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...transcription >1,000-fold when activated. Lag time The time needed to induce a given promoter varies. Promoters that...inducer will have higher lag time due to the time needed for transcription/translation. Many thanks to ...
  5. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...you would be oversized even before considering the need for some manner of polyA. In this scenario, you ... labeling at various doses.  As always, you will need to discuss injection volumes with your IACUC committee...
  6. Hot Plasmids - February 2022

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein...
  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...biologists. Almost as soon as I began, I realized that I needed a system to keep track of the files that I was ...take to help you plan your analysis. There is no need to go into a lot of detail – the details will be...
  8. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...useful in generating the mutant cell lines that one needs to do disease modeling in stem cells. Addgene: Tell...TALEN kit includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available...
  9. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...used to the idea of general interfaces and the freedom to test out lots of combinations, to end up finding...these principles in a biological setting, we first need to define what features would allow for the best...
  10. Addgene: The First Twenty Years

    Type
    Blog Post
    ...agreements and electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive..., with Addgene’s support, could fill a community need for high-quality service, open data, and increased...
  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...complementary site in the genome where it will proceed to generate a blunt DNA double strand break. Cas9...stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction...
  12. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... and by offering advice on areas where you might need some help. Today in our transferable skills guide...revealed another issue in the process, you might need to go back to the beginning, reassess the situation...
  13. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of...antibody composition available. If you’re going to need a large quantity of antibody for your experiment...
  14. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... introduce Cre into cells of interest. What if I need two separate recombination events? One potential...
Showing: 481 - 500 of 821 results