We narrowed to 806 results for: Eng;

1. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   Blog Post

   ...Bacteria: CRISPR Methods for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing...and CRISPR History and Development for Genome Engineering  ...

2. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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   ...widely available imaging platform: ultrasound. Challenges of in vivo imaging with existing bacterial reporters...genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form ...

3. Bringing Sustainable Practices to the Lab: Recycling

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   ...for laboratory waste symposium, sponsored by New England Biolabs and Labconscious. I was eager to learn ...Labconscious. If you’re inspired by this post then I challenge you to take to action! These days, it’s important...

4. Embracing Serendipity: A Crucial Element in the PhD Journey

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   ...your primary focus lies within a biological lab. Engaging in such seminars exposes you to discussions beyond...release and mental freedom. Practical approaches Engaging in mindful meditation, taking field trips, and...

5. Management for Scientists: Managing vs. Leading

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   Blog Post

   ...motivating their doers by ensuring they are as engaged as possible. To learn more about being an effective... and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders...

6. Management for Scientists: What Makes a Good Manager Anyway?

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   Blog Post

   ... the successes of the team. A strong sense of engagement in the work Behind-the-scenes support from their...identify the work style of each team member) Need challenging work, opportunities to pursue and problems to...

7. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   ...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples...,241 amino acids long, has optimal sgRNA spacer length of 22-25 nts, and is thermostable between 31-100...

8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   ...Because mNeonGreen wavelengths lie between the green and yellow fluorescent protein wavelengths, it can be imaged...

9. RUBY-Red Siliques

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   ... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Additional resources on the Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis...

10. Hot Plasmids and Viral Preps - September 2021

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    Blog Post

    ...metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments for mammalian...mammalian cell lines. The Turner Lab Human messenger-RBP sgRNA Library targets human mRNA binding proteins with...

11. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post

    ...controls available when needed. SnapGene has been engineered to be easy and enjoyable to use. Development ...annotation process. Many of the best ideas come from engaging with users of the software. We welcome suggestions...

12. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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    ...screening platform, Oliver Griesbeck’s lab set out to engineer a new FP. Their aim - to red-shift the boundaries...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across...

13. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    ...are single domain antibody fragments typically engineered from heavy-chain only antibodies found in camelids...catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX) that labels proximal proteins...

14. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    ...23817127. PubMed Central PMCID: PMC3713494.  Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal...reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke, Meng-Tsen, and Takeshi Imai. "Optical clearing and index...

15. Fluorescent Biosensors for Measuring Autophagic Flux

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    ...Regardless of excitation wavelength, Keima emits at 620 nm. The ratio of signal strength excited at 586 nm to...

16. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    ...staining can be a challenge in the best of cases. But it can be particularly challenging when your only ...

17. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    Blog Post

    ...using sgRNAiBAR. Image from Zhu et al., 2019.  Wensheng Wei's lab at Peking University has developed a...recombination sites in the construct to different lengths, the density of cells that are labeled can be altered...

18. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post

    ...other media outlets (AAAS Mass Media Science & Engineering Fellows Program). Science journalism programs...the Advancement of Science. She is enjoys broad engagement through science communication with a variety ...

19. Addgene's Top 10 Blog Posts of 2015

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    Blog Post

    ...solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in...validation process for CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help...

20. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post

    ...fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence, cell... by directed evolution. They then optimized the length of both linkers to generate a reporter that would...