Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 527 results for: HA tag

Showing: 481 - 500 of 527 results

  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... exposure to increased intracellular calcium. It has been applied to mark active populations of neurons...using viral vectors! One of the most significant advantages of viral delivery is the potential for long-term...results in calcium assays. There’s another crucial advantage: tissue-specific targeting. By employing various...

  2. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... Prize in Chemistry for his work. Since then PCR has become a standard and essential practice in molecular...generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction, qPCR...interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics qPCR Quantify starting... PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase...

  3. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... proteins, respectively. A plasmid's copy number has to do with the balance between positive and negative...for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12...

  5. Plasmid Cloning by PCR

    Type
    Blog Post
    ...plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

  6. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...individual who is a local expert in that task (or has the bandwidth to become an expert) the end result...get more done. There are also a host of other advantages to practicing effective delegation skills: ...

  7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...regions of the gene is also useful in case the gene has a weird mutation that you didn’t know about (which...and is a testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months... the bands on your gel and get an idea of the percentage of cells in the population that have an edit....

  9. How to Lead a Great Meeting

    Type
    Blog Post
    ...this meeting is to _________." Everyone in the room has likely just run in from some other commitment. An... what you need to accomplish to get to the next stage, and then adjourn. Define the tone of the meeting...

  10. Pooled Library Amplifications

    Type
    Blog Post
    ...replicate faster. For this reason, if your library has inserts of different sizes (such as a transcription... is less than that of electroporation, but an advantage is that chemical transformations are generally...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  11. Antibodies 101: Flow Compensation

    Type
    Blog Post
    .... This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells and cancer ..., unlike voltages, you can adjust compensation values later in your analysis, unlike voltages.  Figure...

  12. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change (from...of the cellular DNA… forever!  This can be an advantage for researchers looking to generate long-lasting...lentiviral vector: 1. No risk of insertional mutagenesis Lentiviral vectors tend to integrate into transcriptionally...2002), thus increasing the risk for insertional mutagenesis. This integration could lead to an undesirable...harbor these unwanted interruptions. Insertional mutagenesis is especially concerning in clinical settings...acute lymphoblastic leukemia due to insertional mutagenesis (Howe et al., 2008). 2. Transient expression ...cells, as they avoid the risk of insertional mutagenesis while still allowing for stable expression of...

  13. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...be used to determine how long a pool of FP timers has been expressed, either in whole cells or as a pool...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...

  15. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular...useful for viral reproduction, and for causing contagious infection; however this would not be great for...

  16. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...since bacteria won’t divide immediately after it has been heat-shocked or electrically zapped. As a result...cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on ...

  17. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in terms of... popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity...research is still in the pre-clinical/early clinical stages. Researchers are also working to make lentiviral...

  18. CRISPR Antimicrobials

    Type
    Blog Post
    ...caveat that phages are very diverse, and each phage has a narrow bacterial host range. To further potential...antibiotic resistance. Advantages of CRISPR over traditional antibiotics The potential advantages of CRISPR over...

  20. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...
Showing: 481 - 500 of 527 results