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We narrowed to 520 results for: HA tag

Showing: 481 - 500 of 520 results

  2. How to Lead a Great Meeting

    Type
    Blog Post
    ...this meeting is to _________." Everyone in the room has likely just run in from some other commitment. An... what you need to accomplish to get to the next stage, and then adjourn. Define the tone of the meeting...

  3. Pooled Library Amplifications

    Type
    Blog Post
    ...replicate faster. For this reason, if your library has inserts of different sizes (such as a transcription... is less than that of electroporation, but an advantage is that chemical transformations are generally...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  4. Antibodies 101: Flow Compensation

    Type
    Blog Post
    .... This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells and cancer ..., unlike voltages, you can adjust compensation values later in your analysis, unlike voltages.  Figure...

  5. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change (from...of the cellular DNA… forever!  This can be an advantage for researchers looking to generate long-lasting...lentiviral vector: 1. No risk of insertional mutagenesis Lentiviral vectors tend to integrate into transcriptionally...2002), thus increasing the risk for insertional mutagenesis. This integration could lead to an undesirable...harbor these unwanted interruptions. Insertional mutagenesis is especially concerning in clinical settings...acute lymphoblastic leukemia due to insertional mutagenesis (Howe et al., 2008). 2. Transient expression ...cells, as they avoid the risk of insertional mutagenesis while still allowing for stable expression of...

  6. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...be used to determine how long a pool of FP timers has been expressed, either in whole cells or as a pool...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...

  8. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...since bacteria won’t divide immediately after it has been heat-shocked or electrically zapped. As a result...cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I Resources on ...

  9. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Noninfectious ARK mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious...namely that they can cause cancer via insertional mutagenesis (which HIV does not do). Furthermore, molecular...useful for viral reproduction, and for causing contagious infection; however this would not be great for...

  10. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in terms of... popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity...research is still in the pre-clinical/early clinical stages. Researchers are also working to make lentiviral...

  11. CRISPR Antimicrobials

    Type
    Blog Post
    ...caveat that phages are very diverse, and each phage has a narrow bacterial host range. To further potential...antibiotic resistance. Advantages of CRISPR over traditional antibiotics The potential advantages of CRISPR over...

  13. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...

  15. RNA Extraction Without A Kit

    Type
    Blog Post
    ...several reasons why doing something without a kit has advantages: less plastic waste, less expense, and less...

  16. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... the RNA backbone to increase stability.  Nature has evolved ways to prevent degradation of important ...less off-target events. While both have similar advantages, bridged nucleic acids with N-methyl substitutions...

  18. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...assistance in the grant application process, she has faith in you! Additionally, keep in mind that universities...of current methodologies. This section sets the stage for why your funded proposal will address these ...

  19. Engaging with science and society at pgEd

    Type
    Blog Post
    ...Department of Genetics of Harvard Medical School, has a mission of raising awareness and starting conversations...who are interested in such careers should take advantage of their academic environment and attend seminars...

  20. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...availability of both wild-type and mutant loxP sites has allowed scientists to leverage this system in new...site-specific recombination. The FLEx switch takes advantage of the orientation specificity of the site-specific...
Showing: 481 - 500 of 520 results