...operations for all ongoing processes are entirely self-supporting. We hire more staff as we need them to meet... were closed and not receiving packages. So the entire team mobilized to figure out how to deliver when...

...rate discovery rate, conventional CRISPR screens require a low multiplicity of infection (MOI) during cell... tools for doing this were labor-intensive and required using manipulations such as injection of precise...drives gene expression. Because calcineurin is required for virulence in pathogenic fungi, it is an attractive...

... Using structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues from The...violet range. For example, mWasabi can be used with Sapphire (excitation maximum 399 nm) because it does not... at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a monomeric, bright and photostable...

...using AAV on our blog. Image: The final and required step requires submission of an experimental image result...expression of AAV cargo? Did you use GCaMP expression? Direct Fluorescence? Immunohistochemistry? Did you use...

...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics... See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5... sensors provide the spatiotemporal resolution required for precise in vivo measurements of dopamine (...

...does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The... proofreading polymerases will also remove all unpaired 3’ ends in your PCR product. If you need to decrease...Additional Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to...

...rare diseases,” said Claudia OuYang, BeHEARD Co-Director in a release. The research teams studying four...says that the lab got interested in XP out of a desire to understand the early events in the transformation...variants and strategies out there. This will help us acquire a lot of those plasmids so that we can try many...

...Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering...  To start, the average health science startup requires a great deal more capital to get off the ground... your name (P-H-D).  Finally, having spent your entire career in science, you are likely lacking the necessary...

...Addgene Blog Mentoring for Scientists Series. The entire series and additional resources can be downloaded...responsibility of arranging meetings and setting the direction in a mentoring relationship falls on the shoulders...Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the "Forced Choice...

...communication. A great approach to fully understand and acquire competitive skill sets in a myriad of professions...job sites to find open positions and view their required qualifications and skill sets Taking the time...graduate students and postdocs are currently getting hired? Let us know what topic you'd like to learn more...

... Just enter the DOI of a paper and select your desired formatting style. This tool is great for generating...with others. The Web Importer plug-in lets you directly import papers from web pages and academic databases...the Zotero Connector, papers can be saved from Firefox or Chrome browsers with just one click. Papers ...

...BosLab and previous laboratory experience is not required. Our members are skilled with audiovisual media...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

.... The tools are a lifesaver when your research requires cell culture and include a counter, lab timer,...Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app contains a ...the app is quite helpful and provides a list of directions including which buffers to use and that indicates...

...scope of the meeting: Are you meeting to design an entire experiment and delegate tasks to lab mates or to... to discuss a potential new research direction you’d like to pursue? Undefined scope can lead to long ... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ...

...tropism. Briefly, different envelope plasmids can direct the production of virus with various tropisms.  ...therefore, reduces the number of plasmids that are required in the transfection step of the viral packaging...incompetent? Yes, as with lentiviruses, the genes required for γ-retroviral packaging and transduction are...

...biological phenomena by generating an output which is directly proportionate to the amount of a given factor ...either factors as proxies for a cellular process or directly measure a cellular process. Cellular phenomena...type of cells the viral particles infect. This can direct your biosensor to specific tissues or cell (sub...

...transfect’, such as primary cells. They can also be directly injected into hard-to-reach organs, such as the...like replication incompetent viral particles, are required. We will be covering these safety measures in ...dividing cells, as their access to the host genome requires breakdown of the nuclear envelope, which occurs...

...truly believes that sharing speeds science, and our entire mission is to enable that process. Our repository... made most of the Antibody Guide figures. This requires a fair amount of communication between our content...of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, for example,...

...it like winter tires in different conditions: the more flexible rubber of winter tires give a better grip...recombinant proteins for industrial or research purposes requires stable host cells (the simplest example would ...

...display-based directed evolution to create APEX2. They have now applied the same directed evolution technique... tagging proximal proteins within minutes, but requires H2O2, which can be toxic in living cells. BioID...