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We narrowed to 972 results for: LAT;

Showing: 481 - 500 of 972 results

  1. Hot Plasmids - November 2023

    Type
    Blog Post
    ...PE6b was very efficient for short, unstructured templates and significantly reduced undesired edits (indels...complex edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing...potential to reduce negative side effects. This platform offers a promising approach for experimental therapies...illuminated with blue light, catalyzing the biotinylation of nearby proteins. B) TransitID uses sequential... et al. (2023). Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control...

  2. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ... to grow on plates or in liquid culture to amplify the library, followed by DNA isolation. The amplified...screening-scale transduction of cells, resulting in cell population mutated by the viral pooled library. These cells...payload is adequately represented in the cell population, you’ll need to infect many more cells than the...Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale...

  3. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...Secretary of Labor Thomas Perez updated the overtime regulations of the FLSA. The exemption salary was set at ... postdocs are at each of these institutions (populations potentially from 1 to 5761, according to the ...research on these institutions, we can say with relative certainty that just over 40% of current postdocs...for academia Changes are afoot for the postdoc population, and it is unclear how this will all shake out...effects the new laws will have on the postdoc population. In the current funding climate combined with...

  4. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...in your favorite neuronal population and track connections to that population. Limitations of common genetically...to define those that target specific neuronal populations in the brain. The Allen Institute and the Janelia...also been important in mapping, monitoring, and manipulating neuronal circuits. Epitope tags are short antigenic...cells. They can be used for protein localization, isolation, and tracking experiments (Rizzo, 2009). With ...

  5. March for Science

    Type
    Blog Post
    ...can do and, in this way, science has always been related to government and, consequently, politics. More...Inclusion of data informing policy specifically related to the following subjects: Climate change Vaccines...Healthcare Science education To make scientists relatable (17, 20). This is an effort to show the general...all you need to do is select your state. State Legislatures: To find the website to contact representatives...Lawmakers need to use a scientific approach to formulating views Pew Research Center: Public and Scientists...

  6. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ... universities, private companies, and federal regulators. But the speed is impressive on a larger scale... The first base editor was reported three years later in 2016 (Komor et al., 2016). Advancing from this...create C→T base edits at a target locus. A year later, an A→G base editor (ABE) followed based on similar...et al., 2020; Richter et al., 2020).  Five years later, three of these variants (ABE8.8, ABE8.20, and ABE8e... assessed for off-target risks, such a modular platform could accelerate the development of genetic therapies...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Having successfully navigated the trials and tribulations of the process, I’ve provided guidance and mentoring...interviews but no job offers, is a failure to clearly articulate one's talents and ideas. Some key points from...question? This should be something that anyone could relate to, that people would agree is important to understand...provide the first defined model system that we can manipulate to study pathology and therapies." This is...interview, your previous success will not necessarily translate into getting a job. Your papers and pedigree get...

  8. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...strategy for increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs ...acceptor of the transgene to help prevent the accumulation of such truncated proteins. Examples of use...homologous recombination approach delivers large donor templates using two sequential homology directed repairs... the gRNA target site that mediated the donor template’s integration so that the site is reconstituted...which results in the integration of a larger donor template.  Drawbacks: Transgene expression varies with...

  10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ..., generating new therapeutics, and sometimes formulating new biological concepts. However, rates of reproducibility... However, even simple procedures like pouring a plate or running a gel have intricacies that aren’t always...have plans to expand to other protein technologies later. Organizations such as BenchSci and FPbase are working...find information about research tools. BenchSci collates information about antibodies while FPbase curates... soon, the Center for Open Science does host a platform for pre-registration wherein researchers can submit...

  11. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...N-linked sialic acid4 AAV receptor (AAVR)2 Platelet-derived growth factor receptor (PDGFR) α and β3...and so on, although we often use abbreviated nomenclature (AAV1, AAV2, etc.) to distinguish the capsids... cells (Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied and most commonly...2023; Wu, Asokan, et al., 2006). AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency...retinal cells (Issa et al., 2023). AAV11 AAV11 is a relatively recent addition to the toolbox, with the first...

  12. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene Expression in Nonhuman...primate. Likewise, in vitro efficacy doesn’t always translate to in vivo efficacy. You’ll therefore need to ... strong early gene expression, but can become methylated over time, resulting in decreased expression ...consider. First, the titer required is inversely related to transduction efficacy: the higher the efficiency...

  13. Finding Your Perfect Job After University

    Type
    Blog Post
    ...Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new ... felt I needed more practical lab experience to later gain a full time position in a lab. I worked on ...left me optimistic that I would find a genetics related role soon after finishing my MSc. The masters program...I had received all the training and experience relating to good manufacturing practice (GMP) and good ...and decided to compromise and applied anyway. I later found out the job was with Addgene. I had used plasmids...

  14. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...investigator. In basic science (as opposed to translational, biomedical, or applied sciences), people conduct...everything there is to know is a strange and exciting revelation. Science in any field can generally be applied...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...students, women, BIPOC, and other minoritized populations. Research universities often have scholarships...

  15. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...antigen-antibody interactions in a plate-based format, making it suitable for comparing relative affinities across mutants...structure of your antibody-antigen complex as a template. This structural framework then guides the specific...their large-scale recombinant antibody production platform. IPI is a leading expert on antibody discovery..., & Kruse, A. C. (2018). Yeast surface display platform for rapid discovery of conformationally selective...

  16. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...broth and on the agar plates. Mycoplasma colonies are easily visible on the agar plates. This method is capable...detection, DNA staining, fluorescent tagging, agar plating, etc. Be wise to choose any of the techniques listed... (Uphoff and Drexler, 2011). Culturing on agar plates/broth is considered the ‘gold standard’ for detecting...prevent contamination rather than eradicating it later. Don’t be scared when mycoplasma infects your cell...

  17. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference...inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed repair (HDR) can be ...using a single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex...Candida albicans A new CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans...

  18. Science Communication for Everyone

    Type
    Blog Post
    ...like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate” could...different topics of science, especially disease related topics. Neurodegenerative and brain disorders are...illicit tampering” to general audiences while “manipulate” could mean “worked with” or “changed” to scientists...

  19. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...said. “It opens a new avenue of modifying and manipulating endogenous eukaryotic RNA.” Endless possibilities...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1...(1):7. doi: 10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview More...

  20. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...Expanding your network of relationships early and often is the most effective tactic a scientist in training...result of existing professional (and personal) relationships. However, most job seekers will and should apply...after the academic bench. Utilizing existing relationships as part of the job application process can dramatically...you can use alumni databases, friends, and even relatives to network your way to a hiring manager. Learn...
Showing: 481 - 500 of 972 results