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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 808 results for: MAK

Showing: 481 - 500 of 808 results

  2. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative ...misinformation in the wake of a global pandemic can make for a sore reminder of how far we have yet to go...

  3. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...science communication research.   This summer, we’re making time to talk about my favorite thing - science ...but the foundation of all good communication is making sure everyone’s on the same page. So for our first...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...extra extension time after the last cycle of PCR to make sure that the "A" gets added to all PCR products... the standard ligation reaction is fairly quick, make sure you stay organized and prepare everything you...

  5. Plasmid Cloning by PCR

    Type
    Blog Post
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

  7. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...?” and "What type of science careers would would make a good fit for me?" Whatever would I do next and...for sales and marketing jobs Like most choices we make, my job choices were mostly by chance. Luckily, ...

  8. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV ...and disruptive mutations and truncations that can make vector propagation and sequencing a headache. How...

  9. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-...restriction enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’...

  10. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...published in the same journals as those that did make deposits to Addgene.  Insomuch as different journals... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...tools out there to visualize genomic loci, what makes CRISPR-Sirius unique? Unlike fluorescent in situ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

  13. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety...engineer yeast to do similar things. The focus is to make yeast engineering accessible to everyone but these...

  15. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...control for a specific marker in your experiment. To make an FMO sample, you take a subset of your experimental... is prepared very similarly to a FMO sample: you make a sample from a combined subset of your experimental...

  16. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...then inserted into the host's genome and is used to make more virus. The most studied (and used in the lab...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ... Thus, the adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic ...immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  18. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...different constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that ...

  20. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify... are a few drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...
Showing: 481 - 500 of 808 results